Supplemental dietary zinc sulfate and folic acid combination improves testicular volume and hemodynamics, testosterone levels and semen quality in rams under heat stress conditions.

This study was aimed to investigate the combined effect of zinc sulfate and folic acid (ZnF) dietary supplementation on testicular hemodynamics (TH), testicular volume (TV), serum testosterone levels (T) and semen quality of rams under heat stress conditions. Fifteen Ossimi rams were allocated to three groups: (1) G0 (n=5) received only basic diet; (2) G1 (n=5) received basic diet + ZnF (Zn, 0.4 mg/kg bw; F, 0.02 mg/kg bw) and (3) G2 (n=5) received basic diet + ZnF (Zn, 0.8 mg/kg bw; F, 0.04 mg/kg bw) daily for 60 days. TH was evaluated using color (testicular coloration, TC) and spectral modes [resistive index (RI) and pulsatility index (PI)] Doppler of the supra testicular arteries (proximal and distal parts, STA). Semen traits including progressive motility (PM), alive sperm % (AS), sperm viability (SV), sperm abnormalities (SA) and acrosome integrity (AI) were also assessed. The examinations were carried out one month before (D -30), the beginning of ZnF inclusion in the diet (D 0), and continued for the successive two months (D 30 and D 60). TH was significantly (P < 0.05) improved at D 30 and D 60, evidenced by lowering both RI and PI and increasing of TC in G1 compared to G0 and G2. In addition, both TV and serum T levels were elevated (P < 0.05) at D 30 and D 60 in G1 compared to other groups. Semen quality parameters (PM, AS, SV and AI) were significantly (P < 0.05) augmented in the same trend as TH, TV and T in G1 versus G0 and G2. A marked decrease (P < 0.05) in SA % was noticed at Days 30 and 60 after ZnF inclusion in G1 compared to G0 and G2. In conclusion, supplementation of the summer diet with ZnF improved the whole reproductive functions such as testicular hemodynamics and semen quality of rams housed in heat stress conditions.

• Publication year

  2022

• Venue

  Reproduction in domestic animals = Zuchthygiene

• Publication date

  2022-02-11

• Fields of study

  Agricultural and Food Sciences, Medicine

• Identifiers
  DOI 10.1111/rda.14096PMID 35147249
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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