High-throughput functional evaluation of human cancer-associated mutations using base editors

No abstract is available for this paper.

• Publication year

  2022

• Venue

  Nature Biotechnology

• Publication date

  2022-04-11

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1038/s41587-022-01276-4PMID 35411116PMCID PMC10243181
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Base editing sensor libraries for high-throughput engineering and functional analysis of cancer-associated single nucleotide variants

  2022cited by this paper
• Functional interrogation of DNA damage response variants with base editing screens.

  2021cited by this paper
• Predicting the efficiency of prime editing guide RNAs in human cells

  2020cited by this paper
• CRISPR C-to-G base editors for inducing targeted DNA transversions in human cells

  2020cited by this paper
• Glycosylase base editors enable C-to-A and C-to-G base changes

  2020cited by this paper
• Pan-cancer analysis of whole genomes

  2020cited by this paper
• Sequence-specific prediction of the efficiencies of adenine and cytosine base editors

  2020cited by this paper
• Genome-wide interrogation of gene functions through base editor screens empowered by barcoded sgRNAs

  2020cited by this paper
• Determinants of Base Editing Outcomes from Target Library Analysis and Machine Learning.

  2020cited by this paper
• Prediction of the sequence-specific cleavage activity of Cas9 variants

  2020cited by this paper
• Massively parallel assessment of human variants with base editor screens

  2020cited by this paper
• Multiplexed measurement of variant abundance and activity reveals VKOR topology, active site and human variant impact

  2020cited by this paper
• Directed evolution of adenine base editors with increased activity and therapeutic application

  2020cited by this paper
• Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants

  2020cited by this paper
• HPV-mediated nuclear export of HP1γ drives cervical tumorigenesis by downregulation of p53

  2020cited by this paper
• Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity

  2020cited by this paper
• Comprehensive assessment of computational algorithms in predicting cancer driver mutations

  2020cited by this paper
• Analyses of non-coding somatic drivers in 2,658 cancer whole genomes

  2020cited by this paper
• High-throughput analysis of the activities of xCas9, SpCas9-NG and SpCas9 at matched and mismatched target sequences in human cells

  2020cited by this paper
• Guide RNAs with embedded barcodes boost CRISPR-pooled screens

  2019cited by this paper
• SpCas9 activity prediction by DeepSpCas9, a deep learning–based model with high generalization performance

  2019cited by this paper
• ClinVar: improvements to accessing data

  2019cited by this paper
• Prioritization of cancer therapeutic targets using CRISPR–Cas9 screens

  2019cited by this paper
• Next-generation characterization of the Cancer Cell Line Encyclopedia

  2019cited by this paper
• A Combined Approach Reveals a Regulatory Mechanism Coupling Src's Kinase Activity, Localization, and Phosphotransferase-Independent Functions.

  2019cited by this paper
• CRISPResso2 provides accurate and rapid genome editing sequence analysis

  2019cited by this paper
• Off-target RNA mutation induced by DNA base editing and its elimination by mutagenesis

  2019cited by this paper
• Continuous evolution of base editors with expanded target compatibility and improved activity

  2019cited by this paper
• Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction

  2018cited by this paper
• A Systematic p53 Mutation Library Links Differential Functional Impact to Cancer Mutation Pattern and Evolutionary Conservation.

  2018cited by this paper
• Crebbp Loss Drives Small Cell Lung Cancer and Increases Sensitivity to HDAC Inhibition.

  2018cited by this paper
• PHLDA1, another PHLDA family protein that inhibits Akt

  2018cited by this paper
• Mutational processes shape the landscape of TP53 mutations in human cancer

  2018cited by this paper
• The COSMIC Cancer Gene Census: describing genetic dysfunction across all human cancers

  2018cited by this paper
• Mfd Dynamically Regulates Transcription via a Release and Catch-Up Mechanism.

  2018cited by this paper
• Comprehensive Characterization of Cancer Driver Genes and Mutations

  2018cited by this paper
• Combinatorial CRISPR-Cas9 Metabolic Screens Reveal Critical Redox Control Points Dependent on the KEAP1-NRF2 Regulatory Axis.

  2018cited by this paper
• Evolved Cas9 variants with broad PAM compatibility and high DNA specificity

  2018cited by this paper
• Base editing with a Cpf1–cytidine deaminase fusion

  2018cited by this paper
• Engineered CRISPR-Cas9 nuclease with expanded targeting space

  2018cited by this paper
• Multiplex Assessment of Protein Variant Abundance by Massively Parallel Sequencing

  2018cited by this paper
• On the design of CRISPR-based single cell molecular screens

  2018cited by this paper
• Deep learning improves prediction of CRISPR–Cpf1 guide RNA activity

  2018cited by this paper
• A Multiplex Homology-Directed DNA Repair Assay Reveals the Impact of More Than 1,000 BRCA1 Missense Substitution Variants on Protein Function.

