Multiple Origin and Tumor Heterogeneity of Prostatic Ductal Adenocarcinoma in the Han Chinese Population

Purpose: Prostatic ductal adenocarcinoma (PDA) is recognized as an advanced stage cancer and is often observed in conjunction with acinar adenocarcinoma, with abundant cytoplasm arranged in a papillary pattern. When compared with acinar adenocarcinoma, it is characterized by an increased biochemical recurrence rate and unusual metastasis sites. The purpose of the present study was to further elucidate the genomic alterations associated with PDA. Methods: Whole-exome sequencing (WES) sequencing and linkage analyses were performed on genomic DNAs isolated from formalin-fixed, paraffin-embedded (FFPE) specimens obtained from paired tumor and benign tissues from 11 PDA cases. The profiles of somatic mutations, indels as well as copy-number alterations were characterized in the tumor tissues using the benign sections as controls. The mutational spectra of the 11 PDA cases were compared with the data obtained from the Cancer Genome Atlas (TCGA) for eight primary prostatic acinar adenocarcinoma patients. Results: The same somatic changes were observed in PDA as in advanced and/or metastatic acinar adenocarcinomas. For example, the mutations in a known prostate cancer driver gene CDKN1A, were the most significant events among 17% of tumors. In addition to the known amplification of chromosomes 1q, 4p, 8q, and 14q, the copy number of several additional regions were also significantly increased. The origin of PDA was heterogeneous: some patients (e.g. P5) were consistent with the monoclonal model, while others (e.g. P7) were polyclonal. Conclusions: PDA has a similar mutation profile compared to AAC of the prostate with high Gleason scores have similar mutational profiles. The somatic mutations in PDA may be the reason for its invasive biological behavior.

• Publication year

  2021

• Venue

  Genetic Testing and Molecular Biomarkers

• Publication date

  2021-11-01

• Fields of study

  Medicine

• Identifiers
  DOI 10.1089/gtmb.2021.0126PMID 34788144
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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