An autoactive NB-LRR gene causes Rht13 dwarfism in wheat

Semidwarfing genes have greatly increased wheat yields globally, yet the widely used gibberellin (GA) insensitive genes Rht-B1b and Rht-D1b have disadvantages for seedling emergence. Use of the GA sensitive semidwarfing gene Rht13 avoids this pleiotropic effect. Here we show that Rht13 encodes a nucleotide-binding site/leucine-rich repeat (NB-LRR) gene. A point mutation in the semidwarf Rht-B13b allele autoactivates the NB-LRR gene and causes a height reduction comparable to Rht-B1b and Rht-D1b in diverse genetic backgrounds. The autoactive Rht-B13b allele leads to transcriptional upregulation of pathogenesis-related genes including class III peroxidases associated with cell wall remodelling. Rht13 represents a new class of reduced height (Rht) gene, unlike other Rht genes which encode components of the GA signalling or metabolic pathways. This discovery opens new avenues to use autoactive NB-LRR genes as semidwarfing genes in a range of crop species, and to apply Rht13 in wheat breeding programmes using a perfect genetic marker.

• Publication year

  2022

• Venue

  bioRxiv

• Publication date

  2022-05-29

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1101/2022.05.28.493833PMID 36417432PMCID 9860330
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Rht24b, an ancient variation of TaGA2ox-A9, reduces plant height without yield penalty in wheat.

  2021influential reference
• Chromosome-scale genome assembly of the transformation-amenable common wheat cultivar ‘Fielder’

  2021cited by this paper
• Overgrowth mutants determine the causal role of GA2oxidaseA13 in Rht12 dwarfism of wheat.

  2020cited by this paper
• A haplotype-led approach to increase the precision of wheat breeding

  2020cited by this paper
• Limited haplotype diversity underlies polygenic trait architecture across 70 years of wheat breeding

  2020cited by this paper
• Multiple wheat genomes reveal global variation in modern breeding

  2020cited by this paper
• Effect of Gibberellin Responsive Reduced Height Allele Rht13 on Agronomic Traits in Spring Bread Wheat in Field Experiment in Non-Black Soil Zone

  2020cited by this paper
• A wheat dominant dwarfing line with Rht12, which reduces stem cell length and affects gibberellic acid synthesis, is a 5AL terminal deletion line

  2019cited by this paper
• RedoxiBase: A database for ROS homeostasis regulated proteins

  2019cited by this paper
• Genome-wide and evolutionary analysis of the class III peroxidase gene family in wheat and Aegilops tauschii reveals that some members are involved in stress responses

  2019cited by this paper
• Graph-based genome alignment and genotyping with HISAT2 and HISAT-genotype

  2019influential reference
• Hydrogen peroxide metabolism and functions in plants.

  2018influential reference
• Rht18 Semidwarfism in Wheat Is Due to Increased GA 2-oxidaseA9 Expression and Reduced GA Content1[OPEN]

  2018influential reference
• Shifting the limits in wheat research and breeding using a fully annotated reference genome

  2018cited by this paper
• Adaptive selection of founder segments and epistatic control of plant height in the MAGIC winter wheat population WM-800

  2018cited by this paper
• Adaptive selection of founder segments and epistatic control of plant height in the MAGIC winter wheat population WM-800

  2018cited by this paper
• Uncovering hidden variation in polyploid wheat

  2017influential reference
• Identification and characterization of Sr13, a tetraploid wheat gene that confers resistance to the Ug99 stem rust race group

  2017influential reference
• Repeat-length variation in a wheat cellulose synthase-like gene is associated with altered tiller number and stem cell wall composition

  2017cited by this paper
• Novel Rht-1 dwarfing genes: tools for wheat breeding and dissecting the function of DELLA proteins

  2017cited by this paper
• CDD/SPARCLE: functional classification of proteins via subfamily domain architectures

  2016cited by this paper
• Role of Reactive Oxygen Species during Cell Expansion in Leaves1[OPEN]

  2016cited by this paper
• Dissecting the U, M, S and C genomes of wild relatives of bread wheat (Aegilops spp.) into chromosomes and exploring their synteny with wheat.

  2016cited by this paper
• Tomato I2 immune receptor can be engineered to confer partial resistance to the oomycete Phytophthora infestans in addition to the fungus Fusarium oxysporum

  2015cited by this paper
• The wheat Sr50 gene reveals rich diversity at a cereal disease resistance locus

  2015cited by this paper
• PolyMarker: A fast polyploid primer design pipeline

  2015cited by this paper
• A recently evolved hexose transporter variant confers resistance to multiple pathogens in wheat

  2015cited by this paper
• Comparing the effects of GA-responsive dwarfing genes Rht13 and Rht8 on plant height and some agronomic traits in common wheat

  2015cited by this paper
• RNA-Seq bulked segregant analysis enables the identification of high-resolution genetic markers for breeding in hexaploid wheat.

