Potential effects of Rhodococcus qingshengii strain djl-6 on the bioremediation of carbendazim-contaminated soil and the assembly of its microbiome.

As a widely used fungicide, the environmental fate of carbendazim and its residues in agricultural products have caused great concern. However, its effects on soil microbial communities are largely unknown. Herein, we used high-throughput sequencing to reveal the effects of high and low dose of carbendazim and its degrading strain, Rhodococcus qingshengii strain djl-6, on the composition, diversity, and interrelationship of soil bacterial and fungal communities in short- and medium-term under laboratory conditions. The results showed that carbendazim exhibited an increased negative impact on bacterial communities and reduced the proportion of dominant fungal phylum Ascomycota during a 14-day incubation period. Only the impacts of low-dose carbendazim (2 mg·kg-1 dry soil) on fungal community were weakened. Network analysis showed that carbendazim increased the connectivity and modularity of microbial co-occurrence networks. Strain djl-6 exhibited good potential for bioremediation of carbendazim-contaminated soils. Moreover, it driven the assembly of potential carbendazim-degrading consortia from indigenous microbial communities; and members of the genera Arthrobacter, Bacillus, Brevundimonas, Lysinibacillus, Massilia, Mycobacterium, Paenibacillus, and Pseudarthrobacter might be participated in the degradation of carbendazim. Taken together, our study provides a relatively comprehensive understanding of the effects of carbendazim and its degrading strain djl-6 on soil microbial communities.

• Publication year

  2021

• Venue

  Journal of Hazardous Materials

• Publication date

  2021-02-24

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1016/j.jhazmat.2021.125496PMID 33667802
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Tax4Fun2: prediction of habitat-specific functional profiles and functional redundancy based on 16S rRNA gene sequences

  2020cited by this paper
• Earth microbial co-occurrence network reveals interconnection pattern across microbiomes

  2020cited by this paper
• Microbial catabolism of lindane in distinct layers of acidic paddy soils combinedly affected by different water managements and bioremediation strategies.

  2020cited by this paper
• Enhanced elimination of dimethachlon from soils using a novel strain Brevundimonas naejangsanensis J3.

  2019cited by this paper
• The UNITE database for molecular identification of fungi: handling dark taxa and parallel taxonomic classifications

  2018cited by this paper
• Cropping practices manipulate abundance patterns of root and soil microbiome members paving the way to smart farming

  2018cited by this paper
• Residue dissipation and risk assessment of tebuconazole, thiophanate-methyl and its metabolite in table grape by liquid chromatography-tandem mass spectrometry.

  2018cited by this paper
• Non-target effects on soil microbial parameters of the synthetic pesticide carbendazim with the biopesticides cantharidin and norcantharidin

  2017cited by this paper
• Distinct succession patterns of abundant and rare bacteria in temporal microcosms with pollutants.

  2017cited by this paper
• The effective migration of Massilia sp. WF1 by Phanerochaete chrysosporium and its phenanthrene biodegradation in soil.

  2017cited by this paper
• Rhamnolipid-aided biodegradation of carbendazim by Rhodococcus sp. D-1: Characteristics, products, and phytotoxicity.

  2017cited by this paper
• Identification of the key amino acid sites of the carbendazim hydrolase (MheI) from a novel carbendazim-degrading strain Mycobacterium sp. SD-4.

  2017cited by this paper
• Complete genome sequence of a psychotrophic Pseudarthrobacter sulfonivorans strain Ar51 (CGMCC 4.7316), a novel crude oil and multi benzene compounds degradation strain.

  2016cited by this paper
• Modulation of macrophage functionality induced in vitro by chlorpyrifos and carbendazim pesticides

  2016cited by this paper
• FUNGuild: An open annotation tool for parsing fungal community datasets by ecological guild

  2016cited by this paper
• Microbial succession in response to pollutants in batch-enrichment culture

  2016cited by this paper
• Changes in soil microbial community structure and function associated with degradation and resistance of carbendazim and chlortetracycline during repeated treatments.

  2016cited by this paper
• Individual and combined effects of tebuconazole and carbendazim on soil microbial activity

  2016cited by this paper
• Soil and Rhizosphere Associated Fungi in Gray Mangroves (Avicennia marina) from the Red Sea — A Metagenomic Approach

  2015cited by this paper
• Reproductive and possible hormonal effects of carbendazim.

  2014cited by this paper
• Isolation, identification and cyfluthrin-degrading potential of a novel Lysinibacillus sphaericus strain, FLQ-11-1.

  2014cited by this paper
• Pesticide residues in fruits and vegetables from the Aegean region, Turkey.

  2014cited by this paper
• Isolation and Characterization of Carbendazim-degrading Rhodococcus erythropolis djl-11

  2013cited by this paper
• Nutrient enrichment increased species richness of leaf litter fungal assemblages in a tropical forest

  2013cited by this paper
• Carbendazim dissipation in the biomixture of on-farm biopurification systems and its effect on microbial communities.

