Versatile Transformation System That Is Applicable to both Multiple Transgene Expression and Gene Targeting for Thraustochytrids

No abstract is available for this paper.

• Publication year

  2012

• Venue

  Applied and Environmental Microbiology

• Publication date

  2012-02-17

• Fields of study

  Biology, Medicine, Engineering

• Identifiers
  DOI 10.1128/AEM.07129-11PMID 22344656PMCID PMC3346472
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Increase of Eicosapentaenoic Acid in Thraustochytrids through Thraustochytrid Ubiquitin Promoter-Driven Expression of a Fatty Acid Δ5 Desaturase Gene

  2011cited by this paper
• Catalytic cracking of squalene to gasoline-range molecules

  2011cited by this paper
• Establishment of a transgene expression system for the marine microalga Schizochytrium by 18S rDNA-targeted homologous recombination

  2011influential reference
• Molecular cloning of a Pinguiochrysis pyriformis oleate-specific microsomal Δ12-fatty acid desaturase and functional analysis in yeasts and thraustochytrids.

  2011cited by this paper
• Thraustochytrid Aurantiochytrium sp. 18W-13a Accummulates High Amounts of Squalene

  2011cited by this paper
• Cardiovascular effects of marine omega-3 fatty acids.

  2010cited by this paper
• Head of German cost-effectiveness institute forced to quit.

  2010cited by this paper
• Improved production of various polyunsaturated fatty acids through filamentous fungus Mortierella alpina breeding

  2009cited by this paper
• Characterization of Both Polyunsaturated Fatty Acid Biosynthetic Pathways in Schizochytrium sp.

  2009influential reference
• Biochemical characterization of polyunsaturated fatty acid synthesis in Schizochytrium: release of the products as free fatty acids.

  2009influential reference
• Optimization of culture conditions for growth and docosahexaenoic acid production by a marine thraustochytrid, Aurantiochytrium limacinum mh0186.

  2009influential reference
• Taxonomic rearrangement of the genus Schizochytrium sensu lato based on morphology, chemotaxonomic characteristics, and 18S rRNA gene phylogeny (Thraustochytriaceae, Labyrinthulomycetes): emendation for Schizochytrium and erection of Aurantiochytrium and Oblongichytrium gen. nov.

  2007influential reference
• Taxonomic rearrangement of the genus Ulkenia sensu lato based on morphology, chemotaxonomical characteristics, and 18S rRNA gene phylogeny (Thraustochytriaceae, Labyrinthulomycetes): emendation for Ulkenia and erection of Botryochytrium, Parietichytrium, and Sicyoidochytrium gen. nov.

  2007influential reference
• Folate and long-chain polyunsaturated fatty acids in psychiatric disease.

  2006cited by this paper
• Generation of cloned transgenic pigs rich in omega-3 fatty acids

  2006cited by this paper
• Stepwise engineering to produce high yields of very long-chain polyunsaturated fatty acids in plants

  2005cited by this paper
• Fatty acids from lipids of marine organisms: molecular biodiversity, roles as biomarkers, biologically active compounds, and economical aspects.

  2005cited by this paper
• The New Higher Level Classification of Eukaryotes with Emphasis on the Taxonomy of Protists

  2005cited by this paper
• Metabolic engineering of Arabidopsis to produce nutritionally important DHA in seed oil.

  2005cited by this paper
• Biosynthesis of Very-Long-Chain Polyunsaturated Fatty Acids in Transgenic Oilseeds: Constraints on Their Accumulationw⃞

  2004cited by this paper
• Functional expression of a Delta12 fatty acid desaturase gene from spinach in transgenic pigs.

  2004cited by this paper
• Transgenic mice: Fat-1 mice convert n-6 to n-3 fatty acids

  2004cited by this paper
• Lipid class composition of the microalga Pavlova lutheri: eicosapentaenoic and docosahexaenoic acids.

  2003cited by this paper
• A Brevibacterium lactofermentum 16S rRNA gene used as target site for homologous recombination.

  2000cited by this paper
• Isolation and Use of a Homologous Histone H4 Promoter and a Ribosomal DNA Region in a Transformation Vector for the Oil-Producing Fungus Mortierella alpina

  2000cited by this paper
• Long-chain n−3 polyunsaturated fatty acid production by members of the marine protistan group the thraustochytrids: screening of isolates and optimisation of docosahexaenoic acid production

  1999cited by this paper
• The Biotechnological Potential of Thraustochytrids

  1999cited by this paper
• High-copy-number integration into the ribosomal DNA of Saccharomyces cerevisiae: a new vector for high-level expression.

