A novel transaminase, (R)-amine:pyruvate aminotransferase, from Arthrobacter sp. KNK168 (FERM BP-5228): purification, characterization, and gene cloning

No abstract is available for this paper.

• Publication year

  2012

• Venue

  Applied Microbiology and Biotechnology

• Publication date

  2012-02-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1007/s00253-011-3580-0PMID 22002066
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Rational assignment of key motifs for function guides in silico enzyme identification.

  2010cited by this paper
• Application of a Multiple‐Enzyme System for Chiral Alcohol Production

  2010cited by this paper
• Identification of ω-aminotransferase from Caulobacter crescentus and site-directed mutagenesis to broaden substrate specificity.

  2008cited by this paper
• Asymmetric Synthesis of Optically Pure Pharmacologically Relevant Amines Employing ω‐Transaminases

  2008cited by this paper
• Preparation of (R)-Amines from Racemic Amines with an (S)-Amine Transaminase from Bacillus megaterium

  2008influential reference
• Substrate spectrum of ω-transaminase from Chromobacterium violaceum DSM30191 and its potential for biocatalysis

  2007influential reference
• Microbial synthesis of chiral amines by (R)-specific transamination with Arthrobacter sp. KNK168

  2005cited by this paper
• Kinetic resolution of (R,S)‐sec‐butylamine using omega‐transaminase from Vibrio fluvialis JS17 under reduced pressure

  2004influential reference
• omega-Amino acid:pyruvate transaminase from Alcaligenes denitrificans Y2k-2: a new catalyst for kinetic resolution of beta-amino acids and amines.

  2004cited by this paper
• ω-Amino Acid:Pyruvate Transaminase from Alcaligenes denitrificans Y2k-2: a New Catalyst for Kinetic Resolution of β-Amino Acids and Amines

  2004influential reference
• Novel bioreduction system for the production of chiral alcohols

  2003cited by this paper
• Purification, characterization, and molecular cloning of a novel amine:pyruvate transaminase from Vibrio fluvialis JS17

  2003cited by this paper
• Microbial synthesis of (R)- and (S)-3,4-dimethoxyamphetamines through stereoselective transamination

  2003influential reference
• Asymmetric Catalysis on Industrial Scale

  2003cited by this paper
• Comparison of the ω-Transaminases from Different Microorganisms and Application to Production of Chiral Amines

  2001influential reference
• Production of thermotolerant N-carbamyl-D-amino acid amidohydrolase by recombinant Escherichia coli.

  1999influential reference
• Asymmetric synthesis of chiral amines with ω‐transaminase

  1999cited by this paper
• Asymmetric synthesis of chiral amines with omega-transaminase.

  1999cited by this paper
• Structure, evolution and action of vitamin B6-dependent enzymes.

  1998cited by this paper
• Kinetic resolution of alpha-methylbenzylamine with omicron-transaminase screened from soil microorganisms: application of a biphasic system to overcome product inhibition.

  1997cited by this paper
• Kinetic resolution of α-methylbenzylamine with ο-transaminase screened from soil microorganisms: Application of a biphasic system to overcome product inhibition

  1997influential reference
• The Use of Aminotransferases for the Production of Chiral Amino Acids and Amines

  1993influential reference
• Fluorimetric determination of secondary amino acids by 7-fluoro-4-nitrobenzo-2-oxa-1,3-diazole

  1981cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper

• D-Amino Acid Transaminases: Structural Diversity, Catalytic Properties, and Potential Applications

  2026cites this paper
• AlphaFold-Guided Semi-Rational Engineering of an (R)-Amine Transaminase for Green Synthesis of Chiral Amines

  2025cites this paper
• Reductive amination: Methods for cell-free and whole-cell biocatalysis.

  2025cites this paper
• Integration of Computational Tools for Rational Design of (R)-ω-Transaminases with Enhanced Asymmetric Catalysis

  2025cites this paper
• Characterization and substrate specificity study of the novel (R)-amine transaminase MagAT

  2025influential citation
• Identification, characterization and application of M16AT, a new organic solvent–tolerant (R)–enantio–selective type IV amine transaminase from Mycobacterium sp. ACS1612

  2024cites this paper
• ω-transaminase-catalyzed synthesis of (R)-2-(1-aminoethyl)-4-fluorophenol, a chiral intermediate of novel anti-tumor drugs.

