Transport, resealing, and re-poration dynamics of two-pulse electroporation-mediated molecular delivery.

No abstract is available for this paper.

• Publication year

  2015

• Venue

  Biochimica et Biophysica Acta

• Publication date

  2015-08-01

• Fields of study

  Medicine, Materials Science, Chemistry, Engineering

• Identifiers
  DOI 10.1016/j.bbamem.2015.04.007PMID 25911207
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• High-yield nontoxic gene transfer through conjugation of the CM₁₈-Tat₁₁ chimeric peptide with nanosecond electric pulses.

  2014cited by this paper
• Scaling relationship and optimization of double-pulse electroporation.

  2014cited by this paper
• Quantification of propidium iodide delivery using millisecond electric pulses: experiments.

  2013cited by this paper
• Effect of different parameters used for in vitro gene electrotransfer on gene expression efficiency, cell viability and visualization of plasmid DNA at the membrane level

  2013cited by this paper
• Electroporation Based Drug Delivery and Its Applications

  2013cited by this paper
• Thresholds for Phosphatidylserine Externalization in Chinese Hamster Ovarian Cells following Exposure to Nanosecond Pulsed Electrical Fields (nsPEF)

  2013cited by this paper
• Drug delivery by electropulsation: Recent developments in oncology.

  2012cited by this paper
• Size-controlled nanopores in lipid membranes with stabilizing electric fields.

  2012cited by this paper
• Size of the pores created by an electric pulse: microsecond vs millisecond pulses.

  2012cited by this paper
• The effect of extracellular conductivity on electroporation-mediated molecular delivery.

  2012cited by this paper
• A stochastic model for DNA translocation through an electropore.

  2012cited by this paper
• Numerical simulation of molecular uptake via electroporation.

  2011cited by this paper
• Electroporation based gene therapy — From the bench to the bedside

  2011cited by this paper
• The Role of Electrophoresis in Gene Electrotransfer

  2010influential reference
• Numerical study of the electroporation pulse shape effect on molecular uptake of biological cells

  2010cited by this paper
• Life Cycle of an Electropore: Field-Dependent and Field-Independent Steps in Pore Creation and Annihilation

  2010cited by this paper
• Combined Pulse Electroporation – A Novel Strategy for Highly Efficient Transfection of Human and Mouse Cells

  2010cited by this paper
• Transmembrane potential measurements on plant cells using the voltage-sensitive dye ANNINE-6

  2010cited by this paper
• Electro‐mediated gene transfer and expression are controlled by the life‐time of DNA/membrane complex formation

  2010cited by this paper
• What is (Still not) Known of the Mechanism by Which Electroporation Mediates Gene Transfer and Expression in Cells and Tissues

  2009cited by this paper
• Mechanisms involved in gene electrotransfer using high- and low-voltage pulses--an in vitro study.

  2009cited by this paper
• Efficient Transfection of Endothelial Cells by a Double-Pulse Electroporation Method

  2009cited by this paper
• Electroporating Fields Target Oxidatively Damaged Areas in the Cell Membrane

  2009cited by this paper
• Nonlinear current response of micro electroporation and resealing dynamics for human cancer cells.

  2008cited by this paper
• Quantification of electroporative uptake kinetics and electric field heterogeneity effects in cells.

  2008cited by this paper
• Theoretical and experimental analysis of conductivity, ion diffusion and molecular transport during cell electroporation--relation between short-lived and long-lived pores.

  2008cited by this paper
• Efficiency of High- and Low-Voltage Pulse Combinations for Gene Electrotransfer in Muscle, Liver, Tumor, and Skin

  2008cited by this paper
• Mechanistic Analysis of Electroporation-Induced Cellular Uptake of Macromolecules

  2008cited by this paper
• Low voltage pulses can induce apoptosis.

  2008cited by this paper
• Kinetics of transmembrane transport of small molecules into electropermeabilized cells.

  2008cited by this paper
• Physiological Effects of High- and Low-Voltage Pulse Combinations for Gene Electrotransfer in Muscle

  2008cited by this paper
• Single-cell electroporation arrays with real-time monitoring and feedback control.

  2007cited by this paper
• Real time electroporation control for accurate and safe in vivo non-viral gene therapy.

