Multiple Bioactivities of Peptides from Hydrolyzed Misgurnus anguillicaudatus

Misgurnus anguillicaudatus (loach) is a widely distributed benthic fish in Asia. In this study, the alkaline protease was used to hydrolyze loach, and the hydrolysate products of different molecular weights were obtained by membrane separation. In vitro antioxidant assays showed that the <3 kDa fraction (SLH-1) exhibited the strongest antioxidant activity (DPPH, hydroxyl radical and superoxide radical scavenging ability, and reducing power), while SLH-1 was purified by gel filtration chromatography, and peptide sequences were identified by LC-MS/MS. A total of six peptides with antioxidant activity were identified, namely SERDPSNIKWGDAGAQ (D-1), TVDGPSGKLWR (D-2), NDHFVKL (D-3), AFRVPTP (D-4), DAGAGIAL (D-5), and VSVVDLTVR (D-6). In vitro angiotensin-converting enzyme (ACE) inhibition assay and pancreatic cholesterol esterase (CE) inhibition assay, peptide D-4 (IC50 95.07 μg/mL, 0.12 mM) and D-2 inhibited ACE, and peptide D-2 (IC50 3.19 mg/mL, 2.62 mM), D-3, and D-6 acted as pancreatic CE inhibitors. The inhibitory mechanisms of these peptides were investigated by molecular docking. The results showed that the peptides acted by binding to the key amino acids of the catalytic domain of enzymes. These results could provide the basis for the nutritional value and promote the type of healthy products from hydrolyzed loach.

• Publication year

  2023

• Venue

  Molecules

• Publication date

  2023-03-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.3390/molecules28062589PMID 36985560PMCID 10053552
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Extraction, identification and anti-photoaging activity evaluation of collagen peptides from silver carp (Hypophthalmichthys molitrix) skin

  2023cited by this paper
• Structural characterization and angiotensin-converting enzyme (ACE) inhibitory mechanism of Stropharia rugosoannulata mushroom peptides prepared by ultrasound

  2022cited by this paper
• Purification, identification, and antioxidative mechanism of three novel selenium-enriched oyster antioxidant peptides.

  2022influential reference
• Isolation and characterization, antioxidant, and antihypertensive activity of novel bioactive peptides derived from hydrolysis of King Boletus mushroom

  2022influential reference
• Novel ACE inhibitory, antioxidant and α-glucosidase inhibitory peptides identified from fermented rubing cheese through peptidomic and molecular docking

  2022cited by this paper
• Development of antioxidant peptides from brewers’ spent grain proteins

  2022cited by this paper
• Characterization of ACE inhibitory and antioxidant peptides in yak and cow milk hard chhurpi cheese of the Sikkim Himalayan region

  2022cited by this paper
• Monocarbonyl Curcumin Analogues as Potent Inhibitors against Human Glutathione Transferase P1-1

  2022cited by this paper
• Purification and fractionation of bioactive peptides through membrane filtration: A critical and application review

  2022cited by this paper
• Novel bioactive peptides from ginkgo biloba seed protein and evaluation of their α-glucosidase inhibition activity.

  2022cited by this paper
• Identification and Immunomodulatory Effect on Immunosuppressed Mice of Selenium-Enriched Peptides of Egg White.

  2022cited by this paper
• Novel angiotensin-converting enzyme and pancreatic lipase oligopeptide inhibitors from fermented rice bran

  2022cited by this paper
• Generation of novel antioxidant peptides from silver carp muscle hydrolysate: Gastrointestinal digestion stability and transepithelial absorption property.

  2022cited by this paper
• Immunomodulatory effects of egg white peptides on immunosuppressed mice and sequence identification of immunomodulatory peptides

  2022cited by this paper
• Identification of peptides in Qingke baijiu and evaluation of its angiotensin converting enzyme (ACE) inhibitory activity and stability.

  2022cited by this paper
• Novel antioxidant peptides from protein hydrolysates of scallop (Argopecten irradians) mantle using enzymatic and microbial methods: Preparation, purification, identification and characterization

  2022cited by this paper
• Identification of angiotensin converting enzyme (ACE) inhibitory and antioxidant peptides derived from Pixian broad bean paste

  2021cited by this paper
• Isolation, purification and characterization of the antibacterial, antihypertensive and antioxidative properties of the bioactive peptides in the purified and proteolyzed major storage protein of pigeon pea (Cajanus cajan) seeds

  2021cited by this paper
• Identification and characterization of cholesterol esterase and lipase inhibitory peptides from amaranth protein hydrolysates

  2021cited by this paper
• Exploration of bioactive peptides from various origin as promising nutraceutical treasures: In vitro, in silico and in vivo studies.