  2024cites this paper
• Reprogramming biocatalytic futile cycles through computational engineering of stereochemical promiscuity to create an amine racemase

  2024cites this paper
• From Structure to Function: Analysis of the First Monomeric Pyridoxal-5′-Phosphate-Dependent Transaminase from the Bacterium Desulfobacula toluolica

  2024cites this paper
• The Promising Role of Amine Transaminase Cascades in the Synthesis of Non-Canonical Amino Acids

  2024cites this paper
• Identification of efficient amine transaminase and applicability in dual transaminases cascade for synthesis of L-phosphinothricin.

  2024cites this paper
• Engineering an (R)-selective transaminase for asymmetric synthesis of (R)-3-aminobutanol.

  2024cites this paper
• Biosynthesis of Furfurylamines in Batch and Continuous Flow by Immobilized Amine Transaminases

  2023cites this paper
• Activity of endophytic fungi in enantioselective biotransformation of chiral amines: New approach for solid-state fermentation

  2023cites this paper
• Identification and characterization of a novel thermostable transaminase (TATP) from Thermorudis peleae

  2023cites this paper
• Structural Basis for Allostery in PLP-dependent Enzymes

  2022cites this paper
• Structure and Cooperativity in Substrate-Enzyme Interactions: Perspectives on Enzyme Engineering and Inhibitor Design.

  2022cites this paper
• Engineering Novel (R)-Selective Transaminase for Efficient Symmetric Synthesis of d-Alanine

  2022cites this paper
• Non-Canonical Amino Acid-Based Engineering of (R)-Amine Transaminase

  2022cites this paper
• Redesign of (R)-Omega-Transaminase and Its Application for Synthesizing Amino Acids with Bulky Side Chain

  2021cites this paper
• Identification, Characterization, and Site-Specific Mutagenesis of a Thermostable ω-Transaminase from Chloroflexi bacterium

  2021cites this paper
• Identi�cation, Characterization and Site-Saturation Mutagenesis of a Thermostable ω -Transaminase From Chloro�exi Bacterium

  2021cites this paper
• Probing the role of the residues in the active site of the transaminase from Thermobaculum terrenum

  2021cites this paper
• One pot cascade synthesis of L-2-aminobutyric acid employing ω-transaminase from Paracoccus pantotrophus

  2021cites this paper
• Identification, Characterization and Site-Saturation Mutagenesis of a Thermostable ω-Transaminase From Chloroflexi Bacterium

  2021cites this paper
• Structural and Functional Analysis of the Only Two Pyridoxal 5′-Phosphate-Dependent Fold Type IV Transaminases in Bacillus altitudinis W3

  2020cites this paper
• Transaminases for industrial biocatalysis: novel enzyme discovery

  2020cites this paper
• Structural insight into the substrate specificity of PLP fold type IV transaminases

  2020cites this paper
• Continuous Flow Bioamination of Ketones in Organic Solvents at Controlled Water Activity using Immobilized ω‐Transaminases

  2020cites this paper
• Parallel Interconnected Kinetic Asymmetric Transformation (PIKAT) with an Immobilized ω-Transaminase in Neat Organic Solvent

  2020cites this paper
• Combinatorial Mutation Analysis of ω‐Transaminase to Create an Engineered Variant Capable of Asymmetric Amination of Isobutyrophenone

  2019cites this paper
• Construction of stabilized (R)-selective amine transaminase from Aspergillus terreus by consensus mutagenesis.

  2019cites this paper
• Functional characterization of PLP fold type IV transaminase with a mixed type of activity from Haliangium ochraceum.

  2019cites this paper
• Stereoselective Synthesis of 1‐Arylpropan‐2‐amines from Allylbenzenes through a Wacker‐Tsuji Oxidation‐Biotransamination Sequential Process

  2019cites this paper
• Rapid and Quantitative Profiling of Substrate Specificity of ω‐Transaminases for Ketones

  2019cites this paper
• Methoxamine Synthesis in a Biocatalytic 1-Pot 2-Step Cascade Approach

  2019cites this paper
• PLP-dependent enzymes as important biocatalysts for the pharmaceutical, chemical and food industries: a structural and mechanistic perspective

  2019cites this paper
• Characterization of a novel thermostable (S)-amine-transaminase from an Antarctic moderately-thermophilic bacterium Albidovulum sp. SLM16.