  2007cited by this paper
• Modeling electroporation in a single cell.

  2007cited by this paper
• Optimization of cutaneous electrically mediated plasmid DNA delivery using novel electrode

  2007cited by this paper
• Electrochemotherapy: Aspects of Preclinical Development and Early Clinical Experience

  2007cited by this paper
• Nonlinear current-voltage relationship of the plasma membrane of single CHO cells.

  2007cited by this paper
• A method for genetic modification of human embryonic stem cells using electroporation

  2007cited by this paper
• Using a micro electroporation chip to determine the optimal physical parameters in the uptake of biomolecules in HeLa cells.

  2007cited by this paper
• RNA loading of leukemic antigens into cord blood-derived dendritic cells for immunotherapy.

  2006cited by this paper
• High-efficiency transfection of primary human and mouse T lymphocytes using RNA electroporation.

  2006cited by this paper
• Cell membrane fluidity related to electroporation and resealing

  2006cited by this paper
• Enhancement of biomolecule transport by electroporation: a review of theory and practical application to transformation of Corynebacterium glutamicum.

  2006cited by this paper
• Effects of extracellular calcium on cell membrane resealing in sonoporation.

  2006cited by this paper
• Electric pulse-mediated gene delivery to various animal tissues.

  2005cited by this paper
• Electrophoretic Component of Electric Pulses Determines the Efficacy of In Vivo DNA Electrotransfer

  2005cited by this paper
• Study of mechanisms of electric field-induced DNA transfection

  2005cited by this paper
• DNA electrotransfer into the skin using a combination of one high- and one low-voltage pulse.

  2005cited by this paper
• DNA electrotransfer: its principles and an updated review of its therapeutic applications

  2004cited by this paper
• In vitro and in vivo electric field-mediated permeabilization, gene transfer, and expression.

  2004cited by this paper
• Effect of electric field vectoriality on electrically mediated gene delivery in mammalian cells.

  2004cited by this paper
• Role of pulse shape in cell membrane electropermeabilization.

  2003cited by this paper
• Electroporation: theory and methods, perspectives for drug delivery, gene therapy and research.

  2003cited by this paper
• Direct visualization at the single-cell level of electrically mediated gene delivery

  2002cited by this paper
• The effect of pulse repetition frequency on the uptake into electropermeabilized cells in vitro with possible applications in electrochemotherapy.

  2002cited by this paper
• Mechanisms of in vivo DNA electrotransfer: respective contributions of cell electropermeabilization and DNA electrophoresis.

  2002cited by this paper
• Recent biotechnological developments of electropulsation. A prospective review.

  2002cited by this paper
• Theoretical analysis of localized heating in human skin subjected to high voltage pulses.

  2002cited by this paper
• The influence of medium conductivity on electropermeabilization and survival of cells in vitro.

  2001cited by this paper
• Quantitative study of electroporation-mediated molecular uptake and cell viability.

  2001cited by this paper
• Intracellular effect of ultrashort electrical pulses

  2001cited by this paper
• A validated model of in vivo electric field distribution in tissues for electrochemotherapy and for DNA electrotransfer for gene therapy.

  2000cited by this paper
• Importance of association between permeabilization and electrophoretic forces for intramuscular DNA electrotransfer.

  2000influential reference
• Principles of membrane electroporation and transport of macromolecules.

  2000cited by this paper
• Modeling electroporation in a single cell. I. Effects Of field strength and rest potential.

  1999cited by this paper
• Electropermeabilization of cell membranes.

  1999cited by this paper
• High-efficiency gene transfer into skeletal muscle mediated by electric pulses.

  1999cited by this paper
• Electropermeabilization of mammalian cells to macromolecules: control by pulse duration.

  1998cited by this paper
• Direct observation in the millisecond time range of fluorescent molecule asymmetrical interaction with the electropermeabilized cell membrane.

  1997cited by this paper
• Pore disappearance in a cell after electroporation: theoretical simulation and comparison with experiments.

  1997cited by this paper
• Electroporation: basic principles, practical considerations and applications in molecular biology

  1997cited by this paper
• Animal cell electroporation and electrofusion protocols

  1995cited by this paper
• Control by electrical parameters of short- and long-term cell death resulting from electropermeabilization of Chinese hamster ovary cells.