  1989cited by this paper

• Characterization and Application of Schizochytrium sp. HX-308 Promoters for Enhanced Docosahexaenoic Acid Biosynthesis

  2025cites this paper
• Novel centromeric plasmid for stable extrachromosomal gene expression in Aurantiochytrium limacinum

  2025cites this paper
• Lipid Droplet Degradation Through Lipophagy in Aurantiochytrium limacinum mh0186

  2025cites this paper
• ACP Activation Enhances Differential Fatty Acid Accumulation in Engineered Thraustochytrids via Optimized Transformation.

  2025cites this paper
• Transgene-free protein-based genome editing in thraustochytrids enables customizable modulation of long-chain polyunsaturated fatty acid profiles

  2025cites this paper
• Enhancing fatty acid and omega-3 production in Schizochytrium sp. using developed safe-harboring expression system

  2024cites this paper
• Heterologous expression of phytase in Schizochytrium sp. as a fortified feed additive for the Livestock industry

  2023cites this paper
• Characterization of thraustochytrid-specific sterol O-acyltransferase: modification of DGAT2-like enzyme to increase the sterol production in Aurantiochytrium limacinum mh0186

  2023cites this paper
• Natural and recombinant bioactive compounds from Schizochytrium sp.: Recent advances and future prospects

  2023cites this paper
• Enhancement of Squalene Production by Constitutive Expression of the 3-Hydroxy-3-Methylglutaryl-CoA Reductase in Aurantiochytrium sp. 18W-13a

  2022cites this paper
• Modulating DHA-Producing Schizochytrium sp. toward Astaxanthin Biosynthesis via a Seamless Genome Editing System.

  2022cites this paper
• Deciphering and engineering the polyunsaturated fatty acid synthase pathway from eukaryotic microorganisms

  2022cites this paper
• Improvement of Astaxanthin Production in Aurantiochytrium limacinum by Overexpression of the Beta-Carotene Hydroxylase Gene

  2022cites this paper
• Proteomic and lipidomic analyses of lipid droplets in Aurantiochytrium limacinum ATCC MYA-1381

  2022cites this paper
• Significance of mitochondrial fatty acid β-oxidation for the survivability of Aurantiochytrium limacinum ATCC MYA-1381 during sugar starvation.

  2022cites this paper
• Strategic Development of Aurantiochytrium sp. Mutants With Superior Oxidative Stress Tolerance and Glucose-6-Phosphate Dehydrogenase Activity for Enhanced DHA Production Through Plasma Mutagenesis Coupled With Chemical Screening

  2022cites this paper
• Microbial genetic engineering approach to replace shark livering for squalene.

  2022cites this paper
• Recent trends in the field of lipid engineering.

  2022cites this paper
• Biotechnological production of lipid and terpenoid from thraustochytrids.

  2021cites this paper
• Development of an Efficient Gene Editing Tool in Schizochytrium sp. and Improving Its Lipid and Terpenoid Biosynthesis

  2021cites this paper
• PUFA synthase-independent DHA synthesis pathway in Parietichytrium sp. and its modification to produce EPA and n-3DPA

  2021cites this paper
• Method Development Progress in Genetic Engineering of Thraustochytrids

  2021influential citation
• A non-canonical Δ9-desaturase synthesizing palmitoleic acid identified in the thraustochytrid Aurantiochytrium sp. T66

  2021cites this paper
• C4-monomethylsterol β-glucoside and its synthase in Aurantiochytrium limacinum mh0186.

  2021cites this paper
• Combined Metabolome and Lipidome Analyses for In-Depth Characterization of Lipid Accumulation in the DHA Producing Aurantiochytrium sp. T66

  2021cites this paper
• Enhanced Production of Astaxanthin without Decrease of DHA Content in Aurantiochytrium limacinum by Overexpressing Multifunctional Carotenoid Synthase Gene

  2020cites this paper
• Enhancement of docosahexaenoic acid production by overexpression of ATP-citrate lyase and acetyl-CoA carboxylase in Schizochytrium sp.

  2020cites this paper
• Genome and Transcriptome Analyses Provide Insight Into the Omega-3 Long-Chain Polyunsaturated Fatty Acids Biosynthesis of Schizochytrium limacinum SR21

  2020cites this paper
• The strategies to reduce cost and improve productivity in DHA production by Aurantiochytrium sp.: from biochemical to genetic respects

  2020cites this paper
• Improvement of fatty acid productivity of thraustochytrid, Aurantiochytrium sp. by genome editing.

