Identification of a Novel Set of Genes Regulated by a Unique Liver X Receptor-α-mediated Transcription Mechanism*

We have reported previously that liver X receptor-α (LXRα) can mediate a novel cAMP-dependent increase in renin and c-myc gene transcription by binding as a monomer to a unique regulatory element termed the cAMP-negative response element (CNRE). To determine whether this novel action of LXRα has global implications on gene regulation, we employed expression profiling to identify other genes regulated by this unique mechanism. Here we report the existence of a set of known and unknown transcripts regulated in parallel with renin. Querying the Celera Mouse Genome Assembly revealed that a majority of these genes contained the consensus CNRE. We have confirmed the functionality of these CNREs by competition for LXRα binding via electrophoretic mobility shift assays (EMSA) and by the use of CNRE decoy molecules documenting the abolishment of the cAMP-mediated gene induction. Taken together, these results demonstrate that the interaction between cAMP-activated LXRα and the CNRE enhancer element is responsible for widespread changes in gene expression and identify a set of LXRα/cAMP-regulated genes that may have important biological implications.

• Publication year

  2003

• Venue

  Journal of Biological Chemistry

• Publication date

  2003-01-27

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1074/JBC.M208644200PMID 12551904
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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