Ultrasound Therapy of Injury Site Modulates Gene and Protein Expressions in the Dorsal Root Ganglion in a Sciatic Nerve Crush Injury Rat Model.

The aim of this study was to verify the effects of ultrasound on dorsal root ganglion (DRG) neurons at the injury site in a rat model of sciatic nerve crush injury. We evaluated the mRNA expression of neurotrophic and pro-inflammatory factors by quantitative reverse transcription polymerase chain reaction 7 and 14 d post-injury. We also evaluated the protein levels of brain-derived neurotrophic factor (BDNF) 7 and 14 d post-injury. Axon regeneration and motor function analyses were performed 21 days after injury to confirm the facilitative effect of ultrasound on nerve regeneration. In the ultrasound group, BDNF and interleukin-6 mRNA expression of the DRG was significantly reduced 7 d post-injury. Compared with the sham group, the BDNF protein expression of the DRG in the ultrasound group remained at a higher level 14 d post-injury. Motor function, myelinated fiber density and myelin sheath thickness were significantly higher in the ultrasound group than in the sham group 21 d post-injury. These results indicate that ultrasound therapy at the injury site promotes nerve regeneration and modulates gene and protein expression in the DRG of a rat model of a sciatic nerve crush injury.

• Publication year

  2022

• Venue

  Ultrasound in Medicine and Biology

• Publication date

  2022-09-01

• Fields of study

  Medicine, Engineering

• Identifiers
  DOI 10.1016/j.ultrasmedbio.2022.07.015PMID 36180311
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Investigating the Optimal Initiation Time of Ultrasound Therapy for Peripheral Nerve Regeneration after Axonotmesis in Rats.

  2021cited by this paper
• Low-Intensity Pulsed Ultrasound Prompts Both Functional and Histologic Improvements While Upregulating the Brain-Derived Neurotrophic Factor Expression after Sciatic Crush Injury in Rats.

  2021cited by this paper
• Peripheral Nerve Regeneration and Muscle Reinnervation

  2020cited by this paper
• Peripheral nerve injury and myelination: Potential therapeutic strategies

  2020cited by this paper
• Biological characteristics of dynamic expression of nerve regeneration related growth factors in dorsal root ganglia after peripheral nerve injury

  2020cited by this paper
• Ultrasound therapy with optimal intensity facilitates peripheral nerve regeneration in rats through suppression of pro-inflammatory and nerve growth inhibitor gene expression

  2020cited by this paper
• Neurotrophic Factors: An Overview.

  2018cited by this paper
• Reduced inflammatory factor expression facilitates recovery after sciatic nerve injury in TLR4 mutant mice

  2018cited by this paper
• Functional evaluation outcomes correlate with histomorphometric changes in the rat sciatic nerve crush injury model: A comparison between sciatic functional index and kinematic analysis

  2018cited by this paper
• The Effect of Low-Intensity Ultrasound on Brain-Derived Neurotropic Factor Expression in a Rat Sciatic Nerve Crushed Injury Model.

  2017cited by this paper
• HuD-mediated distinct BDNF regulatory pathways promote regeneration after nerve injury.

  2017cited by this paper
• The neuroimmunology of degeneration and regeneration in the peripheral nervous system.

  2015cited by this paper
• Nerve physiology: mechanisms of injury and recovery.

  2013cited by this paper
• Advances in peripheral nerve regeneration

  2013cited by this paper
• Extrinsic cellular and molecular mediators of peripheral axonal regeneration

  2012cited by this paper
• Wallerian degeneration: gaining perspective on inflammatory events after peripheral nerve injury

  2011cited by this paper
• Comparisons of outcomes from repair of median nerve and ulnar nerve defect with nerve graft and tubulization: a meta-analysis.

  2011cited by this paper
• Repairing injured peripheral nerves: Bridging the gap.

  2010cited by this paper
• Upgraded nerve growth factor expression induced by low-intensity continuous-wave ultrasound accelerates regeneration of neurotometicly injured sciatic nerve in rats.

  2010cited by this paper
• The Incidence of Peripheral Nerve Injury in Extremity Trauma

  2008cited by this paper
• Interactions between Schwann cells and macrophages in injury and inherited demyelinating disease

  2008cited by this paper
• Peripherally-Derived BDNF Promotes Regeneration of Ascending Sensory Neurons after Spinal Cord Injury

  2008cited by this paper
• Volumetric magnetic resonance imaging of dorsal root ganglia for the objective quantitative assessment of neuron death after peripheral nerve injury.

  2007cited by this paper
• Therapeutic applications of ultrasound.

  2007cited by this paper
• Therapeutic applications of ultrasound.

  2007cited by this paper
• The Cytokine Interleukin-6 Is Sufficient But Not Necessary to Mimic the Peripheral Conditioning Lesion Effect on Axonal Growth

  2006cited by this paper
• Conditioning Injury-Induced Spinal Axon Regeneration Fails in Interleukin-6 Knock-Out Mice

  2004cited by this paper
• Increased expression and localization of the RNA-binding protein HuD and GAP-43 mRNA to cytoplasmic granules in DRG neurons during nerve regeneration.

  2003cited by this paper
• Inaugural Article: Neurotrophins are key mediators of the myelination program in the peripheral nervous system

  2001cited by this paper
• Prostaglandin Levels in Stimulated Macrophages Are Controlled by Phospholipase A2-activating Protein and by Activation of Phospholipase C and D*

  2001cited by this paper
• Traumatic injury to peripheral nerves.

  2000cited by this paper
• Neuronal injury increases retrograde axonal transport of the neurotrophins to spinal sensory neurons and motor neurons via multiple receptor mechanisms.

  1998cited by this paper
• The NeurotroDhins BDNF, NT‐3 and NT‐4/5 Promote Survival and Morphological and Biochemical Differentiation of Striatal Neurons In Vitro

  1995cited by this paper
• Differential expression of mRNAs for neurotrophins and their receptors after axotomy of the sciatic nerve

  1993cited by this paper
• The neurotrophins BDNF, NT-3, and NGF display distinct patterns of retrograde axonal transport in peripheral and central neurons.

  1992cited by this paper
• Time-dependent differences in the increase in GAP-43 expression in dorsal root ganglion cells after peripheral axotomy.

  1991cited by this paper
• Correlation between GAP43 and nerve growth factor receptors in rat sensory neurons

  1990cited by this paper
• The growth-associated protein GAP-43 appears in dorsal root ganglion cells and in the dorsal horn of the rat spinal cord following peripheral nerve injury.

  1990cited by this paper
• THREE TYPES OF NERVE INJURY

  1943cited by this paper

• Age‑related changes in endoplasmic reticulum stress response‑associated protein expression in rat tibial nerves

  2025cites this paper
• Mitochondria-targeted Sonodynamic Modulation of Neuroinflammation to Protect against Myocardial Ischemia‒Reperfusion Injury.

  2024cites this paper
• Repetitive magnetic stimulation prevents dorsal root ganglion neuron death and enhances nerve regeneration in a sciatic nerve injury rat model

  2024influential citation
• An injectable thermosensitive pluronic F127 loaded-nanohydroxyapatite / Polydopamine for promoting sciatic nerve repair after crush injury.

  2024cites this paper
• Expanding the Targets of Therapeutic Electrophysical Stimulation - For the Advancement of Peripheral Nerve Regenerative Rehabilitation

  2023cites this paper