Association of ScV-LA Virus with Host Protein Metabolism Determined by Proteomics Analysis and Cognate RNA Sequencing

Saccharomyces yeasts are highly dispersed in the environment and microbiota of higher organisms. The yeast killing phenotype, encoded by the viral system, was discovered to be a significant property for host survival. Minor alterations in transcription patterns underpin the reciprocal relationship between LA and M viruses and their hosts, suggesting the fine-tuning of the transcriptional landscape. To uncover the principal targets of both viruses, we performed proteomics analysis of virus-enriched subsets of host proteins in virus type-specific manner. The essential pathways of protein metabolism–from biosynthesis and folding to degradation–were found substantially enriched in virus-linked subsets. The fractionation of viruses allowed separation of virus-linked host RNAs, investigated by high-content RNA sequencing. Ribosomal RNA was found to be inherently associated with LA-lus virus, along with other RNAs essential for ribosome biogenesis. This study provides a unique portrayal of yeast virions through the characterization of the associated proteome and cognate RNAs, and offers a background for understanding ScV-LA viral infection persistency.

• Publication year

  2022

• Venue

  Viruses

• Publication date

  2022-10-25

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.3390/v14112345PMID 36366443PMCID 9697790
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Adaptive Response of Saccharomyces Hosts to Totiviridae L-A dsRNA Viruses Is Achieved through Intrinsically Balanced Action of Targeted Transcription Factors

  2022cited by this paper
• Quantitative Temporal Viromics.

  2021cited by this paper
• Interactions of HIV-1 Capsid with Host Factors and Their Implications for Developing Novel Therapeutics

  2021cited by this paper
• Specificity Determination in Saccharomyces cerevisiae Killer Virus Systems

  2021cited by this paper
• A TMT-Based Quantitative Proteome Analysis to Elucidate the TSWV Induced Signaling Cascade in Susceptible and Resistant Cultivars of Solanum lycopersicum

  2020cited by this paper
• Saccharomyces paradoxus Transcriptional Alterations in Cells of Distinct Phenotype and Viral dsRNA Content

  2020cited by this paper
• Anti-infective nitazoxanide disrupts transcription of ribosome biogenesis-related genes in yeast

  2020cited by this paper
• Protein Interactions Network of Porcine Circovirus Type 2 Capsid With Host Proteins

  2020cited by this paper
• A Review on Viral Metagenomics in Extreme Environments

  2019cited by this paper
• TMT Labeling for the Masses: A Robust and Cost-efficient, In-solution Labeling Approach*

  2019cited by this paper
• Chasing Intracellular Zika Virus Using Proteomics

  2019cited by this paper
• Yeast killer toxins: from ecological significance to application.

  2019cited by this paper
• Identifying the Cellular Interactome of Epstein-Barr Virus Lytic Regulator Zta Reveals Cellular Targets Contributing to Viral Replication

  2019cited by this paper
• Meiotic viral attenuation through an ancestral apoptotic pathway

  2019cited by this paper
• The exoribonuclease Xrn1 is a post-transcriptional negative regulator of autophagy

  2018cited by this paper
• Changes in salivary analytes in canine parvovirus: A high-resolution quantitative proteomic study

  2018cited by this paper
• Comprehensive Profiling of Lysine Acetylome in Baculovirus Infected Silkworm (Bombyx mori) Cells

  2018cited by this paper
• Proteomic Profile Associated With Loss of Spontaneous Human Immunodeficiency Virus Type 1 Elite Control

  2018cited by this paper
• The gut mycobiome of the Human Microbiome Project healthy cohort

  2017cited by this paper
• Different Metabolic Pathways Are Involved in Response of Saccharomyces cerevisiae to L-A and M Viruses

  2017cited by this paper
• Protein Interactions during the Flavivirus and Hepacivirus Life Cycle*

  2017cited by this paper
• Virus purification by CsCl density gradient using general centrifugation

  2017cited by this paper
• The Biology of Pichia membranifaciens Killer Toxins

  2017cited by this paper
• XRN1 Is a Species-Specific Virus Restriction Factor in Yeasts

  2016cited by this paper
• Genome-Wide Mapping of Binding Sites Reveals Multiple Biological Functions of the Transcription Factor Cst6p in Saccharomyces cerevisiae

  2016cited by this paper
• Fungal microbiota dysbiosis in IBD

  2016cited by this paper
• Diphosphates at the 5′ end of the positive strand of yeast L‐A double‐stranded RNA virus as a molecular self‐identity tag

  2016cited by this paper
• Proteomic approaches to uncovering virus–host protein interactions during the progression of viral infection

  2016cited by this paper
• A population study of killer viruses reveals different evolutionary histories of two closely related Saccharomyces sensu stricto yeasts

  2015cited by this paper
• Interactions between chromosomal and nonchromosomal elements reveal missing heritability

  2014cited by this paper
• Mass spectrometry-based proteomic approaches for discovery of HIV-host interactions.

  2014cited by this paper
• RNA decay machines: the exosome.

  2013cited by this paper
• L-A-lus, a New Variant of the L-A Totivirus Found in Wine Yeasts with Klus Killer Toxin-Encoding Mlus Double-Stranded RNA: Possible Role of Killer Toxin-Encoding Satellite RNAs in the Evolution of Their Helper Viruses

  2013cited by this paper
• Yeast response to LA virus indicates coadapted global gene expression during mycoviral infection.

  2013influential reference
• Incidence of symbiotic dsRNA 'killer' viruses in wild and domesticated yeast.

