How advances in cryo-electron tomography have contributed to our current view of bacterial cell biology

Highlights • Automation led to the localization of FtsZ filaments and constriction mechanism.• CEMOVIS confirmed the presence of the mycoloic-acid outer membrane in Mycobacteria.• Cryo-FIB SEM illustrated the sporulation process and asymmetrical cell division.• Cryo-CLEM was used for the identification of a non-canonical T6SS.• Subtomogram averaging led to higher resolution by overcoming missing information reveals internal conformation changes and mechanisms.• Perspective of further coming advancements.

• Publication year

  2022

• Venue

  Journal of structural biology: X

• Publication date

  2022-02-01

• Fields of study

  Biology, Medicine, Materials Science

• Identifiers
  DOI 10.1016/j.yjsbx.2022.100065PMID 35252838PMCID 8894267
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  2019cited by this paper
• Teichoic acids anchor distinct cell wall lamellae in an apically growing bacterium

  2019cited by this paper
• [Cryo-Electron Microscopy].

  2018cited by this paper
• Big data in cryoEM: automated collection, processing and accessibility of EM data.

  2018cited by this paper
• Advances in cryo-electron tomography for biology and medicine.

  2018cited by this paper
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• In situ and high-resolution Cryo-EM structure of the Type VI secretion membrane complex

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• Oxygen plasma focused ion beam scanning electron microscopy for biological samples

  2018cited by this paper
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  2018cited by this paper
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• Functional insights into pathogen biology from 3D electron microscopy

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• Short FtsZ filaments can drive asymmetric cell envelope constriction at the onset of bacterial cytokinesis

  2017cited by this paper
• Structure of the hexagonal surface layer on Caulobacter crescentus cells

  2017cited by this paper
• An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology

  2016cited by this paper
• EMPIAR: a public archive for raw electron microscopy image data

  2016cited by this paper
• Direct Electron Detectors.

  2016cited by this paper
• Electron cryotomography of vitrified cells with a Volta phase plate.

  2015cited by this paper
• Structural analysis of multicellular organisms with cryo-electron tomography

  2015cited by this paper
• Architecture of the ring formed by the tubulin homologue FtsZ in bacterial cell division

  2014cited by this paper
• Cryo-electron microscopy of vitreous sections.

  2014cited by this paper
• Zernike phase contrast cryo-electron tomography of whole bacterial cells.

  2014cited by this paper
• Correlated cryogenic photoactivated localization microscopy and electron cryotomography

  2014cited by this paper
• Peptidoglycan transformations during Bacillus subtilis sporulation

  2013cited by this paper
• The mobility of two kinase domains in the Escherichia coli chemoreceptor array varies with signalling state

  2013cited by this paper
• Bacterial chemoreceptor arrays are hexagonally packed trimers of receptor dimers networked by rings of kinase and coupling proteins

  2012cited by this paper
• General protein diffusion barriers create compartments within bacterial cells.

  2012cited by this paper
• PyTom: a python-based toolbox for localization of macromolecules in cryo-electron tomograms and subtomogram analysis.

  2012cited by this paper
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  2012cited by this paper
• Molecular architecture of chemoreceptor arrays revealed by cryoelectron tomography of Escherichia coli minicells

  2012cited by this paper
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  2011cited by this paper
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  2011cited by this paper
• EMDataBank.org: unified data resource for CryoEM

  2010cited by this paper
• The metabolic enzyme CTP synthase forms cytoskeletal filaments

  2010cited by this paper
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  2010cited by this paper
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  2009cited by this paper
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  2009cited by this paper
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• UCSF tomography: an integrated software suite for real-time electron microscopic tomographic data collection, alignment, and reconstruction.

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• Electron energy filtering significantly improves amplitude contrast of frozen-hydrated protein at 300kV.

  2006cited by this paper
• Automated electron microscope tomography using robust prediction of specimen movements.

  2005cited by this paper
• Cutting artefacts and cutting process in vitreous sections for cryo-electron microscopy.

  2005cited by this paper
• Structural studies by electron tomography: from cells to molecules.

  2005cited by this paper
• TOM software toolbox: acquisition and analysis for electron tomography.

  2005cited by this paper
• Cryo‐electron microscopy of vitreous sections

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• Pyrodictium cannulae enter the periplasmic space but do not enter the cytoplasm, as revealed by cryo-electron tomography.

  2003cited by this paper
• Electron tomography of ice-embedded prokaryotic cells.

  1998cited by this paper
• Computer visualization of three-dimensional image data using IMOD.

  1996cited by this paper
• The EM Program Package: A Platform for Image Processing in Biological Electron Microscopy

  1996cited by this paper
• Double-tilt electron tomography.

  1995cited by this paper
• FtsZ ring structure associated with division in Escherichia coli

  1991cited by this paper
• Cryo-electron microscopy of vitrified specimens

  1988cited by this paper
• Theory and practice of high pressure freezing.

  1987cited by this paper
• Bacterial mesosomes: Method dependent artifacts

  1981cited by this paper
• The ultrastructure of cell membranes and their derivatives.

  1959cited by this paper
• ELECTRON MICROSCOPY OF ULTRA-THIN SECTIONS OF BACTERIA

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• EMDB—the Electron Microscopy Data Bank

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• A dynamic duo: Understanding the roles of FtsZ and FtsA for Escherichia coli cell division through in vitro approaches.

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• Revealing bacterial cell biology using cryo-electron tomography.

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• Mechanisms of E. coli chemotaxis signaling pathways visualized using cryoET and computational approaches

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• Binding and Functional Folding (BFF): A Physiological Framework for Studying Biomolecular Interactions and Allostery

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