Extraction & Analysis of Algal Pigments by Thin Layer Chromotography & Spectrophotometric Analysis

The study focuses to extract and analyse the algal pigments present in the lithophilic algae collected from various sites in the concrete walls of Department of Biotechnology, University of Madras. The samples collected were stored in sterile plastic ware and cultured in a growth medium in the laboratory for further analysis. Significant algal growth was observed in media enriched with CaCo3.Microscopic observations reveals filamentous algae of the family Cyanobacteria. The cultured algal biomass was treated with solvent and the pigments were extracted. The pigment extracts were analysed using two-dimension thin layer chromatography (TLC) and confirmed using UV-Vis Spectrophotometer. The filamentous algae have significant amount of Chlorophyll a &b and Carotenoids.

• Publication year

  2022

• Venue

  International Journal of Current Science Research and Review

• Publication date

  2022-03-21

• Fields of study

  Not labeled

• Identifiers
  DOI 10.47191/ijcsrr/v5-i3-20
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Photosynthetic pigments, lipids and phenolic compounds of three green algae isolated from freshwater ecosystem

  2020cited by this paper
• Isolation of chlorophylls and carotenoids from freshwater algae using different extraction methods

  2018cited by this paper
• Biomass and Pigments of Benthic Algae

  2017cited by this paper
• Column chromatography as a useful step in purification of diatom pigments.

  2016cited by this paper
• Review on the influence of biological deterioration on the surface properties of building materials: Organisms, materials, and methods

  2015cited by this paper
• Techniques for extraction of bioactive compounds from plant materials: A review

  2013cited by this paper
• Commercial and industrial applications of micro algae - A review

  2012cited by this paper
• Carotenoids in Algae: Distributions, Biosyntheses and Functions

  2011cited by this paper
• Biodeterioration of Coastal Concrete Structures by Marine Green Algae

  2010cited by this paper
• EVALUATION OF PIGMENT EXTRACTION METHODS AND A RECOMMENDED PROTOCOL FOR PERIPHYTON CHLOROPHYLL a DETERMINATION AND CHEMOTAXONOMIC ASSESSMENT 1

  2006cited by this paper
• Extraction of pigments and fatty acids from the green alga Scenedesmus obliquus (Chlorophyceae)

  2000cited by this paper
• Lithophytic algae: A major threat to the karst formation of show caves

  2000cited by this paper
• Extraction and quantification of chlorophyll a from freshwater green algae

  1998cited by this paper
• Photosynthetic pigments of algae

  1990cited by this paper
• Profiles of photosynthetic pigments in the ocean using thin-layer chromatography

  1974cited by this paper

• No citing papers are available for this paper.