Chronological age-related metabolome responses in the dinoflagellate Karenia mikimotoi, can predict future bloom demise

A broad survey of the metabolome over the growth curve of K. mikimotoi identifies glucose to glycine ratio as indicative of dinoflagellate cells entering the late stationary phase, predicting bloom demise. Karenia mikimotoi is a common harmful algal bloom (HAB)-forming dinoflagellate and has caused severe financial loss in aquaculture. There are limited metabolomic studies on dinoflagellate biology. Here, we examined alterations in metabolic profiles over the growth curve of K. mikimotoi under nitrogen or phosphorus deficiency and further explored a key criterion for the diagnosis of late stationary phase to identify when the dinoflagellate cells will enter bloom demise. The results demonstrate the differential expression of metabolites for coping with chronological aging or nutrient deprivation. Furthermore, an increase in the glucose to glycine ratio in the late stationary phase was indicative of dinoflagellate cells entering bloom demise; this was also detected in the cultured diatom, Chaetoceros tenuissimus , indicating that this may be the general criterion for phytoplankton species. Our findings provide insights regarding chronological aging and the criterion for the prediction of phytoplankton bloom demise.

• Publication year

  2023

• Venue

  Communications Biology

• Publication date

  2023-03-15

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1038/s42003-023-04646-zPMID 36922623PMCID 10017670
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Transcriptome responses of the dinoflagellate Karenia mikimotoi driven by nitrogen deficiency.

  2021cited by this paper
• MetaboAnalyst 5.0: narrowing the gap between raw spectra and functional insights

  2021cited by this paper
• Nutrient Deficiency and an Algicidal Bacterium Improved the Lipid Profiles of a Novel Promising Oleaginous Dinoflagellate, Prorocentrum donghaiense, for Biodiesel Production

  2021cited by this paper
• Shining Light on Photosynthesis in the Harmful Dinoflagellate Karenia mikimotoi–Responses to Short-Term Changes in Temperature, Nitrogen Form, and Availability

  2021cited by this paper
• The genome of the diatom Chaetoceros tenuissimus carries an ancient integrated fragment of an extant virus

  2021cited by this paper
• Nutrient Alteration Drives the Impacts of Seawater Acidification on the Bloom-Forming Dinoflagellate Karenia mikimotoi

  2021cited by this paper
• Bioavailability of Organic Phosphorus Compounds to the Harmful Dinoflagellate Karenia mikimotoi

  2021cited by this paper
• Progress and promise of omics for predicting the impacts of climate change on harmful algal blooms.

  2020cited by this paper
• Harmful algal blooms and associated fisheries damage in East Asia: Current status and trends in China, Japan, Korea and Russia.

  2020cited by this paper
• Metabolomics-based approach to explore growth phase-dependent markers in cultured diatom Chaetoceros tenuissimus.

  2019cited by this paper
• Omics Analysis for Dinoflagellates Biology Research

  2019cited by this paper
• A review of karenia mikimotoi: Bloom events, physiology, toxicity and toxic mechanism.

  2019cited by this paper
• Effects of ocean acidification and phosphate limitation on physiology and toxicity of the dinoflagellate Karenia mikimotoi.

  2019cited by this paper
• Effect of various nitrogen conditions on population growth, temporary cysts and cellular biochemical compositions of Karenia mikimotoi

  2017cited by this paper
• A hepatic metabolomics-based diagnostic approach to assess lethal toxicity of dithiocarbamate fungicide polycarbamate in three marine fish species.

  2017cited by this paper
• Rapid quantification of viable cells of the planktonic diatom Chaetoceros tenuissimus and associated RNA viruses in culture

  2016cited by this paper
• Influence of acidification and eutrophication on physiological functions of Conticribra weissflogii and Prorocentrum donghaiense.

