Acetylcholine-induced chloride current oscillations in swine tracheal smooth muscle cells.

The activation of muscarinic receptors by acetylcholine (ACh) in tracheal smooth muscle cells induced Ca++ oscillations, measured as the activation of Ca(++)-dependent Cl- currents (Clca) at the K+ equilibrium potential. The currents were not abolished by replacement of external and internal K+ with Cs+ but decreased after reduction in internal Cl- concentration or extracellular application of niflumic acid, a Cl- channel blocker. The Clca oscillations were dependent on external Ca++ concentration ([Ca++]e). The mean current and frequency increased with increasing [Ca++]e, were enhanced by Bay K 8644 and were inhibited by verapamil, suggesting a role for voltage-operated Ca++ channels (VOC). Steady-state increases in Clca induced by 10(-6) M ACh could be converted to oscillations by reducing [Ca++]e or by buffering intracellular Ca++ with ethyleneglycolbis-N,N,N'-N'-tetraacetic acid (EGTA). Oscillations in Clca induced by 3 x 10(-8) and 10(-7) M ACh were more sensitive to EGTA than those induced by 10(-6) M ACh. Caffeine induced nonoscillatory, transient increases in Clca and reduced subsequent ACh-induced increases in Clca. The oscillatory patterns of Clca induced by ACh and the effects of modification of Ca++ influx were similar for oscillations in intracellular Ca++ concentration [Ca++]i as measured with confocal microfluorimetry. Thus, ACh-induced Clca oscillations reflect fluctuations in [Ca++]i that are consistent with initiation of Ca++ release from inositol-1,4,5-trisphosphate-(IP3)sensitive Ca++ stores. Maintenance of the oscillations requires Ca++ influx, in part through voltage-operated Ca++ channels.

• Publication year

  1996

• Venue

  Journal of Pharmacology and Experimental Therapeutics

• Publication date

  1996-01-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0022-3565(25)12247-7PMID 8558428
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Superficial buffer barrier function of smooth muscle sarcoplasmic reticulum.

  1995cited by this paper
• Inositol 1,4,5-trisphosphate receptors in Xenopus laevis oocytes: localization and modulation by Ca2+.

  1994cited by this paper
• Caffeine induces periodic oscillations of Ca2+-activated K+ current in pulmonary arterial smooth muscle cells

  1994cited by this paper
• Effects of Cl channel blockers on Ca‐activated chloride and potassium currents in smooth muscle cells from rabbit portal vein

  1994cited by this paper
• Muscarinic-receptor activation stimulates oscillations in K+ and Cl− currents which are acutely dependent on extracellular Ca2+ in avian salt gland cells

  1994cited by this paper
• Oscillations of cytosolic calcium in rat chromaffin cells: dual modulation in frequency and amplitude.

  1994cited by this paper
• The biology and medicine of calcium signalling.

  1994cited by this paper
• Mechanisms and function of intercellular calcium signaling.

  1994cited by this paper
• Caffeine activates a Ca(2+)-permeable, nonselective cation channel in smooth muscle cells

  1994cited by this paper
• Signal transduction and regulation in smooth muscle

  1994cited by this paper
• A dialogue on Ca2+ oscillations: an attempt to understand the essentials of mechanisms leading to hormone-induced intracellular Ca2+ oscillations in various kinds of cell on a theoretical level.

  1994cited by this paper
• Subcellular Ca(2+)-gradients in A7r5 vascular smooth muscle.

  1994cited by this paper
• Receptor-activated Ca2+ influx: how many mechanisms for how many channels?

  1994cited by this paper
• Photoreleased inositol 1,4,5‐trisphosphate‐induced response in single smooth muscle cells of rat portal vein.

  1994cited by this paper
• Ca2+ inhibition of inositol trisphosphate‐induced Ca2+ release in single smooth muscle cells of guinea‐pig small intestine.

  1994cited by this paper
• Calcium-activated chloride current in rabbit coronary artery myocytes.

