Formononetin Inhibits Hepatic I/R-Induced Injury through Regulating PHB2/PINK1/Parkin Pathway

Formononetin (FN), an isoflavone compound mainly isolated from soy and red clover, had showed its anti‐inflammation, antioxidative effects in some degenerative diseases and cholestasis. However, the role of FN in protecting ischemia/reperfusion‐ (I/R‐) induced liver injury and the possible mechanism were unclear. In this study, effects of FN on liver injury were investigated in a rat hepatic I/R model; further, mitophagy‐related proteins were measured by immunoblotting or immunofluorescence. The possible roles of PHB2 and PINK1 in regulating mitophagy by FN were verified using adeno‐associated virus knockdown. The results showed that FN had protective effects against hepatic I/R injury through regulating PINK1/Parkin‐regulated mitophagy. Further, we found that FN inhibited PARL expression and prevented PGAM5 cropped by increasing the expression of PHB2. The knockdown of PINK1 or PHB2 both abolished the protective effects of FN. Taken together, our findings indicated that the isoflavone compound FN promoted PHB2/PINK1/Parkin‐mediated mitophagy pathway to protect liver from I/R‐induced injury. These results provided novel insights into the potential prevention strategies of FN and its underlying mechanisms.

• Publication year

  2022

• Venue

  Oxidative Medicine and Cellular Longevity

• Publication date

  2022-12-02

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1155/2022/6481192PMID 36506934PMCID 9734001
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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