Comparative genomics of Helicobacter pylori isolates recovered from ulcer disease patients in England

BackgroundGenomic diversity of H. pylori from many different human populations is largely unknown. We compared genomes of 65 H. pylori strains from Nottingham, England. Molecular analysis was carried out to identify rearrangements within and outside the cag-pathogenicity-island (cag PAI) and DNA sequence divergence in candidate genes. Phylogenetic analysis was carried out based on various high-resolution genotyping techniques.ResultsAnalyses of virulence genes (cag T, cag E, cag A, vac A, ice A, oip A and bab B) revealed that H. pylori strains from England are genetically distinct from strains obtained from other countries. The toxigenic vac A s1m1 genotype was found to be less common and the plasticity region cluster was found to be disrupted in all the isolates. English isolates showed a predominance of ice A1 alleles and a functional proinflammatory oip A gene. The English H. pylori gene pool revealed several Asian/oriental features. This included the predominance of cag A – glr (cag A right junction) motif types III and II (up to 42%), presence of vac A m1c alleles and phylogenetic affinity towards East Asian / Amerindian gene pools based on fluorescent amplified fragment length polymorphism (FAFLP) analysis and glm M sequence analysis.ConclusionOverall, our results demonstrated genetic affinities of H. pylori in England with both European and the Asian gene pools and some distinctive genetic features of virulence genes that may have evolved in this important European population.

• Publication year

  2005

• Venue

  BMC Microbiology

• Publication date

  2005-05-25

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1186/1471-2180-5-32PMID 15916705PMCID 1180443
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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• Oncogenic mechanisms of the Helicobacter pylori CagA protein

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• Distinguishing human ethnic groups by means of sequences from Helicobacter pylori: lessons from Ladakh.

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• The cag Pathogenicity Island of Helicobacter pylori Is Disrupted in the Majority of Patient Isolates from Different Human Populations

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• Frontal and stealth attack strategies in microbial pathogenesis

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• Novel activities of the Helicobacter pylori vacuolating cytotoxin: from epithelial cells towards the immune system.

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• Implications of Molecular Genotyping of Helicobacter pylori Isolates from Different Human Populations by Genomic Fingerprinting of Enterobacterial Repetitive Intergenic Consensus Regions for Strain Identification and Geographic Evolution

  2004influential reference
• Helicobacter pylori antibiotic resistance patterns and genotypes in adult dyspeptic patients from a regional population in North Wales.

  2004cited by this paper
• Modification of Helicobacter pylori outer membrane protein expression during experimental infection of rhesus macaques

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• Cluster of Type IV Secretion Genes in Helicobacter pylori's Plasticity Zone

  2003cited by this paper
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  2003cited by this paper
• A novel sheathed surface organelle of the Helicobacter pylori cag type IV secretion system

  2003influential reference
• Helicobacter pylori babA2, cagA, and s1 vacA genes work synergistically in causing intestinal metaplasia

  2003influential reference
• Genomic analysis of Helicobacter pylori from Andhra Pradesh, South India: Molecular evidence for three major genetic clusters

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• Fine-Structure Molecular Typing of Irish Helicobacter pylori Isolates and Their Genetic Relatedness to Strains from Four Different Continents

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• Correlation between cag Pathogenicity Island Composition and Helicobacter pylori-Associated Gastroduodenal Disease

  2003influential reference
• Prevalence of Helicobacter pylori vacA, cagA and iceA genotypes in Nigerian patients with duodenal ulcer disease.

  2002cited by this paper
• Helicobacter pylori and gastrointestinal tract adenocarcinomas

  2002cited by this paper
• Surveillance and clinical relevance of vacA genotypes of Helicobacter pylori infecting dyspeptic patients in mid-Essex.

  2002cited by this paper
• Polymorphisms of Helicobacter pylori HP0638 Reflect Geographic Origin and Correlate with cagA Status

  2002influential reference
• The Helicobacter pylori Blood Group Antigen-Binding Adhesin Facilitates Bacterial Colonization and Augments a Nonspecific Immune Response1

  2002cited by this paper
• East Asian genotypes of Helicobacter pylori strains in Amerindians provide evidence for its ancient human carriage

  2002cited by this paper
• H. pylori and gastric cancer: the Asian enigma

  2002cited by this paper
• The burden of Helicobacter pylori infection in England and Wales

  2002cited by this paper
• Characterization of RNase P from Thermotoga maritima.

