Optimizing the transient transfection process of HEK-293 suspension cells for protein production by nucleotide ratio monitoring

No abstract is available for this paper.

• Publication year

  2014

• Venue

  Cytotechnology (Dordrecht)

• Publication date

  2014-05-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1007/s10616-013-9601-3PMID 23775287PMCID PMC3973801
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions

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• Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems

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• Recombinant protein production by large-scale transient gene expression in mammalian cells: state of the art and future perspectives

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• Control of Culture Environment for Improved Polyethylenimine‐Mediated Transient Production of Recombinant Monoclonal Antibodies by CHO Cells

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• Serum-free suspensin large-scale transient transfection of CHO cells in WAVE bioreactors

  2006cited by this paper
• Large-Scale transfection of mammalian cells for the fast production of recombinant protein

  2006cited by this paper
• A two-stage poly(ethylenimine)-mediated cytotoxicity: implications for gene transfer/therapy.

  2005cited by this paper
• Large-scale Transient Transfection of Mammalian Cells: A Newly Emerging Attractive Option for Recombinant Protein Production

  2005cited by this paper
• TRANSIENT GENE EXPRESSION FOR RAPID PROTEIN PRODUCTION: STUDIES & OPTIMIZATIONS UNDER SERUM-FREE CONDITIONS

  2005cited by this paper
• Large-scale transient expression of therapeutic proteins in mammalian cells.

  2005cited by this paper
• Transient gene expression in HEK293 cells: peptone addition posttransfection improves recombinant protein synthesis.

  2005cited by this paper
• Evaluation of anchorage-dependent cell propagation systems for production of human acetylcholinesterase by recombinant 293 cells

  2004cited by this paper
• Cultural and physiological factors affecting expression of recombinant proteins

  2004cited by this paper
• Effects of temperature shift on cell cycle, apoptosis and nucleotide pools in CHO cell batch cultues

  2004cited by this paper
• TubeSpin satellites: a fast track approach for process development with animal cells using shaking technology

  2004cited by this paper
• Serum‐free large‐scale transient transfection of CHO cells

  2004cited by this paper
• Calfection: a novel gene transfer method for suspension cells.

  2004cited by this paper
• 100-liter transient transfection

  2004cited by this paper
• Calcium-phosphate mediated DNA transfer into HEK-293 cells in suspension: control of physicochemical parameters allows transfection in stirred media. Transfection and protein expression in mammalian cells

  2004cited by this paper
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  2004cited by this paper
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  2003cited by this paper
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  2003cited by this paper
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  2003cited by this paper
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  2002cited by this paper
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  2002cited by this paper
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  2002cited by this paper
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  2002cited by this paper
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  2001cited by this paper
• Trypan Blue Exclusion Test of Cell Viability

  2001cited by this paper
• Characterization of commercially available and synthesized polyethylenimines for gene delivery.

  2000cited by this paper
• Rapid crossing of the pulmonary endothelial barrier by polyethylenimine/DNA complexes

  2000cited by this paper
• A Comparison of Different Methods To Determine the End of Exponential Growth in CHO Cell Cultures for Optimization of Scale‐Up

  2000cited by this paper
• Transient gene expression in mammalian cells grown in serum-free suspension culture

  1999influential reference
• Monitoring of intracellular ribonucleotide pools is a powerful tool in the development and characterization of mammalian cell culture processes.

  1999cited by this paper
• Comparison between cationic polymers and lipids in mediating systemic gene delivery to the lungs

  1999cited by this paper
• Large-scale transient expression in mammalian cells for recombinant protein production.

  1999cited by this paper
• Tracking the intracellular path of poly(ethylenimine)/DNA complexes for gene delivery.

  1999influential reference
• The relationship between intracellular UDP-N-acetyl hexosamine nucleotide pool and monoclonal antibody production in a mouse hybridoma.

