RNAi of the N‐glycosylation‐related genes confirms their importance in insect development and α‐1,6‐fucosyltransferase plays a role in the ecdysis event for the hemimetabolous pest insect Nilaparvata lugens

Recently N‐glycosylation was found to be required for the postembryonic development and metamorphosis of the holometabolous beetle Tribolium castaneum. However, the role of N‐glycosylation in the development of hemimetabolous insects is unknown. To further elucidate the role of N‐glycosylation in the development of insects, a functional characterization of the N‐glycosylation‐related genes (NGRGs) was performed in a model insect for hemimetabolous development, namely the brown planthopper Nilaparvata lugens. In this project, we report the effects of RNAi‐mediated silencing of 15 NGRGs on the postembryonic development of N. lugens. Two major observations were made. First, interruption of the early steps of N‐glycan processing led to a lethal phenotype during the transition from nymph to adult as was observed in T. castaneum. Second, we report here on an essential function for the α‐1,6‐fucosyl transferase in the ecdysis event of N. lugens between nymphal instars, since gene‐silencing by RNAi led to failure of ecdysis and subsequent mortality of the treated insect.

• Publication year

  2021

• Venue

  Insect Science

• Publication date

  2021-04-16

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1111/1744-7917.12920PMID 33860636
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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