INHIBITORY ACTIVITY OF LUPEOL FROM Cryptolepis oblongifolia (MEINS) SCHLTR ON PARTIALLY PURIFIED PHOSPHOLIAPASE A2 VENOM OF Naja nigricollis (ELAPIDAE)

The research is design to provide scientific evidence for the use of Cryptolepis oblongifolia in traditional treatment of inflammation and also to examine whether it have some snake venom phospholipase A2 inhibition potential. A triterpene was isolated from C. oblongifolia using column chromatography and identified via spectroscopic means. The inhibitory activity of the isolate on phospholipase A2 (PLA2) of Naja nigricollis venom was evaluated. The proton nuclear magnetic resonance (1H NMR) of the isolated compound showed the presence of olefinic protons while the carbon nuclear magnetic resonance (13C NMR) showed the presence of exomethylene carbon atoms. By comparison with the reported literature the compound was found to be lupeol, the inhibitory activity of the isolate on PLA2 of Naja nigricollis venom showed that, the isolate inhibits the PLA2 at dose dependent manner with inhibition binding constant (ki) of 0.015 mg/ml. The activity of the compound isolated supports the local usage of C. oblongifolia in traditional treatment of inflammation.

• Publication year

  2023

• Venue

  FUDMA Journal of Sciences

• Publication date

  2023-02-28

• Fields of study

  Not labeled

• Identifiers
  DOI 10.33003/fjs-2023-0701-1242
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar

• No claims are published for this paper.

• No concepts are published for this paper.

• Journal of Ethnobiology and Ethnomedicine Open Access

  year unknowncited by this paper

• No citing papers are available for this paper.