  2013cited by this paper
• The SILVA ribosomal RNA gene database project: improved data processing and web-based tools

  2012cited by this paper
• Complete genome sequence and metabolic potential of the quinaldine-degrading bacterium Arthrobacter sp. Rue61a

  2012cited by this paper
• Response of soil bacterial community to repeated applications of carbendazim.

  2012cited by this paper
• Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing

  2012cited by this paper
• Brazilian monitoring programs for pesticide residues in food – Results from 2001 to 2010

  2012cited by this paper
• Microbial interactions: from networks to models

  2012cited by this paper
• Multiresidue method for the determination of 13 pesticides in three environmental matrices: water, sediments and fish muscle.

  2011cited by this paper
• Microbial degradation of aromatic compounds — from one strategy to four

  2011cited by this paper
• Dissipation of carbendazim and chloramphenicol alone and in combination and their effects on soil fungal:bacterial ratios and soil enzyme activities.

  2011cited by this paper
• Dynamics of Carbendazim Residue in Panax notoginseng and Soil

  2010cited by this paper
• Determination of pesticides in surface and ground waters by liquid chromatography-electrospray-tandem mass spectrometry.

  2010cited by this paper
• A scaling normalization method for differential expression analysis of RNA-seq data

  2010cited by this paper
• Biodegradation of carbendazim by a novel actinobacterium Rhodococcus jialingiae djl-6-2.

  2010cited by this paper
• Cloning and Biochemical Characterization of a Novel Carbendazim (Methyl-1H-Benzimidazol-2-ylcarbamate)-Hydrolyzing Esterase from the Newly Isolated Nocardioides sp. Strain SG-4G and Its Potential for Use in Enzymatic Bioremediation

  2010cited by this paper
• Influence of environmental factors on degradation of carbendazim by bacillus pumilus strain NY97-1.

  2009cited by this paper
• Microbial community structure and its functional implications

  2009cited by this paper
• Effect of carbendazim and pencycuron on soil bacterial community.

  2009cited by this paper
• [Characterization of a carbendazim-degrading Trichoderma sp. T2-2 and its application in bioremediation].

  2009cited by this paper
• Carbendazim induces a temporary change in soil bacterial community structure.

  2009cited by this paper
• The unseen majority: soil microbes as drivers of plant diversity and productivity in terrestrial ecosystems.

  2008cited by this paper
• Isolation and Characterization of a Carbendazim-Degrading Rhodococcus sp. djl-6

  2006cited by this paper
• The Use of Integrated Soil Microcosms to Assess the Impact of Carbendazim on Soil Ecosystems

  2004cited by this paper
• Down regulation of humoral immunity in chickens due to carbendazim.

  2003cited by this paper
• PAH-degradation by Paenibacillus spp. and description of Paenibacillus naphthalenovorans sp. nov., a naphthalene-degrading bacterium from the rhizosphere of salt marsh plants.

  2002cited by this paper
• Embryolethal and teratogenic effects of carbendazim in rats.

  2001cited by this paper
• Isolamento de fungos degradadores de carbendazim

  1999cited by this paper
• Acute exposure of female hamsters to carbendazim (MBC) during meiosis results in aneuploid oocytes with subsequent arrest of embryonic cleavage and implantation.

  1996cited by this paper
• Carbendazim-induced alterations of reproductive development and function in the rat and hamster.

  1990cited by this paper
• Diversity in tropical rain forests and coral reefs.

  1978cited by this paper
• The effect of benomyl and carbendazim on mitosis in hyphae of Botrytis cinerea Pers. ex Fr. and roots of Allium cepa L

  1975cited by this paper
• STUDIES ON LYSOGENESIS I

  1951cited by this paper
• Distributed under Creative Commons Cc-by 4.0 Vsearch: a Versatile Open Source Tool for Metagenomics

  year unknowncited by this paper

• Indigenous Rhodococcus-derived resuscitation-promoting factor reactivates VBNC cells and enhances PCB biodegradation in contaminated soil

  2026cites this paper
• THE EFFECT OF THE USE OF FUNGICIDES ON THE PRODUCTIVITY OF WINTER WHEAT AND THE MICROBIOLOGICAL ACTIVITY OF SOILS

  2025cites this paper
• Green, High-Throughput Determination of Multiple Classes of Pesticides in Paddy Water Employing Thin-Film Solid-Phase Microextraction (TF-SPME) and High-Performance Liquid Chromatography (HPLC)

  2025cites this paper
• Earthworm gut microbiota: A novel biocatalyst for the sustainable degradation of carbendazim in Agro-ecosystems

  2025cites this paper
• Advances in actinobacteria-based bioremediation: mechanistic insights, genetic regulation, and emerging technologies

  2025cites this paper
• Revealing the mechanism of Burkholderia sp. Pyr-1 driven removal of pyraclostrobin-contamination through soil microbial community function and assembly

  2025cites this paper
• Organic Fertilization Mitigates Long-Acting Herbicide Mesosulfuron-Methyl Effects via Rhizosphere Soil Bacterial Community Regulation in Wheat.

