Phenotypic characterization of Adig null mice suggests roles for adipogenin in the regulation of fat mass accrual and leptin secretion

Summary Adipogenin (Adig) is an adipocyte-enriched transmembrane protein. Its expression is induced during adipogenesis in rodent cells, and a recent genome-wide association study associated body mass index (BMI)-adjusted leptin levels with the ADIG locus. In order to begin to understand the biological function of Adig, we studied adipogenesis in Adig-deficient cultured adipocytes and phenotyped Adig null (Adig−/−) mice. Data from Adig-deficient cells suggest that Adig is required for adipogenesis. In vivo, Adig−/− mice are leaner than wild-type mice when fed a high-fat diet and when crossed with Ob/Ob hyperphagic mice. In addition to the impact on fat mass accrual, Adig deficiency also reduces fat-mass-adjusted plasma leptin levels and impairs leptin secretion from adipose explants, suggesting an additional impact on the regulation of leptin secretion.

• Publication year

  2021

• Venue

  Cell Reports

• Publication date

  2021-03-09

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/j.celrep.2021.108810PMID 33691105PMCID 7966854
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Dysregulation of a long noncoding RNA reduces leptin leading to a leptin-responsive form of obesity

  2019cited by this paper
• Peroxisome Proliferator-Activated Receptor γ2 Controls the Rate of Adipose Tissue Lipid Storage and Determines Metabolic Flexibility

  2018cited by this paper
• Leptin and the maintenance of elevated body weight

  2018cited by this paper
• A Completely Reimplemented MPI Bioinformatics Toolkit with a New HHpred Server at its Core.

  2017cited by this paper
• Leptin's Physiologic Role: Does the Emperor of Energy Balance Have No Clothes?

  2017cited by this paper
• Phospholamban and sarcolipin: Are they functionally redundant or distinct regulators of the Sarco(Endo)Plasmic Reticulum Calcium ATPase?

  2016cited by this paper
• Genome-wide meta-analysis uncovers novel loci influencing circulating leptin levels

  2016cited by this paper
• The long road to leptin.

  2016cited by this paper
• Data on regulation of the gene for the adipocyte-enriched micropeptide Adig/Smaf1 by qPCR analysis and luciferase reporter assay

  2016cited by this paper
• Expression, regulation and functional assessment of the 80 amino acid Small Adipocyte Factor 1 (Smaf1) protein in adipocytes.

  2016cited by this paper
• Insulin resistance and white adipose tissue inflammation are uncoupled in energetically challenged Fsp27-deficient mice

  2015cited by this paper
• LocSigDB: a database of protein localization signals

  2015cited by this paper
• 20 years of leptin: leptin at 20: an overview.

  2014cited by this paper
• Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2

  2014cited by this paper
• T-Coffee: a web server for the multiple sequence alignment of protein and RNA sequences using structural information and homology extension

  2011cited by this paper
• Mouse models of inherited lipodystrophy

  2009cited by this paper
• Insulin regulates leptin secretion from 3T3-L1 adipocytes by a PI 3 kinase independent mechanism.

  2008cited by this paper
• PPAR gamma 2 Prevents Lipotoxicity by Controlling Adipose Tissue Expandability and Peripheral Lipid Metabolism

  2007cited by this paper
• Acute and chronic regulation of leptin synthesis, storage, and secretion by insulin and dexamethasone in human adipose tissue.

  2007cited by this paper
• Leptin deficiency unmasks the deleterious effects of impaired peroxisome proliferator-activated receptor gamma function (P465L PPARgamma) in mice.

  2006cited by this paper
• Leptin Deficiency Unmasks the Deleterious Effects of Impaired Peroxisome Proliferator–Activated Receptor γ Function (P465L PPARγ) in Mice

  2006cited by this paper
• An HMM posterior decoder for sequence feature prediction that includes homology information

  2005cited by this paper
• Up-regulation of adipogenin, an adipocyte plasma transmembrane protein, during adipogenesis

  2005cited by this paper
• Cloning, expression, and differentiation-dependent regulation of SMAF1 in adipogenesis.