  2019cites this paper
• Biochemical and structural insights into PLP fold type IV transaminase from Thermobaculum terrenum.

  2019cites this paper
• Isopropylamine as Amine Donor in Transaminase‐Catalyzed Reactions: Better Acceptance through Reaction and Enzyme Engineering

  2018cites this paper
• Highly efficient production of chiral amines in batch and continuous flow by immobilized ω-transaminases on controlled porosity glass metal-ion affinity carrier

  2018influential citation
• Crystal structure of branched-chain amino acid aminotransferase from Thermobaculum terrenum in PMP-form

  2018cites this paper
• Identification of branched-chain amino acid aminotransferases active towards (R)-(+)-1-phenylethylamine among PLP fold type IV transaminases.

  2018cites this paper
• The ω‐transaminase engineering database (oTAED): A navigation tool in protein sequence and structure space

  2018cites this paper
• Characterization of ELP-fused ω-Transaminase and Its Application for the Biosynthesis of β-Amino Acid

  2018cites this paper
• Identification of (R)-selective ω-aminotransferases by exploring evolutionary sequence space.

  2018cites this paper
• Immobilization of R-ω-transaminase on MnO2 nanorods for catalyzing the conversion of (R)-1-phenylethylamine.

  2017cites this paper
• Discovery, Engineering, and Synthetic Application of Transaminase Biocatalysts

  2017cites this paper
• Characterization of a (R)-selective amine transaminase from Fusarium oxysporum

  2017cites this paper
• A Novel highly thermostable branched-chain amino acid aminotransferase from the crenarchaeon Vulcanisaeta moutnovskia.

  2017cites this paper
• Properties of bacterial and archaeal branched-chain amino acid aminotransferases

  2017cites this paper
• Identification, expression and characterization of an R-ω-transaminase from Capronia semiimmersa

  2017influential citation
• Transaminase biocatalysis: optimization and application

  2017cites this paper
• [Deletion of a dynamic surface loop improves thermostability of (R)-selective amine transaminase from Aspergillus terreus].

  2017cites this paper
• Genetically-modified R-ω-transaminase: purification and self-assembly facilitating interaction with substrate droplets

  2016cites this paper
• Discovery and structural characterisation of new fold type IV-transaminases exemplify the diversity of this enzyme fold

  2016cites this paper
• Characterization of (R)-selective amine transaminases identified by in silico motif sequence blast

  2015cites this paper
• Kinetic resolution of amines by (R)-selective omega-transaminase from Mycobacterium vanbaalenii

  2015cites this paper
• Active-Site Engineering of ω-Transaminase for Production of Unnatural Amino Acids Carrying a Side Chain Bulkier than an Ethyl Substituent

  2015cites this paper
• A new target region for changing the substrate specificity of amine transaminases

  2015cites this paper
• Biocatalytic cascade reactions for asymmetric synthesis of aliphatic amino acids in a biphasic reaction system

  2015cites this paper
• Deracemization of amino acids by coupling transaminases of opposite stereoselectivity

  2014cites this paper
• Multi-enzymatic Systems for the Production of Chiral Compounds

  2014cites this paper
• Crystal Structure of an (R)-Selective ω-Transaminase from Aspergillus terreus

  2014cites this paper
• Biocatalytic potential of vanillin aminotransferase from Capsicum chinense

  2014cites this paper
• Substrate profile of an ω-transaminase from Burkholderia vietnamiensis and its potential for the production of optically pure amines and unnatural amino acids

  2014cites this paper
• One‐Pot Production of Enantiopure Alkylamines and Arylalkylamines of Opposite Chirality Catalyzed by ω‐Transaminase

  2013cites this paper
• Biocatalytic Asymmetric Synthesis of Unnatural Amino Acids through the Cascade Transfer of Amino Groups from Primary Amines onto Keto Acids

  2013cites this paper
• The fundamentals and fun of biocatalysis

  2013cites this paper
• Reusable ω-transaminase sol–gel catalyst for the preparation of amine enantiomers

  2013cites this paper
• ω-Transaminase-catalyzed asymmetric synthesis of unnatural amino acids using isopropylamine as an amino donor.

  2013cites this paper