  1994cited by this paper
• Inward current activated by carbachol in rat intestinal smooth muscle cells.

  1993cited by this paper
• A model of calcium regulation in smooth muscle cell.

  1993cited by this paper
• Intracellular calcium waves generated by Ins(1,4,5)P3-dependent mechanisms.

  1993cited by this paper
• Effect of voltage-gated plasma membrane Ca2+ fluxes on IP3-linked Ca2+ oscillations.

  1993cited by this paper
• Inositol trisphosphate and calcium signalling

  1993cited by this paper
• Time course of spontaneous calcium‐activated chloride currents in smooth muscle cells from the rabbit portal vein.

  1993cited by this paper
• Kinetics of ATP‐induced Ca2+ transients in cultured pig aortic smooth muscle cells depend on ATP concentration and stored Ca2+.

  1993cited by this paper
• Chloride currents activated by caffeine in rat intestinal smooth muscle cells.

  1993cited by this paper
• A single-pool model for intracellular calcium oscillations and waves in the Xenopus laevis oocyte.

  1993cited by this paper
• Emptying and refilling of Ca2+ store in tracheal myocytes as indicated by ACh-evoked currents and contraction.

  1993cited by this paper
• Intracellular calcium ions activate a low-conductance chloride channel in smooth-muscle cells isolated from human mesenteric artery

  1993cited by this paper
• Oscillations of receptor-operated cationic current and internal calcium in single guinea-pig ileal smooth muscle cells

  1993cited by this paper
• Muscarinic regulation of cytosolic free calcium in canine tracheal smooth muscle cells: Ca2+ requirement for phospholipase C activation

  1993cited by this paper
• Verapamil and diltiazem inhibit receptor‐operated calcium channels and intracellular calcium oscillations in rat hepatocytes

  1993cited by this paper
• Muscarinic regulation of membrane ion channels in airway smooth muscle cells.

  1992cited by this paper
• Signal transduction in smooth muscle as studied by the subcellular membrane approach.

  1992cited by this paper
• Acetylcholine activates non‐selective cation and chloride conductances in canine and guinea‐pig tracheal myocytes.

  1992cited by this paper
• Inositol 1,4,5-trisphosphate compartmentalization in tracheal smooth muscle.

  1992cited by this paper
• Properties of spontaneous inward currents recorded in smooth muscle cells isolated from the rabbit portal vein.

  1992cited by this paper
• Characterization of large‐conductance chloride channels in rabbit colonic smooth muscle.

  1992cited by this paper
• Relation between muscarinic receptor cationic current and internal calcium in guinea‐pig jejunal smooth muscle cells.

  1991cited by this paper
• Inositol 1,4,5-trisphosphate- and guanosine 5'-O-(3-thiotriphosphate)-induced Ca2+ release in cultured airway smooth muscle.

  1991cited by this paper
• Ca2+ oscillations induced by histamine H1 receptor stimulation in HeLa cells: Fura-2 and patch clamp analysis.

  1991cited by this paper
• Oscillating intracellular Ca2+ signals evoked by activation of receptors linked to inositol lipid hydrolysis: Mechanism of generation

  1990cited by this paper
• Calcium channels, stores, and oscillations.

  1990cited by this paper
• Inositol phosphates and cell signalling

  1989cited by this paper
• Calcium oscillations in non-excitable cells.

  1989cited by this paper
• Mass changes of inositol(1,4,5)trisphosphate in trachealis muscle following agonist stimulation.

  1989cited by this paper
• Ca2+ oscillations induced by hormonal stimulation of individual fura-2-loaded hepatocytes.

  1989cited by this paper
• Repetitive spikes in cytoplasmic calcium evoked by histamine in human endothelial cells

  1988cited by this paper
• Agonist-induced oscillations in cytoplasmic free calcium concentration in single rat hepatocytes.

  1987cited by this paper
• Repetitive transient rises in cytoplasmic free calcium in hormone-stimulated hepatocytes

  1986cited by this paper
• Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches

  1981cited by this paper

• Mitochondrial regulation of airway smooth muscle functions in health and pulmonary diseases.

