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Determination of Molecular Interactions Between Fraser’s Photinia (Photinia X Fraseri) and Its Endophytic Bacterium PGB_Invit

Nil Türkölmez,Merve Karakaya,Hamit Ekinci,S. Lucas,Özlem Akkaya,M. G. Şeker,Ceyhun Kayıhan,Y. Ozden

Published 2022 in Unknown venue

ABSTRACT

An endophytic beneficial bacterium, was isolated and characterized from in vitro grown microshoots of Fraser’s Photinia in our previous studies. This bacterium, nominated as PGB_invit (Pseudorhodoplanes sp.), has ability to fix nitrogen and produce plant growth promoting regulators such as indole acetic acid and gibberellic acid. Probably because of all of these features, this specific bacterium promotes in vitro proliferation of Fraser’s Photinia microshoots and enables microshoots to be conserved at 25 ºC for up to 16 months without requiring periodic subculture. Due to its beneficial influence on growth of Fraser’s Photinia, it is aimed to identify physiological, biochemical and molecular influences of this bacterium together with determination of its the localization in plant tissues. Our results showed that inoculation with PGB_invit bacteria (106 cfu/ml) has increased chlorophyll content without causing any severe oxidative stress. Moreover, the genes [gene bank ID XP_008352422.1 (predicted “MID1-Complementing Activity 1”), PWZ54828.1 (Ubiquitin conjugating enzyme E2), XP_028948963.1 (predicted “E3 ubiquitin-protein ligase RHA1B-like”), XP_008339965.2 (uncharacterized protein), XP_028948163.1 (4-coumarate--CoA ligase-like 9), and XP_028953023 (methionine aminopeptidase 2B-like)] which are upregulated or downregulated after inoculation of this bacterium according to RNA-seq analysis, were also confirmed with qRT-PCR in order to understand its molecular interaction with its host.

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