Effect of UVA Fluence Rate on Indicators of Oxidative Stress in Human Dermal Fibroblasts

During the course of a day human skin is exposed to solar UV radiation that fluctuates in fluence rate within the UVA (290-315 nm) and UVB (315-400 nm) spectrum. Variables affecting the fluence rate reaching skin cells include differences in UVA and UVB penetrating ability, presence or absence of sunscreens, atmospheric conditions, and season and geographical location where the exposure occurs. Our study determined the effect of UVA fluence rate in solar-simulated (SSR) and tanning-bed radiation (TBR) on four indicators of oxidative stress---protein oxidation, glutathione, heme oxygenase-1, and reactive oxygen species--in human dermal fibroblasts after receiving equivalent UVA and UVB doses. Our results show that the higher UVA fluence rate in TBR increases the level of all four indicators of oxidative stress. In sequential exposures when cells are exposed first to SSR, the lower UVA fluence rate in SSR induces a protective response that protects against oxidative stress following a second exposure to a higher UVA fluence rate. Our studies underscore the important role of UVA fluence rate in determining how human skin cells respond to a given dose of radiation containing both UVA and UVB radiation.

• Publication year

  2008

• Venue

  International Journal on Biological Sciences

• Publication date

  2008-02-19

• Fields of study

  Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.7150/IJBS.4.63PMID 18311331PMCID 2253953
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The dose rate of UVA treatment influences the cellular response of HaCaT keratinocytes.

  2008cited by this paper
• Radiation sources providing increased UVA/UVB ratios attenuate the apoptotic effects of the UVB waveband UVA-dose-dependently in hairless mouse skin.

  2007cited by this paper
• How different wavelengths of the ultraviolet spectrum contribute to skin carcinogenesis: the role of cellular damage responses.

  2007cited by this paper
• Relevance of Sunscreen Application Method, Visible Light and Sunlight Intensity to Free-radical Protection: A Study of ex vivo Human Skin

  2006cited by this paper
• Radiation Sources Providing Increased UVA/UVB Ratios Induce Photoprotection Dependent on the UVA Dose in Hairless Mice

  2006cited by this paper
• Original Contribution Overexpression of MsrA protects WI-38 SV40 human fibroblasts against H 2 O 2 -mediated oxidative stress

  2005cited by this paper
• The repair enzyme peptide methionine-S-sulfoxide reductase is expressed in human epidermis and upregulated by UVA radiation.

  2005cited by this paper
• Ultraviolet A irradiation induces NF-E2-related factor 2 activation in dermal fibroblasts: protective role in UVA-induced apoptosis.

  2005cited by this paper
• UVA-induced oxidative damage and cytotoxicity depend on the mode of exposure.

  2005cited by this paper
• Overexpression of MsrA protects WI-38 SV40 human fibroblasts against H2O2-mediated oxidative stress.

  2005cited by this paper
• Solar Ultraviolet A Radiation: An Oxidizing Skin Carcinogen that Activates Heme Oxygenase-1

  2004cited by this paper
• Singlet oxygen-mediated damage to proteins and its consequences.

  2003cited by this paper
• Protein carbonyl groups as biomarkers of oxidative stress.

  2003cited by this paper
• What is light?

  2002cited by this paper
• Ultraviolet A and melanoma: a review.

  2001cited by this paper
• Chemiluminescent detection and imaging of reactive oxygen species in live mouse skin exposed to UVA.

  2000cited by this paper
• Macromolecular carbonyls in human stratum corneum: a biomarker for environmental oxidant exposure?

  1998cited by this paper
• Oxidative stress and in vivo chemiluminescence in mouse skin exposed to UVA radiation.

  1997cited by this paper
• TWO GENES CONTRIBUTE TO DIFFERENT EXTENTS TO THE HEME OXYGENASE ENZYME ACTIVITY MEASURED IN CULTURED HUMAN SKIN FIBROBLASTS AND KERATINOCYTES: IMPLICATIONS FOR PROTECTION AGAINST OXIDANT STRESS

  1995cited by this paper
• Ultraviolet radiation and free radical damage to skin.

  1995cited by this paper
• UVA radiation-induced oxidative damage to lipids and proteins in vitro and in human skin fibroblasts is dependent on iron and singlet oxygen.

  1995cited by this paper
• Heme oxygenase 1 mediates an adaptive response to oxidative stress in human skin fibroblasts.

