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SnRNA-seq and mRNA hybridization indicate key bud events and LcFT1 and LcTFL1-2 mRNA transportability during floral transition in litchi.

Ming-Chao Yang,Zi-Chen Wu,Riyao Chen,F. Abbas,G. Hu,Xuming Huang,Wei-Song Guan,Yi-Song Xu,Hui-Cong Wang

Published 2023 in Journal of Experimental Botany

ABSTRACT

In flowering plants, floral induction signals intersect at the shoot apex to modulate meristem determinacy and growth form. Herein, we reported a snRNA-seq analysis of litchi apical buds at different developmental stages. A total of 41,641 nuclei expressing 21,402 genes were analyzed, revealing 35 cell clusters corresponding to 12 broad populations. We signature genes associated with floral transition and propose a model that profile the key events associated with litchi floral meristem identity by analyzing 567 identified floral meristem cells at single cell resolution. Interestingly, snRNA-seq data indicated that all putative FT and TFL1 genes were not expressed in bud nuclei, but significant expressions of them were detected in bud samples using RT-PCR. Based on the expression patterns and gene silencing results, we highlight the critical role of LcTFL1-2 in inhibiting flowering and propose that LcFT1/LcTFL1-2 expression ratio may determine the success flower transition. And the transport of LcFT1 and LcTFL1-2 mRNA from the leaf to the shoot apical meristem was proposed based on in-situ and dot blot hybridization results. These findings allowed for a more comprehensive understanding of the molecular events that occur during the litchi floral transition, as well as the identification of new regulators.

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