Presence and localization of apelin and its cognate receptor in the testis of the dog using immunohistochemical and molecular biology technique.

Apelin, a member of the adipokine family, is a new endogenous peptide and it’s involved, by interacting with a specific receptor, in the control of human and laboratory animals’male reproduction. As far as we know, no data is available about the presence of the apelinergic system in dog testicles. Therefore the aim of this study was to show, for the first time, its presence and distribution in the canine testis by immunoistochemistry and molecular biology studies. For this purpose, five fertile and healthy male dogs were used and subjected to elective orchiectomy. The immunohistochemical reaction evidenced the presence of apelin and its receptor in the canine testis. In particular, apelin appeared localized in spermatids and spermatozoa with a positive signal in the “acrosomal bodies”. Regarding to the apelin receptor, a positive immunoreaction was evidenced in the cytoplasm of the cells localized in the basal portion of the seminiferous tubules, likely Sertoli’s cells, and in the cytoplasm of Leydig cells. The RT-PCR analysis showed the presence of transcripts for apelin and apelin receptor in all the samples examined. In conclusion our result allows us to hypothesize that the role of the paracrine and endocrine apelinergic system reported in laboratory animals can also be found in the dog.

• Publication year

  2022

• Venue

  Unknown venue

• Publication date

  2022-01-21

• Fields of study

  Not labeled

• Identifiers
  DOI 10.21203/rs.3.rs-1266418/v1
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

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