Enhancing gliotoxins production in deep-sea derived fungus Dichotomocyes cejpii by engineering the biosynthetic pathway.

Gliotoxin can be developed as potent biopesticide. In this study, the positive transcriptional factor gliZ and glutathione-S transferase encoding gene gliG were firstly deleted by CRISPR/Cas9 system, which abolished the production of gliotoxin-like compounds in Dichotomomyces cejpii. CRISPR/dCas9 system targeting promoter of gliG was used to activate the biosynthetic genes in gli cluster. The overexpression of gliZ, gliN and gliG can significantly improve the yield of gliotoxin-like compunds. The gliotoxin yields was improved by 16.38±1.36 fold, 18.98±1.28 fold through gliZ overexpression and gliM deletion in D. cejpii FS110. In addtion, gliN was heterologously expressed in E. coli, the purified GliN can catalyze gliotoxin into methyl-gliotoxin. Furthermore, the binding sequences of GliZ in the promoters of gliG was determined by Dnase footprinting. This study firstly illustrated the transcriptional regulatory mechanism of DcGliZ for the gliotoxin biosynthesis in D. cejpii, and improved the yields of gliotoxins significantly in D. cejpii via biosynthetic approaches.

• Publication year

  2023

• Venue

  Bioresource Technology

• Publication date

  2023-03-01

• Fields of study

  Biology, Medicine, Chemistry, Environmental Science

• Identifiers
  DOI 10.1016/j.biortech.2023.128905PMID 36931443
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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