  2020cites this paper
• Genetic tool development in marine protists: emerging model organisms for experimental cell biology

  2019cites this paper
• The glycerol-3-phosphate acyltransferase PLAT2 functions in the generation of DHA-rich glycerolipids in Aurantiochytrium limacinum F26-b

  2019cites this paper
• Role of flow cytometry for the improvement of bioprocessing of oleaginous microorganisms

  2019cites this paper
• Genetic modification of the thraustochytrid Aurantiochytrium sp. 18W-13a for cellobiose utilization by secretory expression of β-glucosidase from Aspergillus aculeatus

  2019cites this paper
• The lipid metabolism in THRAUSTOCHYTRIDS.

  2019influential citation
• A thraustochytrid-specific lipase/phospholipase with unique positional specificity contributes to microbial competition and fatty acid acquisition from the environment

  2019cites this paper
• Publisher Correction: Genetic tool development in marine protists: emerging model organisms for experimental cell biology

  2019cites this paper
• First International Conference on Labyrinthulean Protists (ICoLP)

  2019cites this paper
• Visualization of Endoplasmic Reticulum and Mitochondria in Aurantiochytrium limacinum by the Expression of EGFP with Cell Organelle-Specific Targeting/Retaining Signals

  2018cites this paper
• Overexpression of Malonyl-CoA: ACP Transacylase in Schizochytrium sp. to Improve Polyunsaturated Fatty Acid Production.

  2018cites this paper
• Cloning of the pks3 gene of Aurantiochytrium limacinum and functional study of the 3-ketoacyl-ACP reductase and dehydratase enzyme domains

  2018cites this paper
• Prospects on the Use of Schizochytrium sp. to Develop Oral Vaccines

  2018cites this paper
• Engineering xylose metabolism in thraustochytrid T18

  2018cites this paper
• Functions of PKS Genes in Lipid Synthesis of Schizochytrium sp. by Gene Disruption and Metabolomics Analysis

  2018cites this paper
• Characterization and Application of Marine Microbial Omega-3 Polyunsaturated Fatty Acid Synthesis.

  2018cites this paper
• Alleviation of reactive oxygen species enhances PUFA accumulation in Schizochytrium sp. through regulating genes involved in lipid metabolism

  2018cites this paper
• Mining terpenoids production and biosynthetic pathway in thraustochytrids.

  2017cites this paper
• Regulation of TG accumulation and lipid droplet morphology by the novel TLDP1 in Aurantiochytrium limacinum F26-b[S]

  2017cites this paper
• Physiology, Biochemistry, and Biotechnology

  2017cites this paper
• Transcriptomic Analysis of the Regulation of Lipid Fraction Migration and Fatty Acid Biosynthesis in Schizochytrium sp.

  2017cites this paper
• Taxonomy, ecology and biotechnological applications of thraustochytrids: A review.

  2017cites this paper
• Glass Bead-based Genetic Transformation:An Efficient Method for Transformation of Thraustochytrid Microorganisms.

  2017cites this paper
• Overexpression of glucose-6-phosphate dehydrogenase enhanced the polyunsaturated fatty acid composition of Aurantiochytrium sp. SD116

  2016cites this paper
• Thraustochytrids as production organisms for docosahexaenoic acid (DHA), squalene, and carotenoids

  2016cites this paper
• Improved docosahexaenoic acid production in Aurantiochytrium by glucose limited pH-auxostat fed-batch cultivation.

  2015cites this paper
• Diversity and Ecology of Thraustochytrid Protists in the Marine Environment

  2015cites this paper
• Mechanisms of fatty acid synthesis in marine fungus-like protists

  2015cites this paper
• Study of cellular development and intracellular lipid bodies accumulation in the thraustochytrid Aurantiochytrium sp. KRS101.

  2014cites this paper
• Recombinant Protein Production with Microalgae

  2014cites this paper
• Enhanced production of fatty acids and astaxanthin in Aurantiochytrium sp. by the expression of Vitreoscilla hemoglobin.

  2014cites this paper
• Novel Lysophospholipid Acyltransferase PLAT1 of Aurantiochytrium limacinum F26-b Responsible for Generation of Palmitate-Docosahexaenoate-Phosphatidylcholine and Phosphatidylethanolamine

  2014cites this paper
• Ecological dynamics and biotechnological implications of thraustochytrids from marine habitats

  2014cites this paper
• A transgene expression system for the marine microalgae Aurantiochytrium sp. KRS101 using a mutant allele of the gene encoding ribosomal protein L44 as a selectable transformation marker for cycloheximide resistance

  2013cites this paper
• Two Fatty Acid Elongases Possessing C18-Δ6/C18-Δ9/C20-Δ5 or C16-Δ9 Elongase Activity in Thraustochytrium sp. ATCC 26185

  2013cites this paper
• Genetic tool development in marine protists: emerging model Genetic tool development in marine protists: emerging model organisms for experimental cell biology organisms for experimental cell biology

  year unknowncites this paper