  2004cited by this paper
• Adipocyte-specific Gene Expression and Adipogenic Steatosis in the Mouse Liver Due to Peroxisome Proliferator-activated Receptor γ1 (PPARγ1) Overexpression*

  2003cited by this paper
• Adipocyte-specific gene expression and adipogenic steatosis in the mouse liver due to peroxisome proliferator-activated receptor gamma1 (PPARgamma1) overexpression.

  2003cited by this paper

• Making a droplet: the adipogenin-seipin complex at work.

  2026cites this paper
• Small proline-rich protein 1A is a novel target of peroxisome proliferator-activated receptor gamma in steatotic liver disease.

  2026cites this paper
• The longevity effects of reduced IGF-1 signaling depend on the stability of the mitochondrial genome

  2025cites this paper
• 14-3-3ζ allows for adipogenesis by modulating chromatin accessibility during the early stages of adipocyte differentiation

  2025cites this paper
• Transcriptome analysis unveils multiple reasons behind delayed and slower deposition of intramuscular fat compared to subcutaneous fat in cattle

  2025cites this paper
• Transcriptional Regulation of the Interferon Alpha‐Inducible Protein‐27 Like 2b by the Peroxisome Proliferator‐Activated Receptor γ in Fatty Liver Disease

  2025cites this paper
• Molecular Insights into Intramuscular Unsaturated Fatty Acid Deposition in Lambs Through Multi-Omics Profiling

  2025cites this paper
• Molecular Genetics and Epigenetics Regulatory Role of miRNAs on Adipogenesis and Intramuscular Fat Development in Beef Cattle: In the Context of Meat Quality.

  2025cites this paper
• Adipogenin promotes the development of lipid droplets by binding a dodecameric seipin complex.

  2025cites this paper
• Comparative histomorphometric and transcriptomic analysis reveals potential genetic determinants of pelage variation between hairy and coarse-woolly sheep

  2025cites this paper
• Five hundred million years of hunger: how animals evolved to survive essential amino acid scarcity

  2025cites this paper
• Adipogenin-seipin, lipid droplet architecture and the expanding metabolic frontier: Implications for metabolic disorders and cancer

  2025cites this paper
• Glycoprotein nonmetastatic melanoma protein B is regulated by hepatic peroxisome proliferator-activated receptor γ in liver steatosis.

  2025cites this paper
• 14-3-3ζ regulates adipogenesis by modulating chromatin accessibility during the early stages of adipocyte differentiation

  2024cites this paper
• Transmembrane and coiled-coil domain family 3 gene is a novel target of hepatic peroxisome proliferator-activated receptor γ in fatty liver disease.

  2024cites this paper
• Transcriptional regulation of adipogenin expression in liver steatosis by hepatic peroxisome proliferator‐activated receptor gamma

  2023cites this paper
• Evaluation of the Genetic Diversity, Population Structure and Selection Signatures of Three Native Chinese Pig Populations

  2023cites this paper
• Screening of Bovine Tissue-Specific Expressed Genes and Identification of Genetic Variation Within an Adipose Tissue-Specific lncRNA Gene

  2022cites this paper
• A mouse model of human mitofusin-2-related lipodystrophy exhibits adipose-specific mitochondrial stress and reduced leptin secretion

  2022cites this paper
• The crosstalk between gut microbiota, intestinal immunological niche and visceral adipose tissue as a new model for the pathogenesis of metabolic and inflammatory diseases: the paradigm of type 2 diabetes mellitus.

  2022cites this paper
• Muscle transcriptome analysis reveal candidate genes and pathways related to fat and lipid metabolism in Yunling cattle

  2021cites this paper
• Expression Profile Analysis of Long Non-coding RNA in OVX Models-Derived BMSCs for Postmenopausal Osteoporosis by RNA Sequencing and Bioinformatics

  2021cites this paper