  2019influential citation
• Drug Repurposing: The Anthelmintics Niclosamide and Nitazoxanide Are Potent TMEM16A Antagonists That Fully Bronchodilate Airways

  2018cites this paper
• Assessment of the Airway Smooth Muscle Relaxant Effect of Drotaverine

  2018cites this paper
• smooth muscle cells on acetylcholine-induced membrane current in airway Selected Contribution: Effect of the aldehyde acrolein

  2015cites this paper
• airway smooth muscle Temporal aspects of excitation-contraction coupling in

  2015cites this paper
• Role of N-methyl-D-aspartate receptors in the regulation of human airway smooth muscle function and airway responsiveness

  2015cites this paper
• Calcium-Activated Chloride Channels

  2014influential citation
• Kv7 (KCNQ) Potassium Channels and L-type Calcium Channels in the Regulation of Airway Diameter

  2014cites this paper
• Temporal Aspects of Ca2+ Signaling in Airway Myocytes

  2014cites this paper
• Local Calcium Signaling in Airway Smooth Muscle Cells

  2014cites this paper
• Ryanodine and Inositol Trisphosphate Receptors/Ca2+ Release Channels in Airway Smooth Muscle Cells

  2014cites this paper
• Ion channel regulation of intracellular calcium and airway smooth muscle function.

  2009cites this paper
• Regulation of airway smooth muscle cell contractility by Ca2+ signaling and sensitivity.

  2008cites this paper
• Smooth muscle cell calcium activation mechanisms

  2008cites this paper
• Ionic mechanisms and Ca2+ handling in airway smooth muscle

  2007cites this paper
• Airway smooth muscle dynamics: a common pathway of airway obstruction in asthma.

  2007cites this paper
• The generation of acetylcholine-induced asynchronous Ca²⁺ waves and their role in airway smooth muscle

  2006cites this paper
• Involvement of ryanodine receptors in muscarinic receptor-mediated membrane current oscillation in urinary bladder smooth muscle.

  2005cites this paper
• Vasomotion: cellular background for the oscillator and for the synchronization of smooth muscle cells

  2005cites this paper
• Cellular signaling mechanisms for muscarinic acetylcholine receptors.

  2003cites this paper
• Cellular Signaling Mechanisms for Muscarinic Acetylcholine Receptors

  2003cites this paper
• Ionic mechanisms and Ca(2+) regulation in airway smooth muscle contraction: do the data contradict dogma?

  2002cites this paper
• The influence of different InsP(3) receptor isoforms on Ca(2+) signaling in tracheal smooth muscle cells.

  2002cites this paper
• Acetylcholine-induced Calcium Signaling and Contraction of Airway Smooth Muscle Cells in Lung Slices

  2002cites this paper
• exposure to gas pollutants on airway reactivity

  2002cites this paper
• The influence of different InsP3 receptor isoforms on Ca2+ signaling in tracheal smooth muscle cells

  2002cites this paper
• Combined effect of chronic hypoxia and in vitro exposure to gas pollutants on airway reactivity.

  2002cites this paper
• ATP stimulates Ca2+ oscillations and contraction in airway smooth muscle cells of mouse lung slices.

  2002cites this paper
• Selected contribution: effect of the aldehyde acrolein on acetylcholine-induced membrane current in airway smooth muscle cells.

  2001cites this paper
• Temporal aspects of excitation-contraction coupling in airway smooth muscle.

  2001cites this paper
• Signal Transduction in Smooth Muscle Invited Review : Significance of spatial and temporal heterogeneity of calcium transients in smooth muscle

  2001cites this paper
• Modelling of Ca2+-Activated Chloride Current in Tracheal Smooth Muscle Cells

  2001cites this paper
• Simultaneous recording of mechanical and intracellular electrical activity in human urinary bladder smooth muscle

  2000cites this paper
• Cellular mechanisms of acrolein-induced alteration in calcium signaling in airway smooth muscle.