  2015cited by this paper
• Heterologous expression and transcript analysis of gibberellin biosynthetic genes of grasses reveals novel functionality in the GA3ox family

  2015cited by this paper
• StringTie enables improved reconstruction of a transcriptome from RNA-seq reads

  2015influential reference
• Genetic effect of dwarfing gene Rht13 compared with Rht-D1b on plant height and some agronomic traits in common wheat (Triticum aestivum L.)

  2014cited by this paper
• Characterization of polyploid wheat genomic diversity using a high-density 90 000 single nucleotide polymorphism array

  2014cited by this paper
• Trimmomatic: a flexible trimmer for Illumina sequence data

  2014cited by this paper
• Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2

  2014influential reference
• The powdery mildew resistance gene Pm8 derived from rye is suppressed by its wheat ortholog Pm3.

  2014cited by this paper
• Efficient Agrobacterium transformation of elite wheat germplasm without selection

  2014cited by this paper
• Histochemical staining of Arabidopsis thaliana secondary cell wall elements.

  2014cited by this paper
• Genome-wide comparative diversity uncovers multiple targets of selection for improvement in hexaploid wheat landraces and cultivars

  2013cited by this paper
• Cell surface and cell outline imaging in plant tissues using the backscattered electron detector in a variable pressure scanning electron microscope

  2013cited by this paper
• Genetic Requirements for Signaling from an Autoactive Plant NB-LRR Intracellular Innate Immune Receptor

  2013cited by this paper
• A multiparent advanced generation inter-cross population for genetic analysis in wheat.

  2012cited by this paper
• Haplotype-based variant detection from short-read sequencing

  2012influential reference
• Height reduction and agronomic performance for selected gibberellin-responsive dwarfing genes in bread wheat (Triticum aestivum L.)

  2012influential reference
• REVIGO Summarizes and Visualizes Long Lists of Gene Ontology Terms

  2011influential reference
• Genetic mapping of gibberellic acid-sensitive genes for semi-dwarfism in durum wheat

  2011cited by this paper
• The Rht13 dwarfing gene reduces peduncle length and plant height to increase grain number and yield

  2011influential reference
• Identification of a Maize Locus That Modulates the Hypersensitive Defense Response, Using Mutant-Assisted Gene Identification and Characterization

  2010cited by this paper
• Breeding for improved water productivity in temperate cereals: phenotyping, quantitative trait loci, markers and the selection environment

  2010cited by this paper
• Semi-dwarfing Rht-B1 and Rht-D1 loci of wheat differ significantly in their influence on resistance to Fusarium head blight

  2009cited by this paper
• BLAST+: architecture and applications

  2009influential reference
• Open Access Method

  2009influential reference
• Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data

  2009cited by this paper
• The Sequence Alignment/Map format and SAMtools

  2009cited by this paper
• Coupling amplified DNA from flow-sorted chromosomes to high-density SNP mapping in barley

  2008influential reference
• Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation.

  2007cited by this paper
• A NAC Gene Regulating Senescence Improves Grain Protein, Zinc, and Iron Content in Wheat

  2006cited by this paper
• Mutations in the NB-ARC Domain of I-2 That Impair ATP Hydrolysis Cause Autoactivation1[OA]

  2006cited by this paper
• Artificial evolution extends the spectrum of viruses that are targeted by a disease-resistance gene from potato

  2006cited by this paper
• Chromosome Sorting in Tetraploid Wheat and Its Potential for Genome Analysis

  2005cited by this paper
• Autoactive alleles of the flax L6 rust resistance gene induce non-race-specific rust resistance associated with the hypersensitive response.

  2005cited by this paper
• Molecular mapping of gibberellin-responsive dwarfing genes in bread wheat

  2005influential reference
• A Haplotype-Specific Resistance Gene Regulated by BONZAI1 Mediates Temperature-Dependent Growth Control in Arabidopsis

  2004cited by this paper
• The effect of different height reducing genes on the early growth of wheat.

  2004cited by this paper
• Genome-Wide Analysis of NBS-LRR–Encoding Genes in Arabidopsis Online version contains Web-only data. Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.009308.

  2003cited by this paper
• The genes of the Green Revolution.

  2003cited by this paper
• A new mathematical model for relative quantification in real-time RT-PCR.

  2001cited by this paper
• ‘Green revolution’ genes encode mutant gibberellin response modulators

  1999influential reference
• Catalogue of gene symbols for wheat

  1998cited by this paper
• Hydrogen peroxide-mediated cell-wall stiffening in vitro in maize coleoptiles

  1996cited by this paper
• Influence of Semidwarfism and Genetic Background on Stand Establishment of Wheat1

  1980cited by this paper
• Rothamsted Repository Download

  year unknowncited by this paper

• Genetic identification and characterization of putatively novel, stable, and validated loci for plant height in wheat (Triticum aestivum L.).

  2026cites this paper
• Chromosome-Level Genome Assembly of Ormosia henryi Provides Insights into Evolutionary Resilience and Precision Conservation

  2026cites this paper
• Population transcriptome and phenotype analyses reveal that Rht-D1b contributed a larger seedling root to modern bread wheat.

