TrypTag.org: from images to discoveries using genome-wide protein localisation in Trypanosoma brucei.

TrypTag was a 4-year project to tag the N- and C-termini of almost all Trypanosoma brucei proteins with a fluorescent protein and record the subcellular localisation through images and manual annotation. We highlight the new routes to cell biological discovery this transformative resource is enabling for parasitologists and cell biologists.

• Publication year

  2023

• Venue

  Trends in Parasitology

• Publication date

  2023-03-01

• Fields of study

  Biology, Medicine, Computer Science

• Identifiers
  DOI 10.1016/j.pt.2023.02.008PMID 36925446
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Genome-wide subcellular protein map for the flagellate parasite Trypanosoma brucei

  2023cited by this paper
• A multi-adenylate cyclase regulator at the flagellar tip controls African trypanosome transmission

  2022cited by this paper
• Black-necked spitting cobra (Naja nigricollis) phospholipases A2 may cause Trypanosoma brucei death by blocking endocytosis through the flagellar pocket

  2022cited by this paper
• Nucleolar targeting in an early-branching eukaryote suggests a general mechanism for ribosome protein sorting

  2022cited by this paper
• Stage-specific transcription activator ESB1 regulates monoallelic antigen expression in Trypanosoma brucei

  2022cited by this paper
• An assembly of nuclear bodies associates with the active VSG expression site in African trypanosomes

  2022cited by this paper
• An assembly of nuclear bodies associates with the active VSG expression site in African trypanosomes

  2022cited by this paper
• Black-necked spitting cobra (Naja nigricollis) phospholipases A2 may cause Trypanosoma brucei death by blocking endocytosis through the flagellar pocket

  2021cited by this paper
• Systematic evaluation of horizontal gene transfer between eukaryotes and viruses

  2021cited by this paper
• The Trypanosoma brucei subpellicular microtubule array is organized into functionally discrete subdomains defined by microtubule associated proteins

  2020cited by this paper
• Identification of four unconventional kinetoplastid kinetochore proteins KKT22–25 in Trypanosoma brucei

  2019cited by this paper
• Cellular landmarks of Trypanosoma brucei and Leishmania mexicana

  2019cited by this paper
• Supplementary material from "Identification of four unconventional kinetoplastid kinetochore proteins KKT22–25 in Trypanosoma brucei "

  2019cited by this paper
• Direction of flagellum beat propagation is controlled by proximal/distal outer dynein arm asymmetry

  2018cited by this paper
• A subcellular map of the human proteome

  2017cited by this paper
• TrypTag.org: A Trypanosome Genome-wide Protein Localisation Resource

  2017cited by this paper
• Localization of gene products using a chromosomally tagged GFP‐fusion library in the fission yeast Schizosaccharomyces pombe

  2009cited by this paper
• Global analysis of protein localization in budding yeast

  2003cited by this paper

• A Python module for programmatic access to TrypTag genome-wide subcellular protein localisation data in Trypanosoma brucei

  2026cites this paper
• Axonemal dynein contributions to flagellar beat types and waveforms

  2025cites this paper
• Next generation genetic screens in kinetoplastids

  2025cites this paper
• The SET29 and SET7 proteins of Leishmania donovani exercise non-redundant convergent as well as collaborative functions in moderating the parasite’s response to oxidative stress

  2025cites this paper
• A novel approach to tagging tubulin reveals MT assembly dynamics of the axoneme in Trypanosoma brucei

  2025cites this paper
• Callunene, mitophagy, and flagellum removal in trypanosomatids.

  2025cites this paper
• DRBD18 acts as a transcript-specific RNA editing auxiliary factor in Trypanosoma brucei

  2024cites this paper
• The SET7 protein of Leishmania donovani moderates the parasite’s response to a hostile oxidative environment

  2024cites this paper
• Phenotypic screening reveals a highly selective phthalimide-based compound with antileishmanial activity

  2024cites this paper
• High-throughput prioritization of target proteins for development of new antileishmanial compounds

  2024cites this paper
• Evolutionary cell biology comes of age

  2024cites this paper
• Advances in the cellular biology, biochemistry, and molecular biology of acidocalcisomes

  2023cites this paper
• Trypanosomes as a magnifying glass for cell and molecular biology.

  2023cites this paper
• Spatial confinement of Trypanosoma brucei in microfluidic traps provides a new tool to study free swimming parasites

  2023cites this paper
• The bloodstream form of Trypanosoma brucei displays non-canonical gluconeogenesis

  2023cites this paper