Development of a multiplex assay for genus- and species-specific detection of Phytophthora based on differences in mitochondrial gene order.

No abstract is available for this paper.

• Publication year

  2014

• Venue

  Phytopathology

• Publication date

  2014-06-10

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1094/PHYTO-09-13-0263-RPMID 24915428
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Membrane-based oligonucleotide array developed from multiple markers for the detection of many Phytophthora species.

  2013cited by this paper
• Comparative Assessment of 5′ A/T-Rich Overhang Sequences with Optimal and Sub-optimal Primers to Increase PCR Yields and Sensitivity

  2013cited by this paper
• The genus Phytophthora anno 2012.

  2012cited by this paper
• Species Tree Estimation for the Late Blight Pathogen, Phytophthora infestans, and Close Relatives

  2012cited by this paper
• Identification and Detection of Phytophthora: Reviewing Our Progress, Identifying Our Needs.

  2012influential reference
• A universal microarray detection method for identification of multiple Phytophthora spp. using padlock probes.

  2012cited by this paper
• Development of an assay for rapid detection and quantification of Verticillium dahliae in soil.

  2012cited by this paper
• Characterisation and detection of Pythium and Phytophthora species associated with grapevines in South Africa

  2011influential reference
• The Plant Pathogen Phytophthora andina Emerged via Hybridization of an Unknown Phytophthora Species and the Irish Potato Famine Pathogen, P. infestans

  2011cited by this paper
• Phytophthora pini Leonian resurrected to distinct species status

  2011cited by this paper
• Phytophthora taxa associated with cultivated Agathosma, with emphasis on the P. citricola complex and P. capensis sp. nov.

  2010cited by this paper
• Phytophthora × pelgrandis, a new natural hybrid pathogenic to Pelargonium grandiflorum hort.

  2009cited by this paper
• Phytophthora multivora sp. nov., a new species recovered from declining Eucalyptus, Banksia, Agonis and other plant species in Western Australia

  2009cited by this paper
• Population Genetic Analysis Infers Migration Pathways of Phytophthora ramorum in US Nurseries

  2009influential reference
• Re-evaluation of Phytophthora citricola isolates from multiple woody hosts in Europe and North America reveals a new species, Phytophthora plurivora sp. nov.

  2009influential reference
• The avocado subgroup of Phytophthora citricola constitutes a distinct species, Phytophthora mengei sp. nov.

  2009cited by this paper
• Multiplex real-time polymerase chain reaction (PCR) for detection of Phytophthora ramorum, the causal agent of sudden oak death

  2009cited by this paper
• A multi-locus phylogeny for Phytophthora utilizing markers derived from complete genome sequences.

  2008cited by this paper
• IN REAL-TIME

  2008cited by this paper
• Molecular Detection of Phytophthora ramorum by Real-Time Polymerase Chain Reaction Using TaqMan, SYBR Green, and Molecular Beacons.

  2007cited by this paper
• Mitochondrial genome sequences and comparative genomics of Phytophthora ramorum and P. sojae

  2007cited by this paper
• Development and application of a PCR‐based ‘molecular tool box’ for the identification of Phytophthora species damaging forests and natural ecosystems

  2007cited by this paper
• Diagnostic Values and Utility of Immunological, Morphological, and Molecular Methods for In Planta Detection of Phytophthora ramorum.

  2007cited by this paper
• Primers with 5' flaps improve real-time PCR.

  2007cited by this paper
• Sudden oak death, Phytophthora ramorum

  2007cited by this paper
• Genetic characterization of the natural hybrid species Phytophthora alni as inferred from nuclear and mitochondrial DNA analyses.

  2006cited by this paper
• Real-Time Fluorescent Polymerase Chain Reaction Detection of Phytophthora ramorum and Phytophthora pseudosyringae Using Mitochondrial Gene Regions.

  2006cited by this paper
• Natural hybrids of resident and introduced Phytophthora species proliferating on multiple new hosts

  2006cited by this paper
• Detection and quantification of Phytophthora ramorum, P. kernoviae, P. citricola and P. quercina in symptomatic leaves by multiplex real-time PCR.

  2006cited by this paper
• Real-Time PCR

  2005cited by this paper
• Phytophthora kernoviae sp. nov., an invasive pathogen causing bleeding stem lesions on forest trees and foliar necrosis of ornamentals in the UK.

