Molecular characterization and antimicrobial susceptibility profiles of Thai Mycoplasma synoviae isolates

Mycoplasma synoviae (MS) infection is mainly controlled by pathogen-free flocks’ maintenance, medication in infected flocks, and vaccination in high-risk flocks. The effective control strategy requires convenient approach for detecting and differentiating MS strains and reliable drug susceptible evidence for deciding on reasonable antimicrobial usage. This study aimed to characterize the partial vlhA gene of nine Thai MS isolates circulated in chickens in 2020, to verify the PCR-RFLP assay for strain differentiation, and to determine the eight antimicrobial susceptibility profiles using microbroth dilution method. Based on sequence analysis of the partial vlhA gene, Thai MS isolates in 2020 were classified as types E and L with 19 and 35 amino acid lengths, respectively. The developed PCR-RFLP assay could detect and differentiate vaccine and Thai field strains. Most Thai MS isolates in this study were susceptible to tylosin, tylvalosin, tiamulin, doxycycline, oxytetracycline, tilmicosin, and lincomycin-spectinomycin at MIC_50 values of 0.0391, 0.0098, 0.0781, 0.1563, 0.1563, 0.625 and 0.625 μg/mL, respectively; and resistance to enrofloxacin at MIC_50 value of 10 μg/mL. In conclusion, this study revealed diagnostic assays for differentiating MS strains and the antimicrobial susceptibility profiles of Thai MS , which are necessary to design suitable MS control procedures for poultry flocks.

• Publication year

  2023

• Venue

  Scientific Reports

• Publication date

  2023-02-03

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1038/s41598-023-29266-9PMID 36737453PMCID 9898534
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Prevalence of Mycoplasma synoviae and Its Impact on Productivity in Commercial Poultry Farms in Quebec, Canada

  2021cited by this paper
• CLSI Methods Development and Standardization Working Group Best Practices for Evaluation of Antimicrobial Susceptibility Tests

  2018cited by this paper
• Antibiotic susceptibility profiles of Mycoplasma synoviae strains originating from Central and Eastern Europe

  2017cited by this paper
• Characterization of Thai Mycoplasma synoviae Isolates by Sequence Analysis of Partial vlhA Gene

  2016cited by this paper
• Eradication of Mycoplasma synoviae from a multi-age broiler breeder farm using antibiotics therapy.

  2015cited by this paper
• Molecular typing of Iranian field isolates Mycoplasma synoviae and their differentiation from the live commercial vaccine strain MS-H using vlhA gene

  2014cited by this paper
• Is Mycoplasma synoviae outrunning Mycoplasma gallisepticum? A viewpoint from the Netherlands

  2014cited by this paper
• The Genotyping Potential of the Mycoplasma synoviae vlhA Gene

  2012cited by this paper
• Mycoplasma synoviae cell invasion: elucidation of the Mycoplasma pathogenesis in chicken.

  2011cited by this paper
• Editorial: assessing the antimicrobial susceptibility of bacteria obtained from animals.

  2010cited by this paper
• Revised Mycoplasma synoviae vlhA PCRs

  2010cited by this paper
• Treatment of Eggshell Abnormalities and Reduced Egg Production Caused by Mycoplasma synoviae Infection

  2010cited by this paper
• Assessing the antimicrobial susceptibility of bacteria obtained from animals.

  2010cited by this paper
• Vaccines for Mycoplasma diseases in animals and man.

  2009cited by this paper
• Induction of eggshell apex abnormalities by Mycoplasma synoviae: field and experimental studies

  2009cited by this paper
• Development and evaluation of an improved diagnostic PCR for Mycoplasma synoviae using primers located in the haemagglutinin encoding gene vlhA and its value for strain typing.

  2009cited by this paper
• Surveillance of Mycoplasma synoviae Infection in Mixed Thai Native Chickens in the Area of Nakornpathom Province

  2009cited by this paper
• Probiotics

  2009cited by this paper
• Effect of a live Mycoplasma synoviae vaccine on the production of eggshell apex abnormalities induced by a M. synoviae infection preceded by an infection with infectious bronchitis virus D1466

  2009cited by this paper
• In vitro susceptibilities to fluoroquinolones in current and archived Mycoplasma gallisepticum and Mycoplasma synoviae isolates from meat-type turkeys.

  2008cited by this paper
• In vitro antibiotic susceptibility of Dutch Mycoplasma synoviae field isolates originating from joint lesions and the respiratory tract of commercial poultry

  2008cited by this paper
• Susceptibilities of Mycoplasma gallisepticum and Mycoplasma synoviea Isolates to Antimicrobial Agents in vitro

  2008cited by this paper
• Control of Avian Mycoplasma Infections in Commercial Poultry

  2008cited by this paper
• Molecular characterization and determination of antimicrobial resistance of Mycoplasma gallisepticum isolated from chickens.

