Cellular complexity of the peripheral nervous system: Insights from single-cell resolution

Single-cell RNA sequencing allows the division of cell populations, offers precise transcriptional profiling of individual cells, and fundamentally advances the comprehension of cellular diversity. In the peripheral nervous system (PNS), the application of single-cell RNA sequencing identifies multiple types of cells, including neurons, glial cells, ependymal cells, immune cells, and vascular cells. Sub-types of neurons and glial cells have further been recognized in nerve tissues, especially tissues in different physiological and pathological states. In the current article, we compile the heterogeneities of cells that have been reported in the PNS and describe cellular variability during development and regeneration. The discovery of the architecture of peripheral nerves benefits the understanding of the cellular complexity of the PNS and provides a considerable cellular basis for future genetic manipulation.

• Publication year

  2023

• Venue

  Frontiers in Neuroscience

• Publication date

  2023-03-14

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.3389/fnins.2023.1098612PMID 36998728PMCID 10043217
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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• Peripheral Nerve Single-Cell Analysis Identifies Mesenchymal Ligands that Promote Axonal Growth

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• Deep Sequencing of Somatosensory Neurons Reveals Molecular Determinants of Intrinsic Physiological Properties.

  2019cited by this paper
• Stereotyped transcriptomic transformation of somatosensory neurons in response to injury

  2019cited by this paper
• Transcriptional reprogramming of distinct peripheral sensory neuron subtypes after axonal injury

  2019influential reference
• Satellite glial cells promote regenerative growth in sensory neurons

  2019cited by this paper
• Molecular Architecture of the Mouse Nervous System

  2018cited by this paper
• Single Cell Multi-Omics Technology: Methodology and Application

  2018cited by this paper
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  2018cited by this paper
• Epitranscriptomic m6A Regulation of Axon Regeneration in the Adult Mammalian Nervous System.

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• Enteric glia: the most alimentary of all glia

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• Lineage-dependent spatial and functional organization of the mammalian enteric nervous system

  2017influential reference
• Single-cell RNA-sequencing: The future of genome biology is now

  2017cited by this paper
• Comparative transcriptome profiling of the human and mouse dorsal root ganglia: An RNA-seq-based resource for pain and sensory neuroscience research

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• Specification, plasticity and evolutionary origin of peripheral glial cells.

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• Single-cell sequencing reveals dissociation-induced gene expression in tissue subpopulations

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  2017cited by this paper
• Single-cell RNA sequencing: Technical advancements and biological applications.

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• Developmental diversification of cortical inhibitory interneurons

  2017cited by this paper
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  2017cited by this paper
• The Molecular Fingerprint of Dorsal Root and Trigeminal Ganglion Neurons

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• Intrinsic Control of Axon Regeneration.

  2016cited by this paper
• Single-cell RNA-seq reveals distinct injury responses in different types of DRG sensory neurons

  2016cited by this paper
• Laser capture microscopy coupled with Smart-seq2 for precise spatial transcriptomic profiling

  2016cited by this paper
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• Somatosensory neuron types identified by high-coverage single-cell RNA-sequencing and functional heterogeneity

  2015cited by this paper
• Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets.

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• RNA-Seq Analysis of Human Trigeminal and Dorsal Root Ganglia with a Focus on Chemoreceptors

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• Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells.

  2015influential reference
• Combinatorial labeling of single cells for gene expression cytometry

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• HDAC1 and HDAC2 Control the Specification of Neural Crest Cells into Peripheral Glia

  2014influential reference
• Full-length RNA-seq from single cells using Smart-seq2

  2014cited by this paper
• Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing

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• Smart-seq2 for sensitive full-length transcriptome profiling in single cells

  2013influential reference
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  2013cited by this paper
• CEL-Seq: single-cell RNA-Seq by multiplexed linear amplification.

  2012cited by this paper
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  2012cited by this paper
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  2011cited by this paper
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• Intrinsic control of axon regeneration

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• Labeled lines meet and talk: population coding of somatic sensations.

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• Glial cells.

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• Expression of an inwardly rectifying K+ channel, Kir4.1, in satellite cells of rat cochlear ganglia.

  1999cited by this paper
• Transganglionic transport and binding of the isolectin B4 from Griffonia simplicifolia I in rat primary sensory neurons.

  1994cited by this paper

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