  2005cited by this paper
• Size variation of rDNA clusters in the yeasts Saccharomyces cerevisiea and Schizosaccharomyces pombe

  2004cited by this paper
• Molecular Detection of Phytophthora ramorum, the Causal Agent of Sudden Oak Death in California, and Two Additional Species Commonly Recovered from Diseased Plant Material.

  2004cited by this paper
• Non-oak native plants are main hosts for sudden oak death pathogen in California

  2003influential reference
• Sudden oak death: endangering California and Oregon forest ecosystems

  2003influential reference
• Phytophthora ramorum and sudden oak death in California: III. Preliminary studies in pathogen genetics.

  2002influential reference
• Real-Time Quantitative PCR

  2002cited by this paper
• Phylogenetic relationships among some Pythium species inferred from sequence analysis of the mitochondrially encoded cytochrome oxidase II gene

  2000cited by this paper
• Origin of a new Phytophthora pathogen through interspecific hybridization.

  1999cited by this paper
• BIOEDIT: A USER-FRIENDLY BIOLOGICAL SEQUENCE ALIGNMENT EDITOR AND ANALYSIS PROGRAM FOR WINDOWS 95/98/ NT

  1999cited by this paper
• Real time quantitative PCR.

  1996cited by this paper
• Yeasts have a four‐fold variation in ribosomal DNA copy number

  1993cited by this paper
• Variations in the number of ribosomal DNA units in morphological mutants and normal strains of Candida albicans and in normal strains of Saccharomyces cerevisiae

  1993cited by this paper
• Frequent changes in the number of reiterated ribosomal RNA genes throughout the life cycle of the basidiomycete Coprinus cinereus.

  1993cited by this paper
• Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics

  1990influential reference
• Premeiotic change of nucleolus organizer size in Neurospora.

  1989cited by this paper
• Comparison of two media selective for Phytophthora and Pythium species.

  1986cited by this paper

• Current understanding and future perspectives on pathogen biology and management of potato and tomato late blight (Phytophthora infestans) in Canada

  2025cites this paper
• Multivariate Geostatistical Modeling of Phytophthora rubi and Pratylenchus penetrans in Red Raspberry Fields

  2025cites this paper
• Synergistic 3D, multispectral, and thermal image analysis via supervised machine learning for improved detection of root rot symptoms in hydroponically-grown flat-leaf parsley

  2025cites this paper
• Enhanced TaqMan qPCR Assay for Phytophthora cinnamomi Detection and Quantification

  2025cites this paper
• Multiplex PCR specific for genus Phytophthora and P. nicotianae with an internal plant DNA control for effective quarantine of Phytophthora species in Japan

  2024cites this paper
• TaqMan qPCR Detection and Quantification of Phytophthora cinnamomi in Soil and Plant Tissues for Walnut Disease Management

  2024cites this paper
• A systematic approach for identifying unique genomic sequences for Fusarium oxysporum f. sp. lactucae race 1 and development of molecular diagnostic tools.

  2024cites this paper
• A rapid quarantine approach for the pathogenic and invasive Phytophthora species associated with imported fruits in China.

  2024cites this paper
• Ultra-sensitive detection of Phytophthora pluvialis by real-time PCR targeting a mitochondrial gene

  2024cites this paper
• Use of Phosphite Preparations to Protect Ash Seedlings Fraxinus excelsior L. against Phytophthora spp. and Hymenoscyphus fraxineus Pathogens

  2024cites this paper
• A qPCR assay for the detection of Phytophthora abietivora, an emerging pathogen on fir species cultivated as Christmas trees

  2024cites this paper
• Worldwide forest surveys reveal forty-three new species in Phytophthora major Clade 2 with fundamental implications for the evolution and biogeography of the genus and global plant biosecurity.

  2024cites this paper
• Diagnosis of Soybean Diseases Caused by Fungal and Oomycete Pathogens: Existing Methods and New Developments

  2023cites this paper
• Pre-Service Teachers’ Experience of Learning about Sustainability in Technology Education in South Africa

  2023cites this paper
• Climate change, a booster of disease outbreaks by the plant pathogen Phytophthora in oak forests

  2023cites this paper
• First report of

  2023cites this paper
• Simultaneous detection of three crown rot pathogens in field-grown strawberry plants using a multiplex PCR assay

  2022cites this paper
• Optimizing molecular detection for the hop downy mildew pathogen Pseudoperonospora humuli in plant tissue.

