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Filamentous structures in the cell envelope are associated with bacteroidetes gliding machinery

Satoshi Shibata,Yuhei O. Tahara,E. Katayama,A. Kawamoto,Takayuki Kato,Yongtao Zhu,Daisuke Nakane,K. Namba,M. Miyata,M. McBride,K. Nakayama

Published 2023 in Communications Biology

ABSTRACT

Electron microscopic visualization and movement analysis of the gliding machinery in Bacteroidetes provide insights into the mechanism of gliding motility, or the ability of these microbes to move on solid surfaces. Many bacteria belonging to the phylum Bacteroidetes move on solid surfaces, called gliding motility. In our previous study with the Bacteroidetes gliding bacterium Flavobacterium johnsoniae , we proposed a helical loop track model, where adhesive SprB filaments are propelled along a helical loop on the cell surface. In this study, we observed the gliding cell rotating counterclockwise about its axis when viewed from the rear to the advancing direction of the cell and revealed that one labeled SprB focus sometimes overtook and passed another SprB focus that was moving in the same direction. Several electron microscopic analyses revealed the presence of a possible multi-rail structure underneath the outer membrane, which was associated with SprB filaments and contained GldJ protein. These results provide insights into the mechanism of Bacteroidetes gliding motility, in which the SprB filaments are propelled along tracks that may form a multi-rail system underneath the outer membrane. The insights may give clues as to how the SprB filaments get their driving force.

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