  1998cited by this paper
• The size of DNA/transferrin-PEI complexes is an important factor for gene expression in cultured cells

  1998cited by this paper
• Polyethylenimine-based intravenous delivery of transgenes to mouse lung

  1998cited by this paper
• Serum-free production of recombinant proteins and adenoviral vectors by 293SF-3F6 cells.

  1998cited by this paper
• Intracellular UDP−N‐Acetylhexosamine Pool Affects N‐Glycan Complexity: A Mechanism of Ammonium Action on Protein Glycosylation

  1998influential reference
• ExGen 500 is an efficient vector for gene delivery to lung epithelial cells in vitro and in vivo

  1997cited by this paper
• Nonviral Gene Delivery to the Rat Kidney with Polyethylenimine

  1997cited by this paper
• Serum-dependent and cell cycle-dependent expression from a cytomegalovirus-based mammalian expression vector.

  1997cited by this paper
• Polyethylenimine (PEI) is a simple, inexpensive and effective reagent for condensing and linking plasmid DNA to adenovirus for gene delivery

  1997cited by this paper
• Polyethylenimine-mediated DNA transfection of peripheral and central neurons in primary culture: probing Ca2+ channel structure and function with antisense oligonucleotides.

  1996cited by this paper
• Optimized galenics improve in vitro gene transfer with cationic molecules up to 1000-fold.

  1996cited by this paper
• A Powerful Nonviral Vector for In Vivo Gene Transfer into the Adult Mammalian Brain: Polyethylenimine

  1996cited by this paper
• A COS-cell-based system for rapid production and quantification of scFv::IgC kappa antibody fragments.

  1995cited by this paper
• A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine.

  1995cited by this paper
• Efficient gene transfer into human hepatocytes by baculovirus vectors.

  1995cited by this paper
• Evaluation of the proteolytic potential of in vitro-cultivated hybridoma and recombinant mammalian cells.

  1994cited by this paper
• Biochemistry of growth inhibition by ammonium ions in mammalian cells

  1994cited by this paper
• High‐Efficiency Gene Transfer Mediated by Adenovirus‐Polylysine‐DNA Complexes

  1994cited by this paper
• High-level expression of secreted proteins from cells adapted to serum-free suspension culture.

  1993cited by this paper
• Evaluation of production of recombinant human interleukin‐2 in fluidized bed bioreactor

  1992cited by this paper
• Intracellular ribonucleotide pools as a tool for monitoring the physiological state of in vitro cultivated mammalian cells during production processes

  1992influential reference
• INTRACELLULAR CONCENTRATION OF ATP AND OTHER NUCLEOTIDES DURING CONTINUOUS CULTIVATION OF HYBRIDOMA CELLS

  1991cited by this paper
• Improved ion-pair high-performance liquid chromatographic method for the quantification of a wide variety of nucleotides and sugar-nucleotides in animal cells.

  1991cited by this paper
• Production of recombinant human interleukin-2 with BHK cells in a hollow fibre and a stirred tank reactor with protein-free medium.

  1990cited by this paper
• Quantitation of protein.

  1990cited by this paper
• Monoclonal antibodies: production and application.

  1989cited by this paper
• Serum-free media used for cultivation of hybridomas.

  1989cited by this paper
• Calcium phosphate-mediated gene transfer: a highly efficient transfection system for stably transforming cells with plasmid DNA.

  1988cited by this paper
• Human erythropoietin gene: high level expression in stably transfected mammalian cells and chromosome localization.

  1986cited by this paper
• A Method for Quantitative Amino Acid Analysis Using Precolumn o-Phthalaldehyde Derivatization and High Performance Liquid Chromatography

  1981cited by this paper
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• Regulation of enzyme function.

  1969cited by this paper
• Lactic Dehydrogenase Activity in Blood.∗

  1955cited by this paper
• The measurement of proliferation in tissue cultures by enumeration of cell nuclei.

  1951cited by this paper

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