  2021influential reference
• Exploring the potential of novel xanthine oxidase inhibitory peptide (ACECD) derived from Skipjack tuna hydrolysates using affinity-ultrafiltration coupled with HPLC-MALDI-TOF/TOF-MS.

  2021influential reference
• Redox changes in obesity, metabolic syndrome, and diabetes

  2021cited by this paper
• Production, characterization and molecular docking of antioxidant peptides from peptidome of kinema fermented with proteolytic Bacillus spp.

  2021cited by this paper
• Preparation and identification of antioxidant peptides from cottonseed proteins.

  2021influential reference
• Insight into Inhibitory Mechanism of PDE4D by Dietary Polyphenols Using Molecular Dynamics Simulations and Free Energy Calculations

  2021cited by this paper
• New insights into the cholesterol esterase- and lipase-inhibiting potential of bioactive peptides from camel whey hydrolysates: Identification, characterization, and molecular interaction.

  2021cited by this paper
• Production, bioactive properties, and potential applications of fish protein hydrolysates: Developments and challenges

  2021cited by this paper
• In silico studies on pancreatic lipase and cholesterol esterase inhibitor 2, 6-di-tert-butyl phenol: A novel molecule for Antiobesity

  2021cited by this paper
• Characterization and film-forming properties of acid soluble collagens from different by-products of loach (Misgurnus anguillicaudatus)

  2021cited by this paper
• A comparative investigation into novel cholesterol esterase and pancreatic lipase inhibitory peptides from cow and camel casein hydrolysates generated upon enzymatic hydrolysis and in-vitro digestion.

  2021cited by this paper
• Structure-guided discovery of antioxidant peptides bounded to the Keap1 receptor as hunter for potential dietary antioxidants.

  2021influential reference
• Targeting PirAvp and PirBvp Toxins of Vibrio parahaemolyticus with Oilseed Peptides: An In Silico Approach

  2021cited by this paper
• High-degree hydrolysis sea cucumber peptides improve exercise performance and exert antifatigue effect via activating the NRF2 and AMPK signaling pathways in mice

  2021cited by this paper
• In-vitro Angiotensin converting enzyme (ACE), antioxidant activity and some functional properties of silver catfish (Pangasius sp.) protein hydrolysate by ultrafiltration

  2021cited by this paper
• Antioxidant mechanism, antibacterial activity, and functional characterization of peptide fractions obtained from barred mackerel gelatin with a focus on application in carbonated beverages.

  2020cited by this paper
• Studies on the bioactivities and molecular mechanism of antioxidant peptides by 3D-QSAR, in vitro evaluation and molecular dynamic simulations.

  2020cited by this paper
• Purification and characterization of novel antioxidant peptides from duck breast protein hydrolysates

  2020influential reference
• Involvement of Nrf2 and Keap1 in the activation of antioxidant responsive element (ARE) by chemopreventive agent peptides from soft-shelled turtle

  2020cited by this paper
• Optimization of pea protein hydrolysate preparation and purification of antioxidant peptides based on an in silico analytical approach

  2020influential reference
• Milk-derived bioactive peptides exhibit antioxidant activity through the Keap1-Nrf2 signaling pathway

  2020cited by this paper
• Identification and characterization of novel antioxidant peptides from mackerel (Scomber japonicus) muscle protein hydrolysates.

  2020cited by this paper
• The novel peptides ICRD and LCGEC screened from tuna roe show antioxidative activity via Keap1/Nrf2-ARE pathway regulation and gut microbiota modulation.

  2020cited by this paper
• Isolation and identification of cholesterol esterase and pancreatic lipase inhibitory peptides from brewer's spent grain by consecutive chromatography and mass spectrometry.

  2020cited by this paper
• Recent progress in Keap1-Nrf2 protein-protein interaction inhibitors.

  2020cited by this paper
• In Vivo/In Vitro Properties of Novel Antioxidant Peptide from Pinctada fucata

  2020cited by this paper
• Identification of novel antioxidant peptides from snakehead (Channa argus) soup generated during gastrointestinal digestion and insights into the anti-oxidation mechanisms.

  2020influential reference
• A new dual-peptide strategy for enhancing antioxidant activity and exploring the enhancement mechanism.

  2019cited by this paper
• Polypeptides extracted from Eupolyphaga sinensis walker via enzymic digestion alleviate UV radiation-induced skin photoaging.

  2019cited by this paper
• Identification and molecular docking study of novel cholesterol esterase inhibitory peptides from camel milk proteins.