  2025cites this paper
• A promising alternative for sustainable remediation of carbendazim in aquatic environments

  2024cites this paper
• Carbendazim mycoremediation: a combined approach to restoring soil

  2024influential citation
• Mechanisms of benzene and benzo[a]pyrene biodegradation in the individually and mixed contaminated soils.

  2024cites this paper
• Response of earthworm enzyme activity and gut microbial functional diversity to carbendazim in the manured soil

  2024cites this paper
• Carbendazim adsorption on polyethylene microplastics and the toxicity mechanisms on cotton plants, soil enzyme activity and rhizosphere bacterial community under combined stress conditions

  2024cites this paper
• Microbes as carbendazim degraders: opportunity and challenge

  2024cites this paper
• Probiotics reshape the coral microbiome in situ without detectable off-target effects in the surrounding environment

  2024cites this paper
• Reducing the accumulation of cadmium and phenanthrene in rice by optimizing planting spacing: Role of low-abundance but core rhizobacterial communities.

  2024cites this paper
• Biosurfactant production by Rhodococcus ALDO1 isolated from olive mill wastes

  2024cites this paper
• Acid-modified corn straw biochar immobilized Pseudomonas hibiscus CN-1 facilitated the bioremediation of carbendazim-contaminated soil.

  2024cites this paper
• Genomic and phylotypic properties of three novel marine Bacteroidota from bare tidal flats reveal insights into their potential of polysaccharide metabolism

  2023cites this paper
• Biotransformation of carbendazim in cowpea pickling process.

  2023cites this paper
• The win-win effects of nitrification inhibitors on soil-crop systems: Decreasing carbendazim residues but promoting soil bacterial community diversities and stabilities and crop yields.

  2023cites this paper
• Immobilization of Klebsiella jilinsis strain 2N3 by corn straw biochar enhanced the degradation of nicosulfuron and restores the soil microbiome function and composition

  2023cites this paper
• Stenotrophomonas pavanii DJL-M3 inoculated biochar stabilizes the rhizosphere soil homeostasis of carbendazim-stressed rice.

  2023cites this paper
• Amino acid fertilizer (AAF) strengthens its fertilizer effects on crop yield and quality by recruiting beneficial rhizosphere microbes, which contributes to improved soil health.

  2023cites this paper
• Efficient biodegradation of multiple pyrethroid pesticides by Rhodococcus pyridinivorans strain Y6 and its degradation mechanism

  2023cites this paper
• Insight into functional mechanisms of percarbamide and nitrification inhibitors in degrading fungicide residues and shaping microbial communities in soil-plant systems.

  2023cites this paper
• Evaluation of Antibacterial and Antiviral Compounds from Commiphora myrrha (T.Nees) Engl. Resin and Their Promising Application with Biochar

  2023cites this paper
• Characterization and low-temperature biodegradation mechanism of 17β-estradiol-degrading bacterial strain Rhodococcus sp. RCBS9.

  2023cites this paper
• Different performance of pyrene biodegradation on metal-modified montmorillonite: Role of surface metal ions from a bioelectrochemical perspective.

  2022cites this paper
• Combined effects of spent mushroom substrate and dicyandiamide on carbendazim dissipation in soils: Double-edged sword effects and potential risk controls.

  2022cites this paper
• In situ bioremediation of petroleum hydrocarbon–contaminated soil: isolation and application of a Rhodococcus strain

  2022cites this paper
• High tolerance and degradation of fungicides by fungal strains isolated from contaminated soils

  2022cites this paper
• Rapid Detection of Carbendazim Residue in Apple Using Surface-Enhanced Raman Scattering and Coupled Chemometric Algorithm

  2022cites this paper
• Diversity of rice rhizosphere microorganisms under different fertilization modes of slow-release fertilizer

  2022cites this paper
• A Competitive Assay Based on Dual-Mode Au@Pt-DNA Biosensors for On-Site Sensitive Determination of Carbendazim Fungicide in Agricultural Products

  2022cites this paper
• Revealing the driving synergistic degradation mechanism of Rhodococcus sp. B2 on the bioremediation of pretilachlor-contaminated soil.

  2022cites this paper
• Rhodococcus: A promising genus of actinomycetes for the bioremediation of organic and inorganic contaminants.

  2022cites this paper
• A highly-sensitive sensor based on carbon nanohorns@reduced graphene oxide coated by gold platinum core-shell nanoparticles for electrochemical detection of carbendazim in fruit and vegetable juice.

  2022cites this paper
• Bioremediation of hazardous pollutants from agricultural soils: A sustainable approach for waste management towards urban sustainability.

  2022cites this paper
• Use of soil actinomycetes for pharmaceutical, food, agricultural, and environmental purposes

  2022cites this paper
• Degradation effect of carbendazim in soil by application with the microbial agent, Rhodococcus sp. 3-2

  2021cites this paper
• Enhanced bioremediation of sediment contaminated with polycyclic aromatic hydrocarbons by combined stimulation with sodium acetate/phthalic acid.

  2021cites this paper
• Actinobacteria: An eco-friendly and promising technology for the bioaugmentation of contaminants

  2021cites this paper
• Probiotics reshape the coral microbiome in situ without affecting the surrounding environment

  year unknowncites this paper