Surface histidine mutations for the metal affinity purification of a β-carbonic anhydrase.

No abstract is available for this paper.

• Publication year

  2014

• Venue

  Analytical Biochemistry

• Publication date

  2014-08-01

• Fields of study

  Medicine, Chemistry

• Identifiers
  DOI 10.1016/j.ab.2014.04.020PMID 24792153
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• NIH Image to ImageJ: 25 years of image analysis

  2012cited by this paper
• Structure and catalytic mechanism of the beta-carbonic anhydrases.

  2010cited by this paper
• Allosteric site variants of Haemophilus influenzae beta-carbonic anhydrase.

  2009cited by this paper
• Identification of a novel noncatalytic bicarbonate binding site in eubacterial beta-carbonic anhydrase.

  2006cited by this paper
• Structural analysis and classification of native proteins from E. coli commonly co-purified by immobilised metal affinity chromatography.

  2006cited by this paper
• Cloning, crystallization and preliminary characterization of a beta-carbonic anhydrase from Escherichia coli.

  2000cited by this paper
• Crystal structures of the copper and nickel complexes of RNase A: metal-induced interprotein interactions and identification of a novel copper binding motif.

  1997cited by this paper
• One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin.

  1995cited by this paper
• Activation and inhibition of bovine carbonic anhydrase III by dianions.

  1991cited by this paper
• The "megaprimer" method of site-directed mutagenesis.

  1990cited by this paper
• Calculation of protein extinction coefficients from amino acid sequence data.

  1989cited by this paper
• Activation parameters for the carbonic anhydrase II-catalyzed hydration of CO2.

  1986cited by this paper
• The carbon dioxide hydration activity of carbonic anhydrase. I. Stop-flow kinetic studies on the native human isoenzymes B and C.

  1971cited by this paper

• DigitalCommons@Providence DigitalCommons@Providence

  2021influential citation
• Designing of phenol-based β−carbonic anhydrase1 inhibitors through QSAR, molecular docking, and MD simulation approach

  2018cites this paper
• Surface Mutation Thr34His Facilitates Purification of Haemophilus influenza Carbonic Anhydrase via Metal Affinity Chromatography

  2017cites this paper
• Surface Mutation Thr 34 His Facilitates Purification of Haemophilus influenza Carbonic Anhydrase via Metal Affinity Chromatography

  2017influential citation
• E56H + E59H Mutation affects binding of the recombinant Haemphilus influenza carbonic anhydrase to a Ni-NTA Column

  2016influential citation
• A magnificent enzyme superfamily: carbonic anhydrases, their purification and characterization

  2016cites this paper
• Mutation of Surface Histidines in Haemophilus influenzae Carbonic Anhydrase to Enable Purification by Metal Affinity Chromatography

  2016cites this paper
• DigitalCommons@Providence DigitalCommons@Providence

  year unknowninfluential citation