Effects of Engineered Saccharomyces cerevisiae Fermenting Cellobiose through Low-Energy-Consuming Phosphorolytic Pathway in Simultaneous Saccharification and Fermentation

Until recently, four types of cellobiose-fermenting Saccharomyces cerevisiae strains have been developed by introduction of a cellobiose metabolic pathway based on either intracellular β-glucosidase (GH1-1) or cellobiose phosphorylase (CBP), along with either an energy-consuming active cellodextrin transporter (CDT-1) or a non-energy-consuming passive cellodextrin facilitator (CDT-2). In this study, the ethanol production performance of two cellobiose-fermenting S. cerevisiae strains expressing mutant CDT-2 (N306I) with GH1-1 or CBP were compared with two cellobiose-fermenting S. cerevisiae strains expressing mutant CDT-1 (F213L) with GH1-1 or CBP in the simultaneous saccharification and fermentation (SSF) of cellulose under various conditions. It was found that, regardless of the SSF conditions, the phosphorolytic cellobiose-fermenting S. cerevisiae expressing mutant CDT-2 with CBP showed the best ethanol production among the four strains. In addition, during SSF contaminated by lactic acid bacteria, the phosphorolytic cellobiose-fermenting S. cerevisiae expressing mutant CDT-2 with CBP showed the highest ethanol production and the lowest lactate formation compared with those of other strains, such as the hydrolytic cellobiose-fermenting S. cerevisiae expressing mutant CDT-1 with GH1-1, and the glucose-fermenting S. cerevisiae with extracellular β-glucosidase. These results suggest that the cellobiose-fermenting yeast strain exhibiting low energy consumption can enhance the efficiency of the SSF of cellulosic biomass.

• Publication year

  2021

• Venue

  Journal of Microbiology and Biotechnology

• Publication date

  2021-12-09

• Fields of study

  Medicine, Engineering, Environmental Science

• Identifiers
  DOI 10.4014/jmb.2111.11047PMID 34949751PMCID 9628822
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Comparative genomics of Lactobacillus fermentum suggests a free-living lifestyle of this lactic acid bacterial species.

  2020cited by this paper
• Gentiobiose and cellobiose content in fresh and fermenting cucumbers and utilization of such disaccharides by lactic acid bacteria in fermented cucumber juice medium

  2020cited by this paper
• Cellulosic ethanol production: Progress, challenges and strategies for solutions.

  2019cited by this paper
• Enhanced cellobiose fermentation by engineered Saccharomyces cerevisiae expressing a mutant cellodextrin facilitator and cellobiose phosphorylase.

  2018cited by this paper
• Yeast Derived LysA2 Can Control Bacterial Contamination in Ethanol Fermentation

  2018cited by this paper
• Metabolic engineering of yeast for lignocellulosic biofuel production.

  2017cited by this paper
• Intracellular cellobiose metabolism and its applications in lignocellulose-based biorefineries.

  2017cited by this paper
• Improved ethanol production by engineered Saccharomyces cerevisiae expressing a mutated cellobiose transporter during simultaneous saccharification and fermentation.

  2017cited by this paper
• Transporter engineering for cellobiose fermentation under lower pH conditions by engineered Saccharomyces cerevisiae.

  2017cited by this paper
• Evaluation of Ethanol Production Activity by Engineered Saccharomyces cerevisiae Fermenting Cellobiose through the Phosphorolytic Pathway in Simultaneous Saccharification and Fermentation of Cellulose.

  2017cited by this paper
• Bacteriophage application restores ethanol fermentation characteristics disrupted by Lactobacillusfermentum

  2015cited by this paper
• Energetic benefits and rapid cellobiose fermentation by Saccharomyces cerevisiae expressing cellobiose phosphorylase and mutant cellodextrin transporters.

  2013cited by this paper
• Bacteriophage-encoded lytic enzymes control growth of contaminating Lactobacillus found in fuel ethanol fermentations

  2013cited by this paper
• Analysis of cellodextrin transporters from Neurospora crassa in Saccharomyces cerevisiae for cellobiose fermentation

  2013cited by this paper
• Simultaneous saccharification and fermentation by engineered Saccharomyces cerevisiae without supplementing extracellular β-glucosidase.

  2013cited by this paper
• Competition between pentoses and glucose during uptake and catabolism in recombinant Saccharomyces cerevisiae

  2012cited by this paper
• Simultaneous co-fermentation of mixed sugars: a promising strategy for producing cellulosic ethanol.

  2012cited by this paper
• Cofermentation of Cellobiose and Galactose by an Engineered Saccharomyces cerevisiae Strain

  2011cited by this paper
• Microbial contamination of fuel ethanol fermentations

  2011cited by this paper
• Resistance of yeasts to weak organic acid food preservatives.

  2011cited by this paper
• Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation

  2010cited by this paper
• Cellodextrin Transport in Yeast for Improved Biofuel Production

  2010cited by this paper
• Diversity of lactic acid bacteria of the bioethanol process

  2010cited by this paper
• Modeling bacterial contamination of fuel ethanol fermentation

  2009cited by this paper
• A dominant mutation that alters the regulation of INO1 expression in Saccharomyces cerevisiae.

  1992cited by this paper
• Thermodynamics of hydrolysis of disaccharides. Cellobiose, gentiobiose, isomaltose, and maltose.

  1989cited by this paper
• CHARACTERISTICS OF CELLOBIOSE PHOSPHORYLASE

  1961cited by this paper

• Recent progress in engineering yeast producers of cellulosic ethanol

  2025influential citation
• Engineering of Ogataea polymorpha strains with ability for high-temperature alcoholic fermentation of cellobiose

  2024cites this paper
• Saccharomyces cerevisiae cell surface display technology: Strategies for improvement and applications

  2022cites this paper