  2018cited by this paper
• Accurate classification of BRCA1 variants with saturation genome editing

  2018cited by this paper
• GUIDES: sgRNA design for loss-of-function screens

  2017cited by this paper
• Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions

  2017cited by this paper
• CRISPR-UMI: single-cell lineage tracing of pooled CRISPR–Cas9 screens

  2017cited by this paper
• Universal Patterns of Selection in Cancer and Somatic Tissues

  2017cited by this paper
• A communal catalogue reveals Earth’s multiscale microbial diversity

  2017cited by this paper
• CRISPR-STOP: gene silencing through base-editing-induced nonsense mutations

  2017cited by this paper
• CRISPR/Cas9 screening using unique molecular identifiers

  2017cited by this paper
• CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons.

  2017cited by this paper
• Codon optimality, bias and usage in translation and mRNA decay

  2017cited by this paper
• Structural Plasticity of PAM Recognition by Engineered Variants of the RNA-Guided Endonuclease Cas9.

  2016cited by this paper
• Pleckstrin homology-like domain, family A, member 1 (PHLDA1) and cancer.

  2016cited by this paper
• Phenotypic Characterization of a Comprehensive Set of MAPK1/ERK2 Missense Mutants.

  2016cited by this paper
• An extended set of yeast-based functional assays accurately identifies human disease mutations

  2016cited by this paper
• Epidermal Growth Factor Receptor P753S Mutation in Cutaneous Squamous Cell Carcinoma Responsive to Cetuximab-Based Therapy.

  2016cited by this paper
• Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems

  2016cited by this paper
• UMI-tools: Modelling sequencing errors in Unique Molecular Identifiers to improve quantification accuracy

  2016cited by this paper
• Prospective functional classification of all possible missense variants in PPARG

  2016cited by this paper
• Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

  2016cited by this paper
• In vivo high-throughput profiling of CRISPR–Cpf1 activity

  2016cited by this paper
• Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency

  2015cited by this paper
• Engineered CRISPR-Cas9 nucleases with altered PAM specificities

  2015cited by this paper
• Comparison of predicted and actual consequences of missense mutations

  2015cited by this paper
• Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.

  2015cited by this paper
• In vivo genome editing using Staphylococcus aureus Cas9

  2015cited by this paper
• Mechanism of initiation of aggregation of p53 revealed by Φ-value analysis

  2015cited by this paper
• Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

  2015cited by this paper
• Measuring error rates in genomic perturbation screens: gold standards for human functional genomics

  2014cited by this paper
• Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

  2014cited by this paper
• MAGeCK enables robust identification of essential genes from genome-scale CRISPR/Cas9 knockout screens

  2014cited by this paper
• A guide to genome engineering with programmable nucleases

  2014cited by this paper
• Improved vectors and genome-wide libraries for CRISPR screening

  2014cited by this paper
• Mutational landscape and significance across 12 major cancer types

  2013cited by this paper
• Systematic identification of molecular subtype-selective vulnerabilities in non-small-cell lung cancer.

  2013cited by this paper
• Caspase-8 as a therapeutic target in cancer.

  2013cited by this paper
• The hallmarks of cancer

  2012cited by this paper
• Creating Higher Titer Lentivirus with Caffeine

  2011cited by this paper
• Monozygotic twins with variable expression of Van der Woude Syndrome

  2011cited by this paper
• Hallmarks of cancer: the next generation.

  2011cited by this paper
• A competitive cell growth assay for the detection of subtle effects of gene transduction on cell proliferation

  2011cited by this paper
• Genotypic and Histological Evolution of Lung Cancers Acquiring Resistance to EGFR Inhibitors

  2011cited by this paper
• Developmental factor IRF6 exhibits tumor suppressor activity in squamous cell carcinomas

  2011cited by this paper
• International network of cancer genome projects

  2010cited by this paper
• Inactivating mutations of acetyltransferase genes in B-cell lymphoma

  2010cited by this paper
• A method and server for predicting damaging missense mutations

  2010cited by this paper
• NetPath: a public resource of curated signal transduction pathways

  2010cited by this paper
• Cancer-specific high-throughput annotation of somatic mutations: computational prediction of driver missense mutations.

  2009cited by this paper
• The cancer genome

  2009cited by this paper
• Comprehensive genomic characterization defines human glioblastoma genes and core pathways

  2008cited by this paper
• Combined targeting of the estrogen receptor and the epidermal growth factor receptor in non-small cell lung cancer shows enhanced antiproliferative effects.

  2005cited by this paper
• Comparison of single regulated lentiviral vectors with rtTA expression driven by an autoregulatory loop or a constitutive promoter

  2005cited by this paper
• The COSMIC (Catalogue of Somatic Mutations in Cancer) database and website

  2004cited by this paper
• Immortalization of Human Bronchial Epithelial Cells in the Absence of Viral Oncoproteins

  2004cited by this paper
• Predicting deleterious amino acid substitutions.

  2001cited by this paper

• Saturation genome editing-based clinical classification of BRCA2 variants

  2025cites this paper
• Research progress of base editing and prime editing tools based on the CRISPR/Cas system

  2025cites this paper
• A functional map of CDK-drug interactions at single amino acid resolution

  2025cites this paper
• High-throughput evaluation of in vitro CRISPR activities enables optimized large-scale multiplex enrichment of rare variants.