  2016cited by this paper
• Phosphorus Deficiency Inhibits Cell Division But Not Growth in the Dinoflagellate Amphidinium carterae

  2016cited by this paper
• Effects of temperature on growth, photophysiology, Rubisco gene expression in Prorocentrum donghaiense and Karenia mikimotoi

  2016cited by this paper
• Amino Acid Catabolism in Plants.

  2015cited by this paper
• Whole-cell response to nitrogen deprivation in the diatom Phaeodactylum tricornutum

  2015cited by this paper
• MS-DIAL: Data Independent MS/MS Deconvolution for Comprehensive Metabolome Analysis

  2015cited by this paper
• Harmful algal blooms and climate change: Learning from the past and present to forecast the future.

  2015cited by this paper
• Photosynthesis and respiration in marine phytoplankton: Relationship with cell size, taxonomic affiliation, and growth phase

  2014cited by this paper
• Amino Acid Biosynthesis Pathways in Diatoms

  2013cited by this paper
• Processes in Microbial Ecology

  2012cited by this paper
• Transcriptome remodeling associated with chronological aging in the dinoflagellate, Karenia brevis.

  2012cited by this paper
• Comparative metabolomics of the diatom Skeletonema marinoi in different growth phases

  2012cited by this paper
• Leveraging metabolomics for functional investigations in sequenced marine diatoms.

  2012cited by this paper
• Molecular ecological responses of the dinoflagellate Karenia mikimotoi to phosphate stress

  2011cited by this paper
• Transcriptomic response of the red tide dinoflagellate, Karenia brevis, to nitrogen and phosphorus depletion and addition

  2011cited by this paper
• Harmful algal blooms and eutrophication: “strategies” for nutrient uptake and growth outside the Redfield comfort zone

  2011cited by this paper
• pROC: an open-source package for R and S+ to analyze and compare ROC curves

  2011cited by this paper
• Molecular ecological responses of dinoflagellate, Karenia mikimotoi to environmental nitrate stress.

  2011cited by this paper
• A Model for Carbohydrate Metabolism in the Diatom Phaeodactylum tricornutum Deduced from Comparative Whole Genome Analysis

  2008cited by this paper
• Metabolomics and fluxomics approaches.

  2008cited by this paper
• Nutrition and Growth Kinetics in Nitrogen-or Phosphorus-limited Cultures of the Noxious Red Tide Dinoflagellate Gymnodinium mikimotoi

  1999cited by this paper
• Aquatic Photosynthesis: Second Edition

  1997cited by this paper
• Leaf Phosphate Status, Photosynthesis, and Carbon Partitioning in Sugar Beet (IV. Changes with Time Following Increased Supply of Phosphate to Low-Phosphate Plants)

  1995cited by this paper
• The determination of nitrogen status in microalgae

  1990cited by this paper
• The ratio of glutamine:glutamate in microalgae: a biomarker for N-status suitable for use at natural cell densities

  1989cited by this paper
• 有害赤潮鞭毛藻Gymnodinium nagasakienseの増殖におよぼす水温,塩分および光強度の影響

  1989cited by this paper
• Photosynthesis, Respiration, and Phosphate Absorption by Carex Aquatilis Ecotypes along Latitudinal and Local Environmental Gradients

  1983cited by this paper
• myo-Inositol:Its Biosynthesis and Metabolism

  1983cited by this paper

• Vegetative-cell morphology changes depending on nutritional conditions in Japanese strains of the noxious red-tide microalgae Chattonella

  2025cites this paper
• Protein and fatty acid assimilation from larvae of black soldier fly Hermetia illucens in diets for red seabream Pagrus major

  2025cites this paper
• Revealing the algicidal characteristics of Maribacter dokdonensis: An investigation into bacterial strain P4 isolated from Karenia mikimotoi bloom water

  2024cites this paper
• Viral infection to the raphidophycean alga Heterosigma akashiwo affects both intracellular organic matter composition and dynamics of a coastal prokaryotic community

  2024cites this paper