Exploration of New Mutations for Resistance of Houseflies to Organophosphate Insecticides in Housefly Populations in Guizhou Province, China

Background： The campaign to establish the national sanitary city has been launched across major places in Guizhou Province, which leads to the extensive use of insecticide to eradicate the disease-carrier Musca domestica found everywhere while keeping the cleanliness of environment.Methods: In order to perceive the resistance of houseflies to the commonly used organophosphate insecticides in 7 housefly populations belonging to Guizhou province (China), the susceptibility bioassays, detection of resistance-associated mutations, and the carboxylesterase activity assay were conducted.Results: The bioassays exhibited 142.16~303.54-fold to dichlorvos (DDVP) and 122.13~363.98-fold to temephos. The molecular analysis unveiled mutant ACE gene at loci of 260, 342 and 407 in all populations, which led to high frequencies at 27.4~73.8% of 260L, 59.1~76.7% of 342A, 23.7~40.9% of 342V, and 83.4~100.0% of 407Y, with inclusion of 8 genotypes and 10 mutant combinations. Further analysis of mutations showed a linkage disequilibrium of L/V+A+Y at locus 260 & 342, indicating a significant association with the DDVP resistance. The Hardy-Weinberg equilibrium test demonstrated that the observed ACE heterozygosity mostly exceeded 0.5 and deviated from the equilibrium. In the fixation index, an insignificant differentiation was noted among the 7 housefly populations. Conclusions: However, further research should concentrate on the use of insecticides to avoid the abuse of insecticides, and to regularly monitor the resistance of houseflies using novel methods.

• Publication year

  2020

• Venue

  Unknown venue

• Publication date

  2020-07-15

• Fields of study

  Not labeled

• Identifiers
  DOI 10.21203/rs.3.rs-41236/v1
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

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• No concepts are published for this paper.

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