  2025cites this paper
• Genetic dissection of plant height in spring wheat from Pakistan using genome-wide SNPs and allele-specific Rht markers

  2025cites this paper
• Loss of CsCLV2 function causes dwarfism and determinates growth in cucumber.

  2025cites this paper
• Genome-wide association study of agronomic traits in winter wheat (Triticum aestivum L.) using a custom SNP marker set

  2025influential citation
• Identification and development of KASP markers for genetic loci controlling plant height in bread wheat and evaluation their effects using near isogenic lines

  2025cites this paper
• Discovery of functional NLRs using expression level, high-throughput transformation and large-scale phenotyping

  2025cites this paper
• The allelic mutation of NBS-LRR gene causes premature senescence in wheat.

  2025cites this paper
• Identification of a novel dwarfing gene, Rht_m097, on chromosome 4BS in common wheat

  2025cites this paper
• Membrane-permeable trehalose 6-phosphate precursor spray increases wheat yields in field trials

  2025cites this paper
• A phosphatidylinositol 4-kinase alpha (PI4KA) gene reduces plant height of common wheat.

  2025cites this paper
• Genetic mapping and isolation of Rht28, a locus on wheat chromosome arm 2AL affecting plant height, grain size, and grain weight

  2025cites this paper
• Dynamic Transcriptome Analysis Uncovers Key Regulatory Genes During Stem Elongation Stages in Wheat

  2025cites this paper
• Molecular characteristics and regulatory effects of dwarfing Rht genes in Triticum aestivum L

  2025cites this paper
• Incorporating gene expression and environment for genomic prediction in wheat

  2024cites this paper
• Exploring Novel Genomic Loci and Candidate Genes Associated with Plant Height in Bulgarian Bread Wheat via Multi-Model GWAS

  2024cites this paper
• QTL analysis in the Mianmai 902×Taichang 29 RIL population reveals the genetic basis for the high-yield of wheat cultivars Mianmai 902 in terms of spike and plant architecture

  2024cites this paper
• Transcriptome and metabolome analyses reveal that GA3ox regulates the dwarf trait in mango (Mangifera indica L.)

  2024cites this paper
• A chromosome arm from Thinopyrum intermedium × Thinopyrum ponticum hybrid confers increased tillering and yield potential in wheat

  2024cites this paper
• Genetic analysis of late-maturity α-amylase in twelve wheat populations

  2024cites this paper
• Green Revolution dwarfing Rht genes negatively affected wheat floral traits related to cross-pollination efficiency.

  2024cites this paper
• Mapping and identification of a reverse mutation of Rht2 that enhances plant height and thousand grain weight in an elite wheat mutant induced by spaceflight.

  2024cites this paper
• Natural variant of Rht27, a dwarfing gene, enhances yield potential in wheat

  2024cites this paper
• The complex relationship between disease resistance and yield in crops

  2024cites this paper
• Cloning a novel reduced-height (Rht) gene TaOSCA1.4 from a QTL in wheat

  2024cites this paper
• Identifying genomic regions associated with key agro‐morphological traits in soft red winter wheat using genome‐wide association study

  2024cites this paper
• Research on the Effects of the Relationship between Agronomic Traits and Dwarfing Genes on Yield in Colored Wheat

  2024cites this paper
• BnaC03.BIN2 regulates plant height by affecting the main inflorescence length and first effective branch height in Brassica napus L

  2024cites this paper
• A novel regulator of wheat tillering LT1 identified by using an upgraded BSA method, uni-BSA

  2024cites this paper
• Activation and Autoinhibition Mechanisms of NLR Immune Receptor Pi36 in Rice

  2024cites this paper
• Partial redundancy buffers deleterious effects of mutating DNA methyltransferase 1-1 (MET1-1) in polyploid wheat

  2024influential citation
• Genetic regulation of wheat plant architecture and future prospects for its improvement

  2024cites this paper
• Genetic analysis and mapping of dwarf gene without yield penalty in a γ-ray-induced wheat mutant

  2023cites this paper
• Flow cytometric analysis and sorting of plant chromosomes

  2023cites this paper
• Rht12b, a widely used ancient allele of TaGA2oxA13, reduces plant height and enhances yield potential in wheat

  2023cites this paper
• Wheat plant height locus RHT25 encodes a PLATZ transcription factor that interacts with DELLA (RHT1)

  2023cites this paper
• Identification of a novel SNP in the miR172 binding site of Q homoeolog AP2L-D5 is associated with spike compactness and agronomic traits in wheat (Triticum aestivum L.)

  2023cites this paper
• Integration of genome-wide association study, linkage analysis, and population transcriptome analysis to reveal the TaFMO1-5B modulating seminal root growth in bread wheat.

  2023cites this paper
• In silico curation of QTL-rich clusters and candidate gene identification for plant height of bread wheat

  2023cites this paper
• Fine mapping and characterization of a major QTL for plant height on chromosome 5A in wheat

  2023cites this paper
• Genetic mapping and identification of Rht8-B1 that regulates plant height in wheat

  2023cites this paper
• Genetic mapping and identi�cation of Rht8-B1 that regulates plant height in wheat

  year unknowncites this paper