Small-molecule control of protein degradation using split adaptors.

No abstract is available for this paper.

• Publication year

  2011

• Venue

  ACS Chemical Biology

• Publication date

  2011-11-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1021/cb2001389PMID 21866931PMCID PMC3220803
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Design, construction and characterization of a set of insulated bacterial promoters

  2010cited by this paper
• Protein inactivation in mycobacteria by controlled proteolysis and its application to deplete the beta subunit of RNA polymerase

  2010cited by this paper
• Understanding and harnessing energy-dependent proteolysis for controlled protein degradation in bacteria

  2010cited by this paper
• Polypeptide translocation by the AAA+ ClpXP protease machine.

  2009cited by this paper
• Spatiotemporal Control of Cell Signalling Using A Light-Switchable Protein Interaction

  2009cited by this paper
• Two functions of the C-terminal domain of Escherichia coli Rob: mediating "sequestration-dispersal" as a novel off-on switch for regulating Rob's activity as a transcription activator and preventing degradation of Rob by Lon protease.

  2009cited by this paper
• Efficient protein depletion by genetically controlled deprotection of a dormant N-degron

  2009cited by this paper
• Single-molecule denaturation and degradation of proteins by the AAA+ ClpXP protease

  2009cited by this paper
• Crystal structures of asymmetric ClpX hexamers reveal nucleotide-dependent motions in a AAA+ protein-unfolding machine

  2009cited by this paper
• Engineering Synthetic Adaptors and Substrates for Controlled ClpXP Degradation*

  2009cited by this paper
• Inducible protein degradation in Bacillus subtilis using heterologous peptide tags and adaptor proteins to target substrates to the protease ClpXP

  2008influential reference
• Forced extraction of targeted components from complex macromolecular assemblies

  2008cited by this paper
• A fast, robust, and tunable synthetic gene oscillator

  2008cited by this paper
• Altered Tethering of the SspB Adaptor to the ClpXP Protease Causes Changes in Substrate Delivery*

  2007cited by this paper
• Scarless and site-directed mutagenesis in Salmonella enteritidis chromosome

  2007cited by this paper
• Chemical genetics: where genetics and pharmacology meet.

  2007cited by this paper
• Direct and adaptor-mediated substrate recognition by an essential AAA+ protease

  2007cited by this paper
• Engineering controllable protein degradation.

  2006cited by this paper
• A set of recombineering plasmids for gram-negative bacteria.

  2006cited by this paper
• A rapid, reversible, and tunable method to regulate protein function in living cells using synthetic small molecules.

  2006cited by this paper
• ATP-dependent proteases of bacteria: recognition logic and operating principles.

  2006cited by this paper
• Partitioning between unfolding and release of native domains during ClpXP degradation determines substrate selectivity and partial processing.

  2005cited by this paper
• Localization to the Proteasome Is Sufficient for Degradation*

  2004cited by this paper
• Bivalent tethering of SspB to ClpXP is required for efficient substrate delivery: a protein-design study.

  2004cited by this paper
• Communication between ClpX and ClpP during substrate processing and degradation

  2004cited by this paper
• Nucleotide-dependent substrate handoff from the SspB adaptor to the AAA+ ClpXP protease.

  2004cited by this paper
• Effects of local protein stability and the geometric position of the substrate degradation tag on the efficiency of ClpXP denaturation and degradation.

  2004cited by this paper
• Sculpting the proteome with AAA(+) proteases and disassembly machines.

  2004cited by this paper
• A small-molecule switch for Golgi sulfotransferases.

  2004cited by this paper
• Proteomic discovery of cellular substrates of the ClpXP protease reveals five classes of ClpX-recognition signals.

  2003cited by this paper
• Linkage between ATP consumption and mechanical unfolding during the protein processing reactions of an AAA+ degradation machine.

  2003cited by this paper
• The LOV domain family: photoresponsive signaling modules coupled to diverse output domains.

  2003cited by this paper
• Flexible linkers leash the substrate binding domain of SspB to a peptide module that stabilizes delivery complexes with the AAA+ ClpXP protease.

