A Weak TATA Box Is a Prerequisite for Glucocorticoid-dependent Repression of the Osteocalcin Gene*

The TATA box element is not only important for establishing basal levels of transcription, but it can also be used to modulate cell type or stage specific gene activity. In the case of the human osteocalcin gene, which is transcriptionally repressed by glucocorticoids, a specific binding element for the glucocorticoid receptor (GR) overlaps a noncanonical TATA box. In the present study, the relevance and function of the TATA element in glucocorticoid-mediated repression of the human osteocalcin gene was characterized. Mutating this noncanonical TATA box into a consensus TATA box within the context of the osteocalcin promoter greatly decreased hormone-dependent transcriptional repression by GR. TATA-binding protein (TBP) bound this mutated element much more strongly suggesting a physiologically relevant role for the weak osteocalcin TATA element in the regulation of this bone specific gene. The optimization of the putative transcription factor IIB recognition site did not affect the level of GR-mediated repression. Our results support a model wherein competitive DNA binding of GR and TBP for their overlapping sites explains conditional repression of the osteocalcin gene by glucocorticoids.

• Publication year

  1997

• Venue

  Journal of Biological Chemistry

• Publication date

  1997-12-05

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/jbc.272.49.30709PMID 9388207
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Glucocorticoid-Dependent Transcriptional Repression of the Osteocalcin Gene by Competitive Binding at the TATA Box

  1997cited by this paper
• Identification of a negative glucocorticoid response element in the rat type 1 vasoactive intestinal polypeptide receptor gene.

  1996cited by this paper
• High-resolution mapping of nucleoprotein complexes by site-specific protein-DNA photocrosslinking: organization of the human TBP-TFIIA-TFIIB-DNA quaternary complex.

  1996cited by this paper
• Contacts in context: promoter specificity and macromolecular interactions in transcription.

  1996cited by this paper
• Biochemistry and structural biology of transcription factor IID (TFIID).

  1996cited by this paper
• Crystal structure of a TFIIB–TBP–TATA-element ternary complex

  1995cited by this paper
• Model for binding of transcription factor TFIIB to the TBP-DNA complex

  1995cited by this paper
• DNA bending is an important component of site-specific recognition by the TATA binding protein.

  1995cited by this paper
• An inverted TATA box directs downstream transcription of the bone sialoprotein gene.

  1995cited by this paper
• Two distinct factors bind to the rabbit uteroglobin TATA-box region and are required for efficient transcription

  1994cited by this paper
• Human androgen receptor expressed in HeLa cells activates transcription in vitro.

  1994cited by this paper
• The c-fos serum response element (SRE) confers negative response to glucocorticoids.

  1994cited by this paper
• Crystal structure of a yeast TBP/TATA-box complex

  1993cited by this paper
• Inhibition of Gene Expression by Steroid Hormone Receptors Via a Negative Glucocorticoid Response Element: Evidence for the Involvement of DNA-Binding and Agonistic Effects of the Antiglucocorticoid/Antiprogestin RU486

  1993cited by this paper
• Analysis of Baculovirus‐Expressed Androgen Receptors

  1993cited by this paper
• Co-crystal structure of TBP recognizing the minor groove of a TATA element

  1993cited by this paper
• Yeast and human TFIID with altered DNA-binding specificity for TATA elements.

  1992cited by this paper
• Overexpression of receptors of the steroid/thyroid family.

  1992cited by this paper
• Transcription factor TFIID induces DNA bending upon binding to the TATA element.

  1992cited by this paper
• Kinetic analysis of yeast TFIID-TATA box complex formation suggests a multi-step pathway.

  1992cited by this paper
• TFIID can be rate limiting in vivo for TATA-containing, but not TATA-lacking, RNA polymerase II promoters.

  1992cited by this paper
• The glucocorticoid receptor binds to a sequence overlapping the TATA box of the human osteocalcin promoter: a potential mechanism for negative regulation

  1991cited by this paper
• High level expression of functional full length and truncated glucocorticoid receptor in Chinese hamster ovary cells. Demonstration of ligand-induced down-regulation of expressed receptor mRNA and protein.