  2019cited by this paper
• Regulatory requirements of bioactive peptides (protein hydrolysates) from food proteins

  2019cited by this paper
• Antioxidant activity of Sind sardine hydrolysates with pistachio green hull (PGH) extracts

  2019cited by this paper
• Functional characterization and biotechnological potential of exopolysaccharide produced by Lactobacillus rhamnosus strains isolated from human breast milk

  2018cited by this paper
• Effect of farming patterns on the nutrient composition and farming environment of loach, Paramisgurnus dabryanus

  2018cited by this paper
• Production of antioxidant and ACE-inhibitory peptides from Kluyveromyces marxianus protein hydrolysates: Purification and molecular docking

  2017influential reference
• Identification, functional gastrointestinal stability and molecular docking studies of lentil peptides with dual antioxidant and angiotensin I converting enzyme inhibitory activities.

  2017cited by this paper
• Food matrix interaction and bioavailability of bioactive peptides: Two faces of the same coin?

  2017cited by this paper
• A novel antioxidant and ACE inhibitory peptide from rice bran protein: Biochemical characterization and molecular docking study

  2017cited by this paper
• Food-derived multifunctional bioactive proteins and peptides: sources and production

  2015cited by this paper
• cDNA cloning and expression characterization of serum transferrin gene from oriental weatherfish Misgurnus anguillicaudatus.

  2014cited by this paper
• Antioxidant and cryoprotective effects of a tetrapeptide isolated from Amur sturgeon skin gelatin

  2014cited by this paper
• Inhibition strength of short peptides derived from an ACE inhibitory peptide.

  2011cited by this paper
• Antioxidant and antiproliferative activities of loach ( Misgurnus anguillicaudatus ) peptides prepared by papain digestion.

  2011cited by this paper
• In vitro antioxidant activity and in vivo anti-fatigue effect of loach (Misgurnus anguillicaudatus) peptides prepared by papain digestion

  2011cited by this paper
• Purification and identification of antioxidative peptides from loach (Misgurnus anguillicaudatus) protein hydrolysate by consecutive chromatography and electrospray ionization-mass spectrometry.

  2010cited by this paper
• ntioxidative peptides from food proteins : A review

  2010cited by this paper
• Lipid-Lowering mechanisms of grape seed extract (Vitis vinifera L) and its antihyperlidemic activity

  2010cited by this paper
• Effect of degree of hydrolysis on the antioxidant activity of loach (Misgurnus anguillicaudatus) protein hydrolysates.

  2009cited by this paper
• Studies of the Toxicological Potential of Tripeptides (L-Valyl-L-prolyl-L-proline and L-lsoleucyl-L-prolyl-L-proline): III. Single- and/or Repeated-Dose Toxicity of Tripeptides-Containing Lactobacillus helveticus-Fermented Milk Powder and Casein Hydrolysate in Rats

  2005cited by this paper
• Food-derived bioactive peptides--opportunities for designing future foods.

  2003cited by this paper
• Signal transduction by reactive oxygen species in non‐phagocytic cells

  1999cited by this paper

• Novel Angiotensin-Converting Enzyme Inhibitory Peptides from Bungarus multicinctus: Simulated Gastrointestinal Digestion, Identification and Antihypertensive Mechanism

  2026cites this paper
• TLR5-mediated pathogenic bacteria defense and functional maintenance of Misgurnus anguillicaudatus ovary

  2026cites this paper
• Antioxidant property and functionality of protein hydrolysate from Chinese softshell turtle (Pelodiscus sinensis)

  2025cites this paper
• Immune mechanism of n-butanol extract of Clerodendrum bungei against loach infected with Aeromonas hydrophila

  2025cites this paper
• Identifying and Evaluating Antihypertensive and Antioxidant Capacities of the Novel Bi-functional Peptides Isolated from Tilapia Skin

  2025cites this paper
• Isolation and Purification of Novel Antioxidant Peptides from Mussel (Mytilus edulis) Prepared by Marine Bacillus velezensis Z-1 Protease

  2025cites this paper
• ASSESSMENT OF GENETIC VARIATION IN THE POND LOACH MISGURNUS ANGUILLICAUDATUS (CANTOR, 1842), BASED ON COI GENE FRAGMENTS

  2024cites this paper
• Novel Angiotensin-Converting Enzyme-Inhibitory Peptides Obtained from Trichiurus lepturus: Preparation, Identification and Potential Antihypertensive Mechanism

  2024cites this paper
• Effect of dietary supplementation with Leuconostoc mesenteroides DH on the antimicrobial capacity and overall health of juvenile loach (Misgurnus anguillicaudatus)

  2023cites this paper
• Identification, characterization and hypolipidemic effect of novel peptides in protein hydrolysate from Protaetia brevitarsis larvae.

  2023cites this paper
• The transcriptome sequencing analysis reveals immune mechanisms of soybean fermented powder on the loach (Misgurnus anguillicaudatus) in response to Lipopolysaccharide (LPS) infection

  2023cites this paper