  2003cited by this paper
• Conditional protein splicing: a new tool to control protein structure and function in vitro and in vivo.

  2003cited by this paper
• A light-switchable gene promoter system

  2002cited by this paper
• Unique and overlapping roles for ZipA and FtsA in septal ring assembly in Escherichia coli

  2002cited by this paper
• Remodeling of yeast genome expression in response to environmental changes.

  2001cited by this paper
• A new method for generating point mutations in bacterial artificial chromosomes by homologous recombination in Escherichia coli.

  2001cited by this paper
• Protacs: Chimeric molecules that target proteins to the Skp1–Cullin–F box complex for ubiquitination and degradation

  2001cited by this paper
• Identification of Critical Staphylococcal Genes Using Conditional Phenotypes Generated by Antisense RNA

  2001cited by this paper
• Global analysis of the genetic network controlling a bacterial cell cycle.

  2000cited by this paper
• A specificity-enhancing factor for the ClpXP degradation machine.

  2000cited by this paper
• A synthetic oscillatory network of transcriptional regulators

  2000cited by this paper
• Crystal and solution structures of an HslUV protease-chaperone complex.

  2000cited by this paper
• Regulation of Escherichia coli cell division genes ftsA and ftsZ by the two‐component system rcsC–rcsB

  1999cited by this paper
• Recruitment of ZipA to the Septal Ring ofEscherichia coli Is Dependent on FtsZ and Independent of FtsA

  1999cited by this paper
• Recruitment of ZipA to the division site by interaction with FtsZ

  1999cited by this paper
• Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans

  1998cited by this paper
• Regulation of transcription of cell division genes in the Escherichia coli dcw cluster

  1998cited by this paper
• A Tn10 derivative (T-POP) for isolation of insertions with conditional (tetracycline-dependent) phenotypes

  1997cited by this paper
• Control of cell division in Escherichia coli: regulation of transcription of ftsQA involves both rpoS and SdiA-mediated autoinduction.

  1996cited by this paper
• Identification of an 11-kDa FKBP12-rapamycin-binding domain within the 289-kDa FKBP12-rapamycin-associated protein and characterization of a critical serine residue.

  1995cited by this paper
• Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter

  1995cited by this paper
• pKSS--a second-generation general purpose cloning vector for efficient positive selection of recombinant clones.

  1994cited by this paper
• Transcription of ftsZ oscillates during the cell cycle of Escherichia coli.

  1993cited by this paper
• A factor that positively regulates cell division by activating transcription of the major cluster of essential cell division genes of Escherichia coli.

  1991cited by this paper
• Regulation of bacterial cell division: temperature-sensitive mutants of Escherichia coli that are defective in septum formation

  1975cited by this paper
• lac repressor--operator interaction. II. Effect of galactosides and other ligands.

  1970cited by this paper

• Mismatch type impacts interference and priming activities in the type I-E CRISPR-Cas system

  2026cites this paper
• Targeted protein degradation in Escherichia coli using CLIPPERs

  2025cites this paper
• An open axial channel of the AAA ClpXP protease enhances degradation of specific classes of protein substrates

  2025cites this paper
• Protein overabundance is driven by growth robustness

  2024cites this paper
• Cell factories for biosynthesis of D-glucaric acid: a fusion of static and dynamic strategies

  2024cites this paper
• Degron-Controlled Protein Degradation in Escherichia coli: New Approaches and Parameters.

  2024cites this paper
• Integrating bacterial molecular genetics with chemical biology for renewed antibacterial drug discovery

  2024cites this paper
• Protein overabundance is driven by growth robustness

  2024cites this paper
• Light-dependent modulation of protein localization and function in living bacteria cells

  2024cites this paper
• State-of-the-art in engineering small molecule biosensors and their applications in metabolic engineering.