  2001cited by this paper
• Promoters of the CATG-Specific Methyltransferase Gene hpyIM Differ between iceA1 andiceA2 Helicobacter pylori Strains

  2001cited by this paper
• Strain-specific genes of Helicobacter pylori: distribution, function and dynamics.

  2001cited by this paper
• Allelic Variation within Helicobacter pylori babA and babB

  2001cited by this paper
• cagA-Positive Helicobacter pyloriPopulations in China and The Netherlands Are Distinct

  2000cited by this paper
• Differences in Genotypes of Helicobacter pylori from Different Human Populations

  2000influential reference
• Distinctiveness of Genotypes of Helicobacter pylori in Calcutta, India

  2000cited by this paper
• Distribution of Open Reading Frames of Plasticity Region of Strain J99 in Helicobacter pylori Strains Isolated from Gastric Carcinoma and Gastritis Patients in Costa Rica

  2000cited by this paper
• Geographic distribution of vacA allelic types of Helicobacter pylori.

  1999cited by this paper
• Structure of cag pathogenicity island in Japanese Helicobacter pyloriisolates

  1999cited by this paper
• Helicobacter pylori virulence and genetic geography.

  1999cited by this paper
• cagA-Positive Helicobacter pylori Populations in China and The Netherlands Are Distinct

  1998influential reference
• Clinical relevance of the cagA, vacA, and iceA status of Helicobacter pylori.

  1998cited by this paper
• Clinical outcome after infection with Helicobacter pylori does not appear to be reliably predicted by the presence of any of the genes of the cag pathogenicity island

  1998cited by this paper
• Detection and typing of the virulence determinants cagA and vacA of Helicobacter pylori directly from biopsy DNA: are in vitro strains representative of in vivo strains?

  1998cited by this paper
• H. pylori and cagA: Relationships with Gastric Cancer, Duodenal Ulcer, and Reflux Esophagitis and Its Complications

  1998cited by this paper
• Helicobacter pylori and gastroesophageal reflux disease: the bug may not be all bad

  1998cited by this paper
• Clinical and pathological importance of heterogeneity in vacA, the vacuolating cytotoxin gene of Helicobacter pylori.

  1997cited by this paper
• Risk for gastric cancer in people with CagA positive or CagA negative Helicobacter pylori infection.

  1997cited by this paper
• Unravelling the pathogenic role of Helicobacter pylori in peptic ulcer: potential new therapies and vaccines.

  1994cited by this paper
• Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes.

  1991cited by this paper

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• Considering the clarithromycin resistance prevalence among Helicobacter pylori strains isolated from patients with Gastric complication

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  2024influential citation
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  2023cites this paper
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  2022cites this paper
• Presence of cagPAI genes and characterization of vacA s, i and m regions in Helicobacter pylori isolated from Alaskans and their association with clinical pathologies.

  2020cites this paper
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  2019cites this paper
• Genetic diversity and virulence characteristics of Helicobacter pylori isolates in different human ethnic groups.

  2019cites this paper
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  2018cites this paper
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  2018cites this paper
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  2017cites this paper
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  2016cites this paper
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• What exists beyond cagA and vacA? Helicobacter pylori genes in gastric diseases.

  2015cites this paper
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  2015influential citation
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• Brazilian Journal of Microbiology (2012):

  2013cites this paper
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• Analysis of Helicobacter pylori genotypes in Afghani and Iranian isolates.

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  2008cites this paper
• Genome scale portrait of cAMP-receptor protein (CRP) regulons in mycobacteria points to their role in pathogenesis.

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  2007cites this paper
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  2007cites this paper
• genoBASE pylori: a genotype search tool and database of the human gastric pathogen Helicobacter pylori.

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• Ancestral European roots of Helicobacter pylori in India

  2007cites this paper
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  2006cites this paper
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