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Determination of the steady-state intracellular chloride concentration in capacitated human spermatozoa.

Manuel A. Garcia,Stanley Meizel

Published 1999 in Journal of Andrology

ABSTRACT

Chloride channels participate in the mammalian sperm acrosome reaction (AR). However, the mechanism by which sperm regulate intracellular Cl- concentration ([Cl-]i) is not known. The steady-state [Cl-]i has also never been reported for mammalian spermatozoa. Therefore, using chloride-sensitive fluorescent dyes, we sought to determine the steady-state [Cl-]i of capacitated human spermatozoa by a null-point measurement technique. We found that the [Cl-]i was sufficiently elevated (a conservative estimate of > or = 41 mM) such that the opening of chloride channels should result in a Cl- efflux and, hence, in depolarization. Moreover, the [Cl-]i does not remain constant under varying extracellular Cl- concentrations ([Cl-]o).

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