β-Galactoside-Binding Protein (βGBP) Alters the Cell Cycle, Up-Regulates Expression of the α- and β-Chains of the IFN-γ Receptor, and Triggers IFN-γ-Mediated Apoptosis of Activated Human T Lymphocytes

In this paper, the effects of β-galactoside binding protein (βGBP), the LGALS1 gene product, on the cell cycle progression and expansion of activated human T lymphocytes were studied. βGBP drastically inhibits the IL-2 induced proliferation of PHA-activated T lymphocytes as well as the IL-2 independent proliferation of malignant T lymphocytes by arresting them in the S and G2/M phases of the cell cycle. In addition, βGBP up-regulates the expression of both the α- and the β-chains of the IFN-γR on activated T lymphocyte membrane. None of these effects depend on sugar binding: saturating amounts of lactose do not affect the cell cycle block nor IFN-γR up-modulation. The increased expression of both chains renders βGBP-treated T lymphoblasts sensitive to IFN-γ-induced apoptosis. Taken as a whole, these findings suggest that βGBP plays an important immunoregulatory role by switching off T lymphocyte effector functions. They also provide the first evidence of up-modulation of IFN-γR expression on T lymphocytes by a negative cell growth regulator.

• Publication year

  1998

• Venue

  Journal of Immunology

• Publication date

  1998-09-01

• Fields of study

  Not labeled

• Identifiers
  DOI 10.4049/jimmunol.161.5.2114
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Induction of human T helper cell type 1 differentiation results in loss of IFN-gamma receptor beta-chain expression.

  1997cited by this paper
• Galectin-1, an Endogenous Lectin Produced by Thymic Epithelial Cells, Induces Apoptosis of Human Thymocytes

  1997cited by this paper
• Switching on of the proliferation or apoptosis of activated human T lymphocytes by IFN-gamma is correlated with the differential expression of the alpha- and beta-chains of its receptor.

  1996cited by this paper
• Human thymic epithelial cells express an endogenous lectin, galectin-1, which binds to core 2 O-glycans on thymocytes and T lymphoblastoid cells

  1995cited by this paper
• Apoptosis of T cells mediated by galectin-1

  1995cited by this paper
• Ligand-Induced Autoregulation of IFN-γ Receptor β Chain Expression in T Helper Cell Subsets

  1995cited by this paper
• Lack of interferon gamma receptor beta chain and the prevention of interferon gamma signaling in TH1 cells.

  1995cited by this paper
• Single-step procedure for labeling DNA strand breaks with fluorescein- or BODIPY-conjugated deoxynucleotides: detection of apoptosis and bromodeoxyuridine incorporation.

  1995cited by this paper
• Cell cycle control of apoptosis in human leukemic T cells.

  1995cited by this paper
• Galectin-1, a β-Galactoside-binding Lectin in Chinese Hamster Ovary Cells

  1995cited by this paper
• Role of cytokines in determining T-lymphocyte function.

  1994cited by this paper
• Galectins: a family of animal beta-galactoside-binding lectins.

  1994cited by this paper
• Environmental signals influencing expression of the IFN-gamma receptor on human T cells control whether IFN-gamma promotes proliferation or apoptosis.

  1994cited by this paper
• Propriocidal apoptosis of mature T lymphocytes occurs at S phase of the cell cycle

  1993cited by this paper
• Mapping on human and mouse chromosomes of the gene for the beta-galactoside-binding protein, an autocrine-negative growth factor.

  1993cited by this paper
• Modulation of interferon‐γ receptor during human T lymphocyte alloactivation

  1993cited by this paper
• lnterleukin-2 programs mouse αβ T lymphocytes for apoptosis

  1991cited by this paper
• A gentle fixation and permeabilization method for combined cell surface and intracellular staining with improved precision in DNA quantification.

  1991cited by this paper
• Interferon gamma plays a critical role in induced cell death of effector T cell: a possible third mechanism of self-tolerance

  1990cited by this paper
• Anti-proliferative effect of IFN-gamma in immune regulation. I. IFN-gamma inhibits the proliferation of Th2 but not Th1 murine helper T lymphocyte clones.

  1988cited by this paper
• Prevention and therapy with electrolectin of experimental autoimmune myasthenia gravis in rabbits

  1983cited by this paper

• Cysteine Oxidation in Human Galectin-1 Occurs Sequentially via a Folded Intermediate to a Fully Oxidized Unfolded Form

  2024cites this paper
• Hepatic transcriptome analyses of juvenile white bass (Morone chrysops) when fed diets where fish meal is partially or totally replaced by alternative protein sources

  2024cites this paper
• Galectin-1 promotes angiogenesis and chondrogenesis during antler regeneration

  2023cites this paper
• Modulatory Role of Galectin-1 in Ulcerative Colitis with Comorbid Metabolic Syndrome

  2023cites this paper
• Regulation of T lymphocyte function by galectin-1 and its dependence on the carbohydrate binding activity of lectin

  2018cites this paper
• Approche multivalente des interactions saccharides - lectines : synthèse de glycoclusters et analyse de la reconnaissance biomoléculaire

  2010cites this paper
• Regulatory Roles of Galectins in the Immune Response

  2005cites this paper
• Galectin-1: A bifunctional regulator of cellular proliferation

  2004cites this paper
• Unlocking the secrets of galectins: a challenge at the frontier of glyco‐immunology

  2002cites this paper
• Galectins: novel anti-inflammatory drug targets

  2002cites this paper
• Interferon‐γ receptor 2 expression as the deciding factor in human T, B, and myeloid cell proliferation or death

  2001cites this paper
• Regulated expression of galectin‐1 during B‐cell activation and implications for T‐cell apoptosis

  2001cites this paper
• “Galectin-1 Induces Central and Peripheral Cell Death: Implications in T-Cell Physiopathology”

  2000cites this paper
• of Apoptosis -Independent Induction g -Dependent and IFN-g Lymphocyte Proliferation Through IFN-Nitric Oxide Suppresses Human T

  1999cites this paper
• Nitric Oxide Suppresses Human T Lymphocyte Proliferation Through IFN-γ-Dependent and IFN-γ-Independent Induction of Apoptosis

  1999cites this paper