Resistance to pydiflumetofen in Botrytis cinerea: risk assessment and detection of point mutations in sdh genes that confer resistance.

BACKGROUND Gray mold caused by Botrytis cinerea Pers. is one of the most significant airborne diseases. It is capable of infesting a wide range of crops, causing significant losses in yield and quality worldwide. Pydiflumetofen, which is one of a new generation of succinate dehydrogenase inhibitors, is currently being registered in China for the control of gray mold in a variety of crops. Baseline sensitivity, resistance risk assessment, and resistant mechanism of Botrytis cinerea to pydiflumetofen was undertaken in this study. RESULTS A total of 138 strains of B. cinerea from 10 different regions were tested for their sensitivity to pydiflumetofen, and the mean EC50 value was 0.0056 μg mL-1 . Eight mutants were obtained by fungicide adaption from five sensitive parental isolates, and the resistance factor (RF) ranged from 51 to 135. The mutants exhibited strong adaptive traits in conidial production, conidial germination, and pathogenicity. Positive cross-resistance was only observed between other SDHIs (i.e. boscalid, fluopyram, and isopyrazam). Two different types of pydiflumetofen-resistant mutants were identified: point mutation P225L in sdhB, double mutation G85A and I93V in sdhC. In vivo control efficacy of pydiflumetofen on the resistant mutants carrying P225L in sdhB, as well as G85A and I93V in sdhC was significantly decreased to 52.62% and 32.27%, respectively. CONCLUSION The fitness was significantly higher for all pydiflumetofen-resistant mutants than for the corresponding parental. Two types point mutations, sdhB-P225L, sdhC-G85A and I93V, might confer resistance to pydiflumetofen in B. cinerea. A precautionary resistance management strategy should be implemented. This article is protected by copyright. All rights reserved.

• Publication year

  2021

• Venue

  Pest Management Science

• Publication date

  2021-12-19

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1002/ps.6762PMID 34927349
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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