MicroRNA expression in formalin-fixed paraffin embedded tissue using real time quantitative PCR: the strengths and pitfalls

Jeroen R. Dijkstra,L. Mekenkamp,Steven Teerenstra,I. D. Krijger,Iris D. Nagtegaal

Published 2012 in Journal of Cellular and Molecular Medicine

ABSTRACT

•  Introduction •  RNA isolation from formalin‐fixed paraffin‐embedded (FFPE) tissue •  RNA quality control •  cDNA synthesis •  Real Time Quantitative PCR •  Reference genes •  Data analysis •  Conclusions

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