  2013cited by this paper
• Activation of 5′-3′ exoribonuclease Xrn1 by cofactor Dcs1 is essential for mitochondrial function in yeast

  2012cited by this paper
• Toward system-level understanding of baculovirus–host cell interactions: from molecular fundamental studies to large-scale proteomics approaches

  2012cited by this paper
• Contribution of MS-Based Proteomics to the Understanding of Herpes Simplex Virus Type 1 Interaction with Host Cells

  2012cited by this paper
• Cap-snatching mechanism in yeast L-A double-stranded RNA virus

  2011cited by this paper
• Yeast Double-stranded RNA Virus L-A Deliberately Synthesizes RNA Transcripts with 5′-Diphosphate*

  2010cited by this paper
• A transcriptional activator is part of an SCF ubiquitin ligase to control degradation of its cofactors.

  2010cited by this paper
• Laboratory procedures to generate viral metagenomes

  2009cited by this paper
• Elevated copy number of L-A virus in yeast mutant strains defective in ribosomal stalk.

  2007cited by this paper
• Yeast viral killer toxins: lethality and self-protection

  2006cited by this paper
• Proteomics Analysis of the Tombusvirus Replicase: Hsp70 Molecular Chaperone Is Associated with the Replicase and Enhances Viral RNA Replication

  2006cited by this paper
• Hypovirulence: Mycoviruses at the fungal–plant interface

  2005cited by this paper
• Yeast prions [URE3] and [PSI+] are diseases.

  2005cited by this paper
• Identification of Functionally Important Amino Acids of Ribosomal Protein L3 by Saturation Mutagenesis

  2005cited by this paper
• A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length.

  2004cited by this paper
• Physiological and technological aspects of large-scale heterologous-protein production with yeasts

  2004cited by this paper
• Origin, Adaptation and Evolutionary Pathways of Fungal Viruses

  2004cited by this paper
• An automated method for finding molecular complexes in large protein interaction networks

  2003cited by this paper
• Decapping and Decay of Messenger RNA Occur in Cytoplasmic Processing Bodies

  2003cited by this paper
• Cytoscape: a software environment for integrated models of biomolecular interaction networks.

  2003cited by this paper
• Delayed rRNA Processing Results in Significant Ribosome Biogenesis and Functional Defects

  2003cited by this paper
• Decreased peptidyltransferase activity correlates with increased programmed -1 ribosomal frameshifting and viral maintenance defects in the yeast Saccharomyces cerevisiae.

  2003cited by this paper
• KEM1 is involved in filamentous growth of Saccharomyces cerevisiae.

  2002cited by this paper
• New Insights into the Pleiotropic Drug Resistance Network from Genome-Wide Characterization of the YRR1 Transcription Factor Regulation System

  2002cited by this paper
• NatC Nα-terminal Acetyltransferase of Yeast Contains Three Subunits, Mak3p, Mak10p, and Mak31p*

  2001cited by this paper
• Active-Site Mutations in the Xrn1p Exoribonuclease of Saccharomyces cerevisiae Reveal a Specific Role in Meiosis

  1999cited by this paper
• Yeast virus propagation depends critically on free 60S ribosomal subunit concentration

  1995influential reference
• Preparation of Protein Extracts from Yeast

  1993cited by this paper
• Evidence that the SKI antiviral system of Saccharomyces cerevisiae acts by blocking expression of viral mRNA

  1993cited by this paper
• Double-stranded and single-stranded RNA viruses of Saccharomyces cerevisiae.

  1992influential reference
• MAK10, a glucose-repressible gene necessary for replication of a dsRNA virus of Saccharomyces cerevisiae, has T cell receptor alpha-subunit motifs.

  1992cited by this paper
• Overproduction of yeast viruslike particles by strains deficient in a mitochondrial nuclease

  1989cited by this paper
• Accumulation of viruslike particles in a yeast mutant lacking a mitochondrial pore protein.

  1989cited by this paper
• Internal and terminal cis‐acting sites are necessary for in vitro replication of the L‐A double‐stranded RNA virus of yeast.

  1989cited by this paper
• Replicase of L-A virus-like particles of Saccharomyces cerevisiae. In vitro conversion of exogenous L-A and M1 single-stranded RNAs to double-stranded form.

  1988cited by this paper
• Nucleotide phosphotransferase, nucleotide kinase and inorganic pyrophosphatase activities of killer virions of yeast

  1987cited by this paper
• L-A double-stranded RNA viruslike particle replication cycle in Saccharomyces cerevisiae: particle maturation in vitro and effects of mak10 and pet18 mutations

  1987cited by this paper
• Genetic Control of L-a and L-(Bc) Dsrna Copy Number in Killer Systems of SACCHAROMYCES CEREVISIAE.

  1984cited by this paper
• Isolation of seventeen proteins and amino-terminal amino acid sequences of eight proteins from cytoplasmic ribosomes of yeast.

  1982cited by this paper
• Co-curing of plasmids affecting killer double-stranded RNAs of Saccharomyces cerevisiae: [HOK], [NEX], and the abundance of L are related and further evidence that M1 requires L

  1982cited by this paper

• Essential oils expose diverse targets on non-enveloped ScV-L-A totivirus

  2025influential citation
• A removable and cosmopolitan dsRNA Toti-like virus causes asymptomatic but productive infection in a model diatom strain

  2024cites this paper
• Investigation of plant virus-like particle formation in bacterial and yeast expression systems

  2024cites this paper
• The Killer Saccharomyces cerevisiae Toxin: From Origin to Biomedical Research

  2024cites this paper
• Recent Advances in the Yeast Killer Systems Research

  2023cites this paper