  2022cites this paper
• Characterization of a Novel Double-Stranded RNA Virus from Phytophthora pluvialis in New Zealand

  2022cites this paper
• Early detection of the spinach downy mildew pathogen in leaves by recombinase polymerase amplification.

  2022cites this paper
• A step towards validation of high-throughput sequencing for the identification of plant pathogenic oomycetes.

  2022cites this paper
• Mitochondrial loci enable specific qPCR detection of the pathogen causing contemporary impatiens downy mildew epidemics.

  2021cites this paper
• Application of Real-Time PCR for the Detection and Quantification of Oomycetes in Ornamental Nursery Stock

  2021cites this paper
• A multiplex TaqMan qPCR assay for detection and quantification of clade 1 and clade 2 isolates of Pseudoperonospora cubensis and Pseudoperonospora humuli.

  2021cites this paper
• Fantastic Downy Mildew Pathogens and How to Find Them: Advances in Detection and Diagnostics

  2021influential citation
• Susceptibility of Hop Cultivars and Rootstock to Downy Mildew Caused by Pseudoperonospora humuli

  2021cites this paper
• Diversity of Phytophthora Communities across Different Types of Mediterranean Vegetation in a Nature Reserve Area

  2020cites this paper
• Disease-Induced Microbial Shifts in Citrus Indicate Microbiome-Derived Responses to Huanglongbing Across the Disease Severity Spectrum

  2020cites this paper
• Phytophthora Diversity in Lake Mathews, the Irrigation Water Source for a Southern California Nursery

  2020cites this paper
• Growth, sporulation, and pathogenicity of the raspberry pathogen Phytophthora rubi under different temperature and moisture regimes.

  2020cites this paper
• Development and evaluation of a recombinase polymerase amplification assay for rapid detection of strawberry red stele pathogen

  2020cites this paper
• RPA-PCR couple: an approach to expedite plant diagnostics and overcome PCR inhibitors

  2020influential citation
• Monitoring of Peronospora destructor Primary and Secondary Inoculum by Real-Time qPCR.

  2020cites this paper
• Validation of a Preformulated, Field Deployable, Recombinase Polymerase Amplification Assay for Phytophthora Species

  2020cites this paper
• Promising Perspectives for Detection, Identification, and Quantification of Plant Pathogenic Fungi and Oomycetes through Targeting Mitochondrial DNA

  2020cites this paper
• Metabarcoding for Phytophthora —benefits and limitations

  2020cites this paper
• Phytophthora Species Are Common on Nursery Stock Grown for Restoration and Revegetation Purposes in California.

  2019cites this paper
• Detection and Quantification of Pythium tracheiphilum in Soil by Multiplex Real-Time qPCR.

  2019cites this paper
• Detection of Fusarium oxysporum f. sp. fragariae from Infected Strawberry Plants.

  2019cites this paper
• Recombinase Polymerase Amplification Assay for fast, sensitive and on-site detection of Phytophthora cactorum without DNA extraction

  2019cites this paper
• New Oomycota Fungicides With Activity Against Phytophthora cinnamomi and Their Potential Use for Managing Avocado Root Rot in California.

  2019cites this paper
• High‐resolution biomonitoring of plant pathogens and plant species using metabarcoding of pollen pellet contents collected from a honey bee hive

  2019cites this paper
• Novel approaches and methods for quantifying Phytophthora cinnamomi in avocado tree roots.

  2019cites this paper
• Phenotypic Characterization of Genetically Distinct Phytophthora cinnamomi Isolates from Avocado.

  2019cites this paper
• In Situ Processing and Efficient Environmental Detection (iSPEED) of tree pests and pathogens using point-of-use real-time PCR

  2019cites this paper
• High-Throughput Sequencing to Investigate Phytopathogenic Fungal Propagules Caught in Baited Insect Traps

  2019cites this paper
• A Novel Phagomyxid Parasite Produces Sporangia in Root Hair Galls of Eelgrass (Zostera marina).

  2019cites this paper
• Screening for Exotic Forest Pathogens to Increase Survey Capacity Using Metagenomics.

