Molecular detection of 2c variant of Carnivore protoparvovirus1 from Mizoram, India

Canine parvovirus2 (CPV2) is associated with acute fatal hemorrhagic diarrhea in pups. The present study was conducted to detect the variant of Carnivore protoparvovirus 1 from a case of severe hemorrhagic gastroenteritis in a pup by polymerase chain reaction (PCR) combining with partial VP2 gene sequencing. PCR was performed, targeting a partial segment of the VP2 protein gene, which gives an amplicon of 536 bp. PCR amplicon was cloned and sequenced. Phylogenetic analysis was performed by comparing sequences with all other variants of CPV2 viruses, revealed that the virus belonged to the CPV2c variant (MN218607). The partial VP2 gene sequence revealed a 99.4 to 99.6% sequence similarity with other reported CPV2c variant. Typical substitution of Glutamine (E) instead of Asparagine (N) in the amino acid position 426 could be observed, further confirming the CPV2c variant. Till now, there is no published report available that show the presence of CPV 2c variant in the dog population of Mizoram.

• Publication year

  2020

• Venue

  Journal of Entomology and Zoology Studies

• Publication date

  2020-11-01

• Fields of study

  Not labeled

• Identifiers
  DOI 10.22271/j.ento.2020.v8.i6n.7973
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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