Toxicity of Chlorpyrifos-Based Pesticide Formulation Termicot® on Non-target Organism (Clarias gariepinus)

P. Abara,C. Madu,M. Nwachukwu,V. Ubah,A. Udebuani,L. Adjeroh

Published 2020 in Unknown venue

ABSTRACT

Chlorpyrifos is a highly toxic organophosphate pesticide used in agriculture. The surface run-offs from the use of this insecticide enter the aquatic ecosystem where it adversely affects fish and other aquatic organisms. This study was carried out to investigate the toxicity of Termicot® in the African cat fish, Clarias gariepinus. Before the 96 h acute toxicity testing, the physicochemical parameters of the water were analyzed. 250 juveniles of C. gariepinus with 2-4 cm length were exposed to varying concentrations of Termicot®. Range finding test was followed by 96 h acute toxicity test. The test fishes were exposed to sub-lethal concentrations representing one quarter and one eight of the LC50 for fifteen days. Blood samples were collected from exposed fish on the 5th, 10th and 15th days and evaluated for the presence of micronuclei. After various trials in the range finding test, it was observed that 2.00 mg/l killed 75% of the test fish species while 0.50mg/l killed 25%. The LC50 determined was 1.2811 mg/l. The frequency of induction of micronuclei significantly increased (P < 0.05) with increase of concentration and decreased with increase of the exposure period. It is recommended that Termicot® should be used where it is the only option, and it should be in controlled concentration.

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