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Nitrogen nutrition modifies the susceptibility of Arabidopsis thaliana to the necrotrophic fungus, Alternaria brassicicola.

Thibault Barrit,A. Porcher,Caroline Cukier,P. Satour,T. Guillemette,A. Limami,B. Teulat,C. Campion,E. Planchet

Published 2022 in Physiologia Plantarum : An International Journal for Plant Biology

ABSTRACT

The impact of the form of nitrogen (N) source (nitrate versus ammonium) on the susceptibility to Alternaria brassicicola, a necrotrophic fungus, has been examined in Arabidopsis thaliana at the rosette stage. Nitrate nutrition was found to increase fungal lesions considerably. There was a similar induction of defence gene expression following infection under both N nutritions, except for the phytoalexin deficient 3 gene, which was overexpressed with nitrate. Nitrate also led to a greater nitric oxide production occurring in planta during the saprophytic growth and lower nitrate reductase (NIA1) expression 7 days after inoculation. This suggests that nitrate reductase-dependent nitric oxide production had a dual role, whereby, despite its known role in the generic response to pathogens, it affected plant metabolism, and this facilitated fungal infection. In ammonium-grown plants, infection with A. brassicicola induced a stronger gene expression of ammonium transporters and significantly reduced the initially high ammonium content in the leaves. There was a significant interaction between N source and inoculation (presence versus absence of the fungus) on the total amino acid content, while N nutrition reconfigured the spectrum of major amino acids. Typically, a higher content of total amino acid, mainly due to a stronger increase in asparagine and glutamine, is observed under ammonium nutrition while, in nitrate-fed plants, glutamate was the only amino acid which content increased significantly after fungal inoculation. N nutrition thus appears to control fungal infection via a complex set of signalling and nutritional events, shedding light on how nitrate availability can modulate disease susceptibility.

PUBLICATION RECORD

  • Publication year

    2022

  • Venue

    Physiologia Plantarum : An International Journal for Plant Biology

  • Publication date

    2022-01-06

  • Fields of study

    Biology, Medicine, Environmental Science

  • Identifiers
    DOI 10.1111/ppl.13621PMID 34989007
  • External record

    Open on Semantic Scholar

  • Source metadata

    Semantic Scholar, PubMed

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