  1995cited by this paper
• Introduction of macromolecules into living Dictyostelium cells by electroporation.

  1995cited by this paper
• Control by pulse parameters of electric field-mediated gene transfer in mammalian cells.

  1994influential reference
• Quantitative study of molecular transport due to electroporation: uptake of bovine serum albumin by erythrocyte ghosts.

  1994cited by this paper
• A quantitative study of electroporation showing a plateau in net molecular transport.

  1993cited by this paper
• Time courses of cell electroporation as revealed by submicrosecond imaging of transmembrane potential.

  1993cited by this paper
• An experimental evaluation of the critical potential difference inducing cell membrane electropermeabilization.

  1993cited by this paper
• Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry.

  1992cited by this paper
• Electroporation and electrophoretic DNA transfer into cells. The effect of DNA interaction with electropores.

  1992cited by this paper
• Membrane electroporation and direct gene transfer

  1992cited by this paper
• Electroporation: a unified, quantitative theory of reversible electrical breakdown and mechanical rupture in artificial planar bilayer membranes☆

  1991cited by this paper
• Electroporation by using bipolar oscillating electric field: an improved method for DNA transfection of NIH 3T3 cells.

  1991cited by this paper
• Membrane conductance of an electroporated cell analyzed by submicrosecond imaging of transmembrane potential.

  1991cited by this paper
• Study of mechanisms of electric field-induced DNA transfection. I. DNA entry by surface binding and diffusion through membrane pores.

  1990cited by this paper
• Schwan equation and transmembrane potential induced by alternating electric field.

  1990cited by this paper
• Electroporation of cell membrane visualized under a pulsed-laser fluorescence microscope.

  1988cited by this paper
• The electrical breakdown of cell and lipid membranes: the similarity of phenomenologies.

  1987cited by this paper
• Gene transfer into mouse lyoma cells by electroporation in high electric fields.

  1982cited by this paper

• Efficacy of Plasmid DNA Delivery into Mice by Intradermal Injections Alone and Facilitated by Sonoporation or Electroporation

  2026cites this paper
• Assessment of cell electroporation on nanosecond timescales based on electroporation current

  2025cites this paper
• The Role of the CLIC/GEEC Endocytic Pathway for Mechanophysical Transfection of DNA

  2025cites this paper
• Optimizing electroporation via pulse modulation: a molecular dynamics study

  2025cites this paper
• Molecular dynamics of electroporation and quantitative analysis of molecular transport

  2025cites this paper
• Numerical modeling of giant pore formation in vesicles under msPEF-induced electroporation: Role of charging time and waveform.

  2025cites this paper
• An Electroporation-Mediated Intracellular Delivery Model With Coupled Pore Currents and Solute Transport

  2024cites this paper
• Quantitative analysis of electroporation-mediated intracellular delivery via bioorthogonal luminescent reaction

  2024cites this paper
• Regulating the Distribution and Accumulation of Charged Molecules by Progressive Electroporation for Improved Intracellular Delivery.

  2024cites this paper
• Giant pore formation in vesicles under msPEF-induced electroporation: role of charging time and waveform

  2024cites this paper
• A molecular dynamics study of phospholipid membrane electroporation induced by bipolar pulses with different intervals.

  2023cites this paper
• Recent advances in microfluidic-based electroporation techniques for cell membranes.

  2022cites this paper
• Beneath the Skin: A Review of Current Trends and Future Prospects of Transdermal Drug Delivery Systems

  2022cites this paper
• In-Cell Structural Biology by NMR: The Benefits of the Atomic Scale.

  2022cites this paper
• The Phenomenon of Electroporation

  2022cites this paper
• An Overview Focusing on Food Liposomes and Their Stability to Electric Fields

  2022cites this paper
• How to alleviate cardiac injury from electric shocks at the cellular level

  2022cites this paper
• Moderate electric field-assisted hydro-distillation of thyme essential oil: characterization of microstructural changes

  2022cites this paper
• Scanning electrochemical microscopy based irreversible destruction of living cells.