  1991cited by this paper
• Yeast and human TATA-binding proteins have nearly identical DNA sequence requirements for transcription in vitro

  1990cited by this paper
• On the mechanism for efficient repression of the interleukin-6 promoter by glucocorticoids: enhancer, TATA box, and RNA start site (Inr motif) occlusion

  1990cited by this paper
• A wide variety of DNA sequences can functionally replace a yeast TATA element for transcriptional activation.

  1990cited by this paper
• Negative regulation of the thyroid-stimulating hormone alpha gene by thyroid hormone: receptor interaction adjacent to the TATA box.

  1989cited by this paper
• 1,25-dihydroxyvitamin D-responsive element and glucocorticoid repression in the osteocalcin gene.

  1989cited by this paper
• Fine structure genetic analysis of a beta-globin promoter.

  1986cited by this paper
• Rapid and efficient site-specific mutagenesis without phenotypic selection.

  1985cited by this paper
• Organization and expression of eucaryotic split genes coding for proteins.

  1981cited by this paper

• Alpha-tubulin promoter from Chlorella vulgaris allows genetic transformation of green coccoid microalga

  2020cites this paper
• Glucocorticoids as an adjunct to oncologic treatment in solid malignancies - Not an innocent bystander.

  2018cites this paper
• Age-related and seasonal variations in plasma uncarboxylated osteocalcin in male Murrah buffaloes

  2017cites this paper
• Modelling the glucocorticoid receptor dimerisation cycle

  2017cites this paper
• Effect of kidney tonifying prescriptions- Liu wei di huang Wan and Ba wei di huang Wan on insulin resistance

  2014cites this paper
• Class I Lysine Deacetylases Facilitate Glucocorticoid Receptor-Mediated Transcriptional Activation

  2013cites this paper
• Transcriptional Regulation of The Porcine GnRH Receptor Gene by Glucocorticoids

  2013cites this paper
• Juvenile hormone action through a defined enhancer motif to modulate ecdysteroid-activation of natural core promoters.

  2012cites this paper
• Modulation of GR transcriptional signalling by HIV-1 Vpr: insights into regulation by progestins

  2012cites this paper
• Glucocorticoid receptor and histone deacetylase-2 mediate dexamethasone-induced repression of MUC5AC gene expression.

  2012cites this paper
• The Double Helix in Motion: New Insights into Sequence-specific, Functional DNA Dynamics Using NMR Spectroscopy

  2011cites this paper
• Molecular modulation of osteocalcin and its relevance in diabetes (Review).

  2011cites this paper
• Discovery of selective glucocorticoid receptor modulator MK-5932.

  2011cites this paper
• Transient Hoogsteen Base Pairs in Canonical Duplex DNA

  2011cites this paper
• ROS & energy production pathways in the determination of resistance/sensitivity to glucocorticoids-induced apoptosis in acute lymphoblastic leukaemia

  2011cites this paper
• Xenobiotic-dependent regulation of human karyopherin A2 (KPNA2) gene.

  2011cites this paper
• Pharmacological strategies for improving the efficacy and therapeutic ratio of glucocorticoids in inflammatory lung diseases.

  2010cites this paper
• Classic glucocorticoids versus non-steroidal glucocorticoid receptor modulators: survival of the fittest regulator of the immune system?

  2010cites this paper
• Sexually Dimorphic Actions of Glucocorticoids Provide a Link to Inflammatory Diseases with Gender Differences in Prevalence

  2010cites this paper
• Glucocorticoid-Induced Osteoporosis: A Review

  2010influential citation
• Mechanisms Generating Diversity in Glucocorticoid Receptor Signaling

  2009cites this paper
• Selective amplification of glucocorticoid anti-inflammatory activity through synergistic multi-target action of a combination drug

  2009cites this paper
• Mécanismes de régulation transcriptionnelle du gène de l'alpha-foetoprotéine

  2009cites this paper
• Crosstalk in inflammation: the interplay of glucocorticoid receptor-based mechanisms and kinases and phosphatases.