  2007cited by this paper
• Classification of Mycoplasma synoviae strains using single-strand conformation polymorphism and high-resolution melting-curve analysis of the vlhA gene single-copy region.

  2007cited by this paper
• Persistence of Mycoplasma synoviae in hens after two enrofloxacin treatments and detection of mutations in the parC gene.

  2006cited by this paper
• The effect of a live vaccine on the horizontal transmission of Mycoplasma gallisepticum

  2006cited by this paper
• Systemic Mycoplasma synoviae infection in broiler chickens

  2005cited by this paper
• Specific Detection and Typing of Mycoplasma synoviae Strains in Poultry with PCR and DNA Sequence Analysis Targeting the Hemagglutinin Encoding Gene vlhA

  2004cited by this paper
• Aerosol-induced Mycoplasma synoviae arthritis: the synergistic effect of infectious bronchitis virus infection

  2004cited by this paper
• Apparent eradication of Mycoplasma synoviae in broiler breeders subjected to intensive antibiotic treatment directed to control Escherichia coli

  2003cited by this paper
• An alternative for antibiotic se in poultry: probiotics

  2003cited by this paper
• In vitro development of resistance to enrofloxacin, erythromycin, tylosin, tiamulin and oxytetracycline in Mycoplasma gallisepticum, Mycoplasma iowae and Mycoplasma synoviae.

  2002cited by this paper
• In vitro susceptibility of avian mycoplasmas to enrofloxacin, sarafloxacin, tylosin, and oxytetracycline.

  2001cited by this paper
• Monitoring Mycoplasma gallisepticum and Mycoplasma synoviae infection in breeder chickens after treatment with enrofloxacin.

  2001cited by this paper
• Molecular basis of the length variation in the N-terminal part of Mycoplasma synoviae hemagglutinin.

  2001cited by this paper
• Guidelines and recommendations for antimicrobial minimum inhibitory concentration (MIC) testing against veterinary mycoplasma species. International Research Programme on Comparative Mycoplasmology.

  2000cited by this paper
• Infection and immunity in broiler chicken breeders vaccinated with a temperature-sensitive mutant of Mycoplasma gallisepticum and impact on performance of offspring.

  2000cited by this paper
• Field evaluation of the safety and efficacy of a temperature-sensitive Mycoplasma synoviae live vaccine.

  1998cited by this paper
• Pathogenicity of Mycoplasma synoviae in Broiler Chickens

  1998cited by this paper
• Mycoplasmas in the etiology of multifactorial respiratory disease.

  1998cited by this paper
• Molecular epidemiologic investigations of Mycoplasma gallisepticum conjunctivitis in songbirds by random amplified polymorphic DNA analyses.

  1997cited by this paper
• Mycoplasmoses in poultry.

  1996cited by this paper
• Development and application of a polymerase chain reaction assay for Mycoplasma synoviae.

  1993cited by this paper
• Effect of medicated feed on tracheal infection and population of Mycoplasma gallisepticum in chickens.

  1986cited by this paper
• Microtechnique for Most-Probable-Number Analysis

  1977cited by this paper
• Theory and Practice in Experimental Bacteriology.

  1971cited by this paper

• Tylvalosin pharmacokinetics, tissue residues, and withdrawal intervals following oral and intravenous administration in geese (Anser anser domesticus), domestic pigeons (Columba livia), and Japanese quail (Coturnix japonica).

  2026cites this paper
• A high content (30%) stable doxycycline hyclate drinking-water solution for broilers: preparation, stability, and bioequivalence

  2026cites this paper
• Pharmacokinetic Comparison of Tylvalosin Tartrate Nanocrystal Suspension and Soluble Powder in Broiler Chickens After Oral and Intravenous Administration

  2025cites this paper
• Pharmacokinetics of Tylvalosin Following Intravenous or Oral Administration at Different Doses in Broiler Chickens

  2025influential citation
• Berberine Inhibits Mycoplasma synoviae Infection by Suppressing PIK3CA-Dependent Inflammatory and Apoptotic Responses in Avian Macrophages.

  2025cites this paper
• Different media affect the growth and antimicrobial minimum inhibitory concentrations of Mycoplasma gallisepticum and Mycoplasma synoviae.

  2025cites this paper
• The novel pleuromutilin derivative 22–((4-((4-nitrophenyl)acetamido)phenyl)thio)deoxy pleuromutilin possesses robust anti-mycoplasma activity both in vitro and in vivo

  2024cites this paper
• Molecular assay for detecting MS-H vaccine strain and immune response mechanisms in chickens receiving one or two doses of live MS-H vaccine

  2023influential citation
• The Thai Journal of Veterinary Medicine The Thai

  year unknowninfluential citation