Alternative oxidase plays a role in minimizing ROS and RNS produced under salinity stress in Arabidopsis thaliana.

Under stress conditions, the overproduction of different reactive oxygen species (ROS) and reactive nitrogen species (RNS) cause imbalance in the redox homeostasis of the cell leading to nitro-oxidative stress in plants. Alternative oxidase (AOX) is a conserving terminal oxidase of the mitochondrial electron transport chain, which can minimize the ROS. Still, the role of AOX in regulation of RNS during nitro-oxidative stress imposed by salinity stress is not known. Here, we investigated the role of AOX in minimizing ROS and RNS induced by 150 mM NaCl in Arabidopsis using transgenic plants overexpressing (AOX OE) and antisense lines (AOX AS) of AOX. Imposing NaCl treatment leads to a 4-fold enhanced expression of AOX accompanied by enhanced AOX capacity in WT Col-0. Further AOX-OE seedlings displayed enhanced growth compared to the AOX-AS line under stress. Examination of NO levels by DAF-FM fluorescence and chemiluminescence revealed that AOX overexpression leads to reduced levels of NO. The total NR activity was elevated under NaCl, but no significant change was observed in WT, AOX OE and AS lines. The total ROS, superoxide, H2 O2 levels and lipid peroxidation were higher in the AOX-AS line than WT and AOX-OE lines. The peroxynitrite levels were also higher in the AOX-AS line than in WT and AOX-OE lines; further, the expression of antioxidant genes was elevated in AOX-AS. Taken together, our results suggest that AOX play an important role in the mitigation of ROS and RNS levels and enhance plant growth, thus providing tolerance against nitro-oxidative stress exerted by NaCl.

• Publication year

  2022

• Venue

  Physiologia Plantarum : An International Journal for Plant Biology

• Publication date

  2022-02-12

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1111/ppl.13649PMID 35149995
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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