  2000cites this paper
• Spatial and temporal aspects of ACh-induced [Ca2+]i oscillations in porcine tracheal smooth muscle.

  2000cites this paper
• The role of intracellular and extracellular calcium in mechanical and intracellular electrical activity of human urinary bladder smooth muscle

  2000cites this paper
• Spatial and temporal aspects of calcium sparks in porcine tracheal smooth muscle cells.

  1999cites this paper
• Effect of halothane on intracellular calcium oscillations in porcine tracheal smooth muscle cells.

  1999cites this paper
• Spatial and temporal aspects of calcium sparks in porcine tracheal smooth muscle cells.

  1999cites this paper
• Neuronal Ca 2 +-activated Cl ÿ channels Ð homing in on an elusive channel species

  1999cites this paper
• Effect of halothane on intracellular calcium oscillations in porcine tracheal smooth muscle cells.

  1999cites this paper
• Properties of a new calcium-permeable single channel from tracheal microsomes.

  1999cites this paper
• ALUNG November 21/5

  1999cites this paper
• Spatial and temporal aspects of calcium sparks in porcine tracheal smooth muscle cells PROPAGATING OSCILLATIONS

  1999cites this paper
• Physiological features of visceral smooth muscle cells, with special reference to receptors and ion channels.

  1998cites this paper
• Effects of 8-bromo-cyclic GMP on membrane potential of single swine tracheal smooth muscle cells.

  1998cites this paper
• Role of cyclic ADP-ribose in the regulation of [Ca2+]i in porcine tracheal smooth muscle.

  1998cites this paper
• Role of cyclic ADP-ribose in the regulation of [Ca2+]i in porcine tracheal smooth muscle.

  1998cites this paper
• Acetylcholine-induced membrane potential oscillations in the intact lens.

  1998cites this paper
• Calcium signaling in airway smooth muscle cells is altered by in vitro exposure to the aldehyde acrolein.

  1998cites this paper
• Effects of 8-Bromo-Cyclic GMP on Membrane Potential of Single Swine Tracheal Smooth Muscle Cells 1

  1998influential citation
• [Ca2+]i oscillations induced by muscarinic stimulation in airway smooth muscle cells: receptor subtypes and correlation with the mechanical activity

  1997cites this paper
• Nitric oxide inhibits ACh-induced intracellular calcium oscillations in porcine tracheal smooth muscle.

  1997cites this paper
• Heterogeneity in dynamic regulation of intracellular calcium in airway smooth muscle cells.

  1997cites this paper
• Role of ryanodine receptor channels in Ca2+ oscillations of porcine tracheal smooth muscle.

  1997cites this paper
• Effects of salbutamol on intracellular calcium oscillations in porcine airway smooth muscle.

  1997influential citation
• Regulation of intracellular calcium oscillations in porcine tracheal smooth muscle cells.

  1997cites this paper
• Cellular mechanisms underlying carbachol‐induced oscillations of calcium‐dependent membrane current in smooth muscle cells from mouse anococcygeus

  1997influential citation
• Frequency modulation of acetylcholine-induced oscillations in Ca++ and Ca(++)-activated Cl- current by cAMP in tracheal smooth muscle.

  1996cites this paper
• Acetylcholine-induced Ca++-dependent chloride current oscillations are mediated by inositol 1,4,5-trisphosphate in tracheal myocytes.

  1996cites this paper
• Intracellular calcium stores in isolated tracheal smooth muscle cells.

  1996cites this paper
• Depletion and refilling of acetylcholine- and caffeine-sensitive Ca++ stores in tracheal myocytes.

  1996cites this paper
• Inhibition by sodium nitroprusside of a calcium store depletion‐activated non‐selective cation current in smooth muscle cells of the mouse anococcygeus

  1996cites this paper
• Effects of Elevated Cytosolic Calcium on ACh-Induced Swine Tracheal Smooth Muscle Contraction.

  1996cites this paper