  2018cites this paper
• Differentiation of Phytophthora species associated with plantation crops using PCR and high-resolution melting curve analysis

  2018cites this paper
• Metaxa2 Database Builder: enabling taxonomic identification from metagenomic or metabarcoding data using any genetic marker

  2018cites this paper
• A multiplex PCR assay for three pathogenic Phytophthora species related to kiwifruit diseases in China

  2018cites this paper
• Next-generation sequencing to investigate existing and new insect associations with phytopathogenic fungal propagules

  2018cites this paper
• Advances in Diagnostics of Downy Mildews: Lessons Learned from Other Oomycetes and Future Challenges.

  2018cites this paper
• An Overview of Canadian Research Activities on Diseases Caused by Phytophthora ramorum: Results, Progress, and Challenges.

  2018cites this paper
• Differentiation of Phytophthora species associated with plantation crops using PCR and high-resolution melting curve analysis

  2018cites this paper
• Development of seven novel specific SCAR markers for rapid identification of Phytophthora sojae: the cause of root- and stem-rot disease of soybean

  2018cites this paper
• Analysis of chitinase activity

  2017cites this paper
• Late-summer Disease Symptoms in Western Washington Red Raspberry Fields Associated with Co-Occurrence of Phytophthora rubi, Verticillium dahliae, and Pratylenchus penetrans, but not Raspberry bushy dwarf virus.

  2017cites this paper
• Development and Application of qPCR and RPA Genus- and Species-Specific Detection of Phytophthora sojae and P. sansomeana Root Rot Pathogens of Soybean.

  2017cites this paper
• Development of Real-Time Isothermal Amplification Assays for On-Site Detection of Phytophthora infestans in Potato Leaves.

  2017cites this paper
• Systematic Development of Phytophthora Species-Specific Mitochondrial Diagnostic Markers for Economically Important Members of the Genus.

  2017cites this paper
• DNA Barcoding for Diagnosis and Monitoring of Fungal Plant Pathogens

  2017cites this paper
• Molecular approaches for biosurveillance of the cucurbit downy mildew pathogen, Pseudoperonospora cubensis

  2017cites this paper
• Temporal Occurrence and Niche Preferences of Phytophthora spp. Causing Brown Rot of Citrus in the Central Valley of California.

  2017cites this paper
• Phytophthora parasitica and lupin (Lupinus angustifolius) interactions: changes in gene expression during infection and after phosphite treatment

  2017cites this paper
• Separation and concentration of Phytophthora ramorum sporangia by inertial focusing in curving microfluidic flows

  2016cites this paper
• Separation and concentration of Phytophthora ramorum sporangia by inertial focusing in curving microfluidic flows

  2016cites this paper
• First Report of Phytophthora cinnamomi Causing Root Rot of Southern Highbush Blueberry In California

  2016cites this paper
• Multiplex Detection and Identification of Phytophthora spp. Using Target Specific Primer Extension and Luminex xTAG Technology

  2016cites this paper
• Identification of the Dominant Genotypes of Phytophthora infestans in Canada Using Real-Time PCR with ASO-PCR Assays.

  2016cites this paper
• Detection and Quantification of Bremia lactucae by Spore Trapping and Quantitative PCR.

  2016cites this paper
• Metabarcoding Analysis of Phytophthora Diversity Using Genus-Specific Primers and 454 Pyrosequencing.

  2016cites this paper
• A Tale of Tails: Dissecting the Enhancing Effect of Tailed Primers in Real-Time PCR

  2016cites this paper
• Specific and Sensitive Detection of Phytophthora nicotianae by Nested PCR and Loop-mediated Isothermal Amplification Assays

  2015cites this paper
• Development of an FgMito assay: A highly sensitive mitochondrial based qPCR assay for quantification of Fusarium graminearum sensu stricto.

  2015cites this paper
• Development of a multiplex PCR assay for rapid and simultaneous detection of rubber tree pathogens Phytophthora spp . and P . palmivora

  2015cites this paper
• Molecular Detection of 10 of the Most Unwanted Alien Forest Pathogens in Canada Using Real-Time PCR

  2015cites this paper
• Development of Rapid Isothermal Amplification Assays for Detection of Phytophthora spp. in Plant Tissue.

  2015cites this paper
• Integrated air stream micromixer for performing bioanalytical assays on a plastic chip.

  2014cites this paper
• Membrane-based oligonucleotide array developed from multiple markers for the detection of many Phytophthora species.

  2013cites this paper