  2009cites this paper
• PRE- AND POSTTRANSLATIONAL MECHANISMS OF REGULATION OF THE TYPE 3 IODOTHYRONINE DEIODINASE

  2009cites this paper
• Conservation analysis predicts in vivo occupancy of glucocorticoid receptor-binding sequences at glucocorticoid-induced genes

  2008cites this paper
• Epigenetic Regulation of HYAL-1 Hyaluronidase Expression

  2008cites this paper
• Gene expression in normotopic and heterotopic human bone: increased level of SP7 mRNA in pathological tissue

  2008cites this paper
• General Transcription Factor IIA-γ Increases Osteoblast-specific Osteocalcin Gene Expression via Activating Transcription Factor 4 and Runt-related Transcription Factor 2*

  2008cites this paper
• An antisense transcript spanning the CGG repeat region of FMR1 is upregulated in premutation carriers but silenced in full mutation individuals.

  2007cites this paper
• The regulation of preadipocyte differentiation by glucocorticoids

  2007cites this paper
• A reappraisal of the clinical efficacy of nebulized flunisolide in pediatric asthma: the Italian experience.

  2007cites this paper
• CHAPTER 1 – Glucocorticoids and Immunity: Mechanisms of Regulation

  2007cites this paper
• Glucocorticoid Signaling in Health and Disease

  2007cites this paper
• Noncanonical TATA Sequence in the UL44 Late Promoter of Human Cytomegalovirus Is Required for the Accumulation of Late Viral Transcripts

  2007influential citation
• Separating Transrepression and Transactivation: A Distressing Divorce for the Glucocorticoid Receptor?

  2007cites this paper
• Differential Mechanisms of Transcriptional Regulation of the Mouse Osteocalcin Gene by Jun Family Members

  2007cites this paper
• Variations in Intracellular Levels of TATA Binding Protein Can Affect Specific Genes by Different Mechanisms

  2007cites this paper
• Effects of dissociated glucocorticoids on OPG and RANKL in osteoblastic cells.

  2006cites this paper
• Dexamethasone-mediated repression of MUC5AC gene expression in human lung epithelial cells.

  2006cites this paper
• Effects of dissociated glucocorticoids on OPG and RANKL in osteoblastic cells

  2006cites this paper
• Non-optimal TATA Elements Exhibit Diverse Mechanistic Consequences*

  2006cites this paper
• Cross-talk between nuclear receptors and nuclear factor κB

  2006cites this paper
• The human glucocorticoid receptor: one gene, multiple proteins and diverse responses.

  2005cites this paper
• [Molecular mechanisms of glucocorticoids].

  2005cites this paper
• The role of glucocorticoid action in the pathophysiology of the Metabolic Syndrome

  2005cites this paper
• The search for safer glucocorticoid receptor ligands.

  2005cites this paper
• Glucocorticoids inhibit osteocalcin transcription in osteoblasts by suppressing Egr2/Krox20-binding enhancer.

  2005cites this paper
• Mecanismos moleculares de los glucocorticoides

  2005cites this paper
• Modulation of Folate Receptor-alpha by Glucocorticoid Receptor and Progesterone Receptor

  2005cites this paper
• An investigation of the role of phosphorylation at Ser211 of the glucocorticoid receptor in ligand-specific transcriptional regulation

  2005cites this paper
• EFFETS DES GLUCOCORTICOIDES SUR LA MISE EN PLACE DES CENTRES D'OSSIFICATION CHEZ L'EMBRYON DE RAT. Implication de certains g

  2004cites this paper
• Mechanisms of glucocorticoid receptor signaling during inflammation.

  2004cites this paper
• [Glucocorticoids and their receptor: mechanisms of action and clinical implications].

  2004cites this paper
• Les glucocorticoïdes et leur récepteur : mécanismes d'action et conséquences cliniques

  2004cites this paper
• Negative glucocorticoid receptor response elements and their role in glucocorticoid action.