  2023cites this paper
• Degron-controlled protein degradation in Escherichia coli: New Approaches and Parameters

  2023cites this paper
• Dissecting the Permeability of the Escherichia coli Cell Envelope to a Small Molecule Using Tailored Intensiometric Fluorescent Protein Sensors

  2023cites this paper
• Light-dependent modulation of protein localization and function in living bacteria cells

  2022cites this paper
• The SspB adaptor drives structural changes in the AAA+ ClpXP protease during ssrA-tagged substrate delivery

  2022cites this paper
• Engineering an SspB-mediated degron for novel controllable protein degradation.

  2022cites this paper
• Bacterial degrons in synthetic circuits

  2022cites this paper
• Gradients in gene essentiality reshape antibacterial research.

  2022cites this paper
• A Uniform Benchmark for Testing SsrA-Derived Degrons in the Escherichia coli ClpXP Degradation Pathway

  2021cites this paper
• Gene-specific mutagenesis enables rapid continuous evolution of enzymes in vivo

  2021cites this paper
• Applications of Bacterial Degrons and Degraders — Toward Targeted Protein Degradation in Bacteria

  2021cites this paper
• Enhanced regulation of prokaryotic gene expression by a eukaryotic transcriptional activator

  2021cites this paper
• A uniform benchmark for testing ssrA-derived degrons in the Escherichia coli ClpXP pathway

  2021cites this paper
• ClpXP Degradation System in Escherichia coli, a Study of Its Energy Sources and Its Applications in Managing the Expression Levels of Targeted Membrane and Soluble Proteins

  2021cites this paper
• Elucidation of gene regulatory mechanisms in ageing bacterial colonies and tool development for cell factory optimization

  2020cites this paper
• Recent Advances in Microbial Cell Growth Regulation Strategies for Metabolic Engineering

  2020cites this paper
• Cell-based and cell-free biocatalysis for the production of d-glucaric acid

  2020cites this paper
• An overview of the bacterial SsrA system modulating intracellular protein levels and activities

  2020influential citation
• Molecular tool development for conditional protein degradation in the model bacterium Escherichia coli

  2018cites this paper
• A Small Periplasmic Protein with a Hydrophobic C-Terminal Residue Enhances DegP Proteolysis as a Suicide Activator

  2017cites this paper
• Small-Molecule Modulation of Protein Homeostasis.

  2017cites this paper
• Engineered Photoactivatable Genetic Switches Based on the Bacterium Phage T7 RNA Polymerase.

  2017cites this paper
• Identification of New Degrons in Streptococcus mutans Reveals a Novel Strategy for Engineering Targeted, Controllable Proteolysis

  2017influential citation
• Tripodal Lipoprotein Variants with C-Terminal Hydrophobic Residues Allosterically Modulate Activity of the DegP Protease.

  2017cites this paper
• Foundations and Emerging Paradigms for Computing in Living Cells.

  2016cites this paper
• Peptide Fingerprinting Using Single-Molecule Fluorescence

  2016cites this paper
• N-Terminal-Based Targeted, Inducible Protein Degradation in Escherichia coli

  2016cites this paper
• Conformational Control of Cascade Interference and Priming Activities in CRISPR Immunity.

  2016cites this paper
• Tools and Principles for Microbial Gene Circuit Engineering.

  2016influential citation
• Precision metabolic engineering: The design of responsive, selective, and controllable metabolic systems.

  2015influential citation
• Light-induced protein degradation : a chemical biology approach

  2015cites this paper
• Dynamic control of Escherichia coli central metabolism for improvement of glucaric acid production

  2015cites this paper
• Dynamic knockdown of E. coli central metabolism for redirecting fluxes of primary metabolites.

  2014cites this paper
• Tunable protein degradation in bacteria

  2014cites this paper
• Distinct regulatory mechanisms balance DegP proteolysis to maintain cellular fitness during heat stress

  2014cites this paper
• Control of Protein Function through Regulated Protein Degradation: Biotechnological and Biomedical Applications

  2013cites this paper
• Cage assembly of DegP protease is not required for substrate-dependent regulation of proteolytic activity or high-temperature cell survival

  2012cites this paper