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Multiscale photoacoustic imaging without motion using single‐pixel imaging

Xianlin Song,Xiaohai Yu,Rui Wang,Ganyu Chen,Jiahao Zeng

Published 2021 in Journal of Biophotonics

ABSTRACT

The conventional photoacoustic microscopy usually uses mechanical raster scanning to obtain three‐dimensional information, and the imaging speed is limited. Meanwhile, the conventional photoacoustic microscopy can only be performed at one single scale due to fixed resolution, it cannot make full use of multiscale information for integrated imaging. Here, we proposed a multiscale photoacoustic microscopy based on single‐pixel imaging. A sequence of sinusoidal fringes with varying spatial frequencies is used to obtain the Fourier coefficients in the case of a single ultrasonic transducer. By controlling the spatial frequency of fringe, the acquisition of Fourier coefficients can be controlled and multiscale imaging can be achieved. The feasibility of this method is verified by theory and simulation. The results show that the lateral resolution can be tuned from several microns to tens of microns without mechanical scanning. This method will expand the application of photoacoustic imaging in biomedical research.

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