  2004cites this paper
• Mechanisms of glucocorticoid signalling.

  2004cites this paper
• Limited specificity of promoter constructs for gene therapy in osteosarcoma.

  2004cites this paper
• Changing paradigms in dermatology: nuclear hormone receptors.

  2003cites this paper
• Inhaled glucocorticoids and adrenal function: an update.

  2003cites this paper
• Update on glucocorticoid action and resistance.

  2003cites this paper
• Glucocorticoid suppression of nuclear factor-kappa B: a role for histone modifications.

  2003cites this paper
• Regulation of NF-кB by Glucocorticoids

  2003cites this paper
• The interplay between the glucocorticoid receptor and nuclear factor-kappaB or activator protein-1: molecular mechanisms for gene repression.

  2003cites this paper
• Subunit selectivity of the crosstalk between GR and AP-1

  2003cites this paper
• Subunit Selectivity of the Crosstalk between GR and AP-1

  2003cites this paper
• Binding of the Thyroid Hormone Receptor to a Negative Element in the Basal Growth Hormone Promoter Is Associated with Histone Acetylation*

  2003cites this paper
• Regulation of Human Osteocalcin Promoter in Hormone-independent Human Prostate Cancer Cells*

  2002cites this paper
• Regulation of Osteocalcin Gene Expression by a Novel Ku Antigen Transcription Factor Complex*

  2002cites this paper
• The Glucocorticoid Receptor Represses the Positive Autoregulation of the Trout Estrogen Receptor Gene by Preventing the Enhancer Effect of a C/EBPβ-Like Protein.

  2002cites this paper
• Regulation of Glucocorticoid Receptor Function During the Cell Cycle

  2002cites this paper
• Steroid Hormones and Cell Cycle Regulation

  2002cites this paper
• Mechanisms involved in the side effects of glucocorticoids.

  2002cites this paper
• Review of the molecular and cellular mechanisms of action of glucocorticoids for use in asthma.

  2002cites this paper
• Molecular Mechanisms of Glucocorticoid Action

  2001cites this paper
• Cross‐talk between pro‐inflammatory transcription factors and glucocorticoids

  2001cites this paper
• Signal transduction by tumor necrosis factor and gene regulation of the inflammatory cytokine interleukin-6.

  2000cites this paper
• Molecular mechanisms of glucocorticosteroid actions.

  2000cites this paper
• In vitro and in vivo evidence for orphan nuclear receptor RORalpha function in bone metabolism.

  2000cites this paper
• Molecular mechanisms of glucocorticoid action: what is important?

  2000cites this paper
• Glucocorticoid-induced osteoporosis

  2000cites this paper
• Regulation of expression of the rat SOCS-3 gene in hepatocytes by growth hormone, interleukin-6 and glucocorticoids mRNA analysis and promoter characterization.

  2000cites this paper
• Hormonal regulation of physiological cell turnover and apoptosis

  2000cites this paper
• Transcriptional potencies of inhaled glucocorticoids.

  2000influential citation
• Molecular basis for corticosteroid action in asthma.

  2000cites this paper
• Role of canonical glucocorticoid responsive elements in modulating expression of genes regulated by the arylhydrocarbon receptor.

  1999cites this paper
• Mechanisms of Glucocorticoid-receptor-mediated Repression of Gene Expression.

  1999cites this paper
• The promoter region of human prepro-nociceptin gene and its regulation by cyclic AMP and steroid hormones.

  1999cites this paper
• [Mechanism of action of glucocorticoids in asthma].

  1999cites this paper
• DNA sequence-specific recognition by the Saccharomyces cerevisiae "TATA" binding protein: promoter-dependent differences in the thermodynamics and kinetics of binding.

  1998cites this paper
• Osteoprogenitor cells in cell populations derived from mouse and rat calvaria differ in their response to corticosterone, cortisol, and cortisone.

  1998cites this paper