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Evaluation of pleotropic protective activity of Capparis spinosa extract on arthritis rat model.

Mohammed Yosri,Hanna Y. Ahmeda,B. Amin,M. M. Elaassera,M. A. Aziz

Published 2022 in Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry

ABSTRACT

BACKGROUND Capparis spinosa grow in Asian and Mediterranean desert areas. Different parts of Capparis spinosa including flowers have been used in different applications of folk medicine. OBJECTIVE The objective of this study is to evaluate the anti-arthritic potential of ethanolic extract of Egyptian Capparis spinosa flowers in a rat model of rheumatoid arthritis. METHODS Capparis spinosa flowers was extracted and testing its in vitro anti-inflammatory activity. Capparis spinosa flowers extract was analyzed using LC-qTOF-MS/MS. Extract were in vivo tested where animals were split into six groups: negative control group, induced arthritic animals, arthritic rats receiving 7, 14 and 28 mg /kg of Capparis spinosa extract respectively in three groups to detect optimum dose and the induced group receiving a standard drug. Arthritic score was checked daily for 15 days after induction. After sacrificing of animals, joints and muscles were subjected to microscopic and ultra-structure examinations. Ex vivo culturing of osteoclasts was performed. Cytokines levels were measured in all examined groups. RESULTS Capparis spinosa flowers extract has a promising in vitro anti-inflammatory activity. LC-qTOF-MS/MS analysis revealed the presence of combined compounds of three different groups. The in vivo results revealed that 7 mg/kg of Capparis spinosa extract was the optimal dose which decreased inflammation signs through controlling chondrocytes, osteoclast and levels of inflammatory mediators. CONCLUSION LC-Mass analysis revealed that, Capparis spinosa extract contained a mixture of flavonol glycolsides, flavan-3-ols and hydroxycinnamic acid derivatives which may have beneficial multifunction in regulating arthritic symptoms.

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