The essential roles of OsFtsH2 in developing the chloroplast of rice

Background Filamentation temperature-sensitive H (FtsH) is an ATP-dependent zinc metalloprotease with ATPase activity, proteolysis activity and molecular chaperone-like activity. For now, a total of nine FtsH proteins have been encoded in rice, but their functions have not revealed in detail. In order to investigate the molecular mechanism of OsFtsH2 here, several osftsh2 knockout mutants were successfully generated by the CRISPR/Cas9 gene editing technology. Results All the mutants exhibited a phenotype of striking albino leaf and could not survive through the stage of three leaves. OsFtsH2 was located in the chloroplast and preferentially expressed in green tissues. In addition, osftsh2 mutants could not form normal chloroplasts and had lost photosynthetic autotrophic capacity. RNA sequencing analysis indicated that many biological processes such as photosynthesis-related pathways and plant hormone signal transduction were significantly affected in osftsh2 mutants. Conclusions Overall, the results suggested OsFtsH2 to be essential for chloroplast development in rice.

• Publication year

  2021

• Venue

  BMC Plant Biology

• Publication date

  2021-10-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1186/s12870-021-03222-zPMID 34598671PMCID 8485545
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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