  1994cited by this paper
• Ultraviolet A-induced lipid peroxidation and antioxidant defense systems in cultured human skin fibroblasts.

  1993cited by this paper
• Oxidative stress resulting from ultraviolet A irradiation of human skin fibroblasts leads to a heme oxygenase-dependent increase in ferritin.

  1993cited by this paper
• Endogenous glutathione levels modulate the frequency of both spontaneous and long wavelength ultraviolet induced mutations in human cells.

  1992cited by this paper
• Heme oxygenase is the major 32-kDa stress protein induced in human skin fibroblasts by UVA radiation, hydrogen peroxide, and sodium arsenite.

  1989cited by this paper
• Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine.

  1980cited by this paper
• What Is Light

  1968cited by this paper
• Photochemische Untersuchungen

  year unknowncited by this paper

• Solidified Salt Melts of the NaCl–KCl–CeF3–EuF3 System as Promising Luminescent Materials

  2024cites this paper
• Mitochondria-Targeted Hydrogen Sulfide Delivery Molecules Protect Against UVA-Induced Photoaging in Human Dermal Fibroblasts, and in Mouse Skin In Vivo

  2021cites this paper
• Wavelengths and temporal effects on the response of mammalian cells to UV radiation: Limitations of action spectra illustrated by genotoxicity.

  2021cites this paper
• Caffeic acid derivatives isolated from Galinsoga parviflora herb protected human dermal fibroblasts from UVA-radiation.

  2019cites this paper
• Monitoring the Genotoxic Potential of Sunlight and DNA Photoprotection of Sunscreen

  2019cites this paper
• Skin Aging & Cancer: Ambient UV-R Exposure

  2019cites this paper
• UVA1 impairs the repair of UVB‐induced DNA damage in normal human melanocytes

  2018cites this paper
• 3-Amino-1,2,4-triazole Limits the Oxidative Damage in UVA-Irradiated Dysplastic Keratinocytes

  2017cites this paper
• Genetic resources and breeding methodologies for improving drought tolerance in wheat

  2017cites this paper
• Sunlight damage to cellular DNA: Focus on oxidatively generated lesions.

  2017cites this paper
• Ultraviolet A Radiation and ROS: Observations from Studies with Bacteria

  2016cites this paper
• Skin Treatment with Pulsed Monochromatic UVA1 355 Device and Computerized Morphometric Analysis of Histochemically Identified Langerhans Cells

  2016cites this paper
• Identification of influential events concerning the Antarctic ozone hole over southern Brazil and the biological effects induced by UVB and UVA radiation in an endemic treefrog species.

  2015cites this paper
• Identification of influential events concerning the Antarctic ozone hole over southern Brazil and the biological effects induced by UVB and UVA radiation in an endemic treefrog species.

  2015cites this paper
• Topical Retinoids in Skin Ageing: A Focused Update with Reference to Sun-Induced Epidermal Vitamin A Deficiency

  2014cites this paper
• Determining the action spectrum of UVR-induced mitochondrial DNA damage, and related UVR-induced cellular effects in human skin

  2013cites this paper
• Hydrogen water intake via tube-feeding for patients with pressure ulcer and its reconstructive effects on normal human skin cells in vitro

  2013cites this paper
• DNA damage as a biological sensor for environmental sunlight

  2013cites this paper
• Extrafollicular Dermal Melanocyte Stem Cells and Melanoma

  2012cites this paper
• Low-Dose UVA Radiation-Induced Adaptive Response in Cultured Human Dermal Fibroblasts

  2012cites this paper
• Ellagic acid protects human keratinocyte (HaCaT) cells against UVA-induced oxidative stress and apoptosis through the upregulation of the HO-1 and Nrf-2 antioxidant genes.

  2012cites this paper
• UVA Irradiation of Dysplastic Keratinocytes: Oxidative Damage versus Antioxidant Defense

  2012cites this paper
• Ultraviolet radiation and echinoderms: past, present and future perspectives.

  2011cites this paper
• Mechanisms of cell damage in enteric bacteria during solar disinfection (SODIS)

  2010cites this paper
• Pathogenic role of angiotensin II and oxidised LDL in obstructive sleep apnoea

  2009cites this paper
• Cellular and sub-cellular responses to UVA in relation to carcinogenesis

  2009cites this paper
• Solar disinfection (SODIS) and subsequent dark storage of Salmonella typhimurium and Shigella flexneri monitored by flow cytometry.

  2009cites this paper