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Dynamics of Fucus serratus thallus photosynthesis and community primary production during emersion across seasons: canopy dampening and biochemical acclimation

A. Migné,Gwendoline Duong,D. Menu,D. Davoult,F. Gevaert

Published 2021 in Peer Community Journal

ABSTRACT

The brown alga Fucus serratus forms dense stands on the sheltered low intertidal rocky shores of the Northeast Atlantic coast. In the southern English Channel, these stands have proved to be highly productive, particularly during emersion periods. Here, we studied the dampening effect of the canopy cover, associated with physiological and biochemical acclimation processes, that allows this species to withstand emersion stress. The F. serratus community primary production and the photosynthetic performance of thalli were concurrently followed in situ, throughout the midday emersion period, in different seasons and under various weather conditions. In addi-tion, thallus samples were taken at various tidal stages to determine their content in biochemical compounds involved in photoprotective and antioxidant mechanisms. Under high light and temperature, 0.05 g of ground lyophilized thallus samples was homogenized with a glass pestle in an Eppendorf tube containing 1 mL of trichloroacetic acid (TCA). Homogenates centrifuged (10 min, 13 000 and aliquots of 0.2 mL of supernatant were added to a reaction mixture containing 0.2 mL of and 0.8 mL of iodide. After an The equivalent was obtained by using a TCA and a 0.01 H 2 O 2 . Ascorbate (Asc) using a protocol adapted Hodges 0.05 g of ground lyophilized thallus samples was homogenized with a glass pestle in an Eppendorf tube containing 1 mL of 2.5 perchloric acid. Homogenates were centrifuged (10 min, 13 000 rpm) and aliquots of 0.1 mL of supernatant were added to 5 μL of 100 dithiothreitol. After an incubation period of 1 h at room temperature, the was stopped by adding 5 μL of 5% n -ethylmale imide and 900 μL of a reaction mixture containing 2% TCA, 8.8% orthophosphoric 0.2% 2- 2’ -bipyridyl and 10 iron chloride and then incubated for 1 h at 40°C.

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