  2025cites this paper
• Comprehensive resistance profiling of chronic myeloid leukaemia associated ABL1 variants against five tyrosine kinase inhibitors using prime editing.

  2025cites this paper
• Fueling chromosomal gene diversification and artificial evolution with CRISPR

  2025cites this paper
• CRISPR libraries: A powerful screening tool for function and mechanism study of cancer

  2025cites this paper
• Evaluation and prediction of guide RNA activities in genome-editing tools

  2025influential citation
• Genetic mechanisms of resistance to targeted KRAS inhibition

  2025cites this paper
• Emerging trends in prime editing for precision genome editing

  2025cites this paper
• Nanopore-Based Strategy for Single-Nucleotide Mutation Detection in Sickle Cell Disease Using Anthracycline Probes.

  2025cites this paper
• Sensitive Transcriptomics and Genotyping reveals function of genetic variants in immunity

  2025cites this paper
• Multiplexed in vivo base editing identifies functional gene-variant-context interactions

  2025cites this paper
• Analysis of 10,478 cancer genomes identifies candidate driver genes and opportunities for precision oncology

  2024cites this paper
• Precise genome-editing in human diseases: mechanisms, strategies and applications

  2024cites this paper
• The herpesvirus UL49.5 protein hijacks a cellular C-degron pathway to drive TAP transporter degradation

  2024cites this paper
• High-throughput evaluation of genetic variants with prime editing sensor libraries

  2024cites this paper
• Recent advances in CRISPR-based functional genomics for the study of disease-associated genetic variants

  2024influential citation
• Deconstructing cancer with precision genome editing

  2024cites this paper
• Joint genotypic and phenotypic outcome modeling improves base editing variant effect quantification

  2024influential citation
• Analyzing the functional effects of DNA variants with gene editing

  2024cites this paper
• Accelerated drug-resistant variant discovery with an enhanced, scalable mutagenic base editor platform.

  2024cites this paper
• Harnessing the evolving CRISPR/Cas9 for precision oncology

  2024cites this paper
• The roles of patient‐derived xenograft models and artificial intelligence toward precision medicine

  2024cites this paper
• Base editing screens define the genetic landscape of cancer drug resistance mechanisms

  2024cites this paper
• Protocol for high-throughput screening of functional lysine residues in cell fitness

  2024cites this paper
• Saturation profiling of drug-resistant genetic variants using prime editing

  2024influential citation
• Precision mutational scanning: your multipass to the future of genetics

  2024cites this paper
• A comprehensive benchmark for multiple highly efficient base editors with broad targeting scope

  2024cites this paper
• Cancer driver mutations: predictions and reality.

  2023cites this paper
• Base editing screens map mutations affecting interferon-γ signaling in cancer

  2023cites this paper
• The herpesvirus UL49.5 protein hijacks a cellular C-degron pathway to drive TAP transporter degradation

  2023cites this paper
• Applications of CRISPR-Cas9 for advancing precision medicine in oncology: from target discovery to disease modeling

  2023cites this paper
• Unbiased interrogation of functional lysine residues in human proteome.

  2023cites this paper
• High throughput evaluation of genetic variants with prime editing sensor libraries

  2023cites this paper
• Revolutionizing Genetic Disease Treatment:Recent Technological Advances in Base Editing

  2023cites this paper
• Scanning mutagenesis of the voltage-gated sodium channel NaV1.2 using base editing

  2023cites this paper
• Revolutionizing DNA repair research and cancer therapy with CRISPR–Cas screens

  2023cites this paper
• High-throughput library transgenesis in Caenorhabditis elegans via Transgenic Arrays Resulting in Diversity of Integrated Sequences (TARDIS)

  2023cites this paper
• High-Throughput Identification, Modeling, and Analysis of Cancer Driver Genes In Vivo.

  2023cites this paper
• Base editor screens for in situ mutational scanning at scale.

  2023cites this paper
• Predicting and Improving Insertions by Prime Editing

  2023cites this paper
• How CRISPR Is Revolutionizing the Generation of New Models for Cancer Research.

  2023cites this paper
• Generation of precision preclinical cancer models using regulated in vivo base editing

  2023cites this paper
• Joint genotypic and phenotypic outcome modeling improves base editing variant effect quantification

  2023cites this paper
• Direct measurement of engineered cancer mutations and their transcriptional phenotypes in single cells

  2023cites this paper
• CRISPR‐Suppressor Scanning for Systematic Discovery of Drug‐Resistance Mutations

  2022cites this paper
• Base editor scanning charts the DNMT3A activity landscape

  2022cites this paper
• Assigning functionality to cysteines by base editing of cancer dependency genes

  2022cites this paper
• Genome editing and bioinformatics

  2022cites this paper
• Prime editing for precise and highly versatile genome manipulation

  2022cites this paper
• Single cell CRISPR base editor engineering and transcriptional characterization of cancer mutations

  2022cites this paper
• Pathogenic or benign?

  2022cites this paper