Two growth processes, cell proliferation and expansion, determine plant species-specific organ sizes. A large flower mutant in Arabidopsis thaliana, ohbana1 (ohb1), was isolated from a mutant library. In the ohb1 flowers, post-mitotic cell expansion and endoreduplication of nuclear DNA were promoted. The whole-genome resequencing and genetic analysis results showed that the loss of function in MEDIATOR16 (MED16), a mediator complex subunit, was responsible for the large flower phenotypes exhibited by ohb1. A phenotypic analysis of the mutant alleles in MED16 and the double mutants created by crossing ohb1 with representative large flower mutants revealed that MED16 and MED25 share part of the negative petal size regulatory pathways. Furthermore, the double mutant analyses suggested that there were genetically independent pathways leading to cell size restrictions in the floral organs which were not related to the MED complex. Several double mutants also formed larger and heavier seeds than the wild type and single mutant plants, which indicated that MED16 was involved in seed size regulation. This study has revealed part of the size-regulatory network in flowers and seeds through analysis of the ohb1 mutant, and that the size-regulation pathways are partially different between floral organs and seeds.
Double Mutant Analysis with the Large Flower Mutant, ohbana1, to Explore the Regulatory Network Controlling the Flower and Seed Sizes in Arabidopsis thaliana
Vuong Quoc Nhat,Y. Kazama,K. Ishii,S. Ohbu,H. Kunitake,T. Abe,T. Hirano
Published 2021 in Plants
ABSTRACT
PUBLICATION RECORD
- Publication year
2021
- Venue
Plants
- Publication date
2021-09-01
- Fields of study
Biology, Medicine, Environmental Science
- Identifiers
- External record
- Source metadata
Semantic Scholar, PubMed
CITATION MAP
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CLAIMS
CONCEPTS
- endoreduplication
A DNA replication process without cell division that increases nuclear DNA content.
Aliases: endoreplication
- floral organ cell size restriction
Mechanisms that limit cell size in floral organs such as petals and other flower tissues.
Aliases: flower cell-size restriction
- med16
A mediator complex subunit encoded by the affected gene implicated in organ-size regulation in this Arabidopsis study.
Aliases: Mediator16
- med25
Another mediator complex subunit examined for its role in regulating petal size alongside MED16.
Aliases: Mediator25
- ohbana1 (ohb1)
An Arabidopsis thaliana mutant line with enlarged flowers used as the focal genetic background in the study.
Aliases: ohb1, ohbana1
- petal size regulatory pathway
A regulatory pathway involved in controlling petal growth and final petal size.
Aliases: negative petal size pathway
- post-mitotic cell expansion
The growth phase after cell division in which differentiated cells increase in size.
Aliases: cell expansion after mitosis
- seed size regulation
The set of regulatory mechanisms that determine final seed size.
Aliases: seed-size control
REFERENCES
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