  2022cites this paper
• Molecular dynamics study of phospholipid membrane electroporation induced by bipolar pulses with different intervals

  2022cites this paper
• Method Development Progress in Genetic Engineering of Thraustochytrids

  2021cites this paper
• High‐Throughput and Dosage‐Controlled Intracellular Delivery of Large Cargos by an Acoustic‐Electric Micro‐Vortices Platform

  2021cites this paper
• Ultralong-Time Recovery and Low-Voltage Electroporation for Biological Cell Monitoring Enabled by a Microsized Multipulse Framework

  2021cites this paper
• Delivering defined amounts of purified protein with high precision into living cells

  2021cites this paper
• Single-cell electroporation with high-frequency nanosecond pulse bursts: Simulation considering the irreversible electroporation effect and experimental validation.

  2021cites this paper
• Cell death due to electroporation - A review.

  2021cites this paper
• Enhanced transmembrane transport of reactive oxygen species by electroporation effect of plasma

  2021cites this paper
• Mechanistic studies of gene delivery into mammalian cells by electrical short-circuiting via an aqueous droplet in dielectric oil

  2020cites this paper
• Detection of intracellular biomarkers in viable cells using millisecond pulsed electric fields.

  2020cites this paper
• The effects of electroporation buffer composition on cell viability and electro-transfection efficiency

  2020cites this paper
• Utilizing a Franz cell device for the electroporation of adherent cells with nanoporous alumina substrates

  2020cites this paper
• Extracellular-Ca2+-Induced Decrease in Small Molecule Electrotransfer Efficiency: Comparison between Microsecond and Nanosecond Electric Pulses

  2020cites this paper
• Impedance Analysis of Tissues in nsPEF Treatment for Cancer Therapy

  2020cites this paper
• Iontophoresis, magnetophoresis, and electroporation

  2020cites this paper
• Nanosecond pulses targeting intracellular ablation increase destruction of tumor cells with irregular morphology.

  2019cites this paper
• Human CAR NK Cells: A New Non-viral Method Allowing High Efficient Transfection and Strong Tumor Cell Killing

  2019cites this paper
• In vitro electroporation detection methods - An overview.

  2018cites this paper
• Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.

  2018cites this paper
• On-Demand Intracellular Delivery of Single Particles in Single Cells by 3D Hollow Nanoelectrodes

  2018cites this paper
• Different permeabilization patterns of splenocytes and thymocytes to combination of pulsed electric and magnetic field treatments.

  2018cites this paper
• Quantitative In Vitro Characterization of Membrane Permeability for Electroporated Mammalian Cells

  2018cites this paper
• Characterization of Cell Membrane Permeability In Vitro Part II: Computational Model of Electroporation-Mediated Membrane Transport*

  2018cites this paper
• Analysis of Dynamic Processes in Single-Cell Electroporation and Their Effects on Parameter Selection Based on the Finite-Element Model

  2017cites this paper
• Membrane Permeabilization Lifetime in Experiments

  2017cites this paper
• Continuous-flow, electrically-triggered, single cell-level electroporation

  2017cites this paper
• Irreversible electroporation ablation area enhanced by synergistic high- and low-voltage pulses

  2017cites this paper
• Perspectives on Transdermal Electroporation

  2016influential citation
• Mechanoporation of living cells for delivery of macromolecules using nanoneedle array.

  2016cites this paper
• Multi-parameter analysis in single-cell electroporation based on the finite element model

  2016cites this paper
• Hydrodynamically controlled cell rotation in an electroporation microchip to circumferentially deliver molecules into single cells

  2016cites this paper
• Tutorial: Electroporation of cells in complex materials and tissue

  2016cites this paper
• Dependence of Electroporation Detection Threshold on Cell Radius: An Explanation to Observations Non Compatible with Schwan’s Equation Model

  2016cites this paper
• Post-pulse addition of trans-cyclohexane-1,2-diol improves electrotransfer mediated gene expression in mammalian cells

  2016cites this paper
• Cell Electrosensitization Exists Only in Certain Electroporation Buffers

  2016cites this paper
• Measurement of Transient Permeability of Sp2/0 Myeloma Cells: Flow Cytometric Study

  2016cites this paper
• Transdermal delivery of heparin